Mostrando recursos 1 - 20 de 1.055

  1. Foraging niche segregation between juvenile and adult hawksbill turtles (Eretmochelys imbricata) at Príncipe island, West Africa

    Ferreira, Rogério L.; Ceia, Filipe R.; Borges, Teresa C.; Ramos, Jaime A.; Bolten, Alan B.
    Hawksbill sea turtles are the most tropical sea turtle species with one of the last remaining aggregations in West Africa found on Príncipe Island (1° 37′ N; 7° 23′ E). Here we present for the first time, data on the trophic and foraging ecology of both juvenile and adult hawksbill turtles found in the shallow waters of Príncipe, using stable isotope analyses of carbon (δ13C) and nitrogen (δ15N), and assess any isotopic niche segregation between these two life-stages. Hawksbill turtles were sampled from December 2012 to June 2014, complementary to a snorkeling survey conducted around the entire coastline (ca. 100...

  2. In vitro and in silico approaches to appraise Polygonum maritimum L. as a source of innovative products with anti-ageing potential

    Rodrigues, Maria João; Slusarczyk, Sylwester; Pecio, Łukasz; Matkowski, Adam; Salmas, Ramin Ekhteiari; Durdagi, Serdar; Pereira, Catarina; Varela, J.; Barreira, Luísa; Custódio, Luísa
    Different Polygonum species have in vitro neuroprotective properties and are traditionally used for their anti-ageing benefits. In this context this work explored for the first time P. maritimum (sea knotgrass) as a potential source of natural products with industrial application as cognitive enhancers with anti-ageing potential. For that purpose methanol and dichloromethane extracts were prepared from leaves and roots of that species and evaluated for in vitro neuroprotective effects through the inhibition on acetyl- (AChE) and butyrylcholinesterase (BuChE), protection against hydrogen peroxide (H2O2)-induced cytoxicity on neuroblastoma cells and lipopolysaccharide (LPS)-induced neuroinflammation on microglia cells. The extracts were chemically characterized by...

  3. The P-type ATPase inhibiting potential of polyoxotungstates.

    Gumerova, Nadiia; Krivosudský, Lukáš; Fraqueza, Gil; Breibeck, Joscha; Al-Sayed, Emir; Tanuhadi, Elias; Bijelic, Aleksandar; Fuentes, Juan; Aureliano, M.; Rompel, Annette
    Polyoxometalates (POMs) are transition metal complexes that exhibit a broad diversity of structures and properties rendering them promising for biological purposes. POMs are able to inhibit a series of biologically important enzymes, including phosphatases, and thus are able to affect many biochemical processes. In the present study, we analyzed and compared the inhibitory effects of nine different polyoxotungstates (POTs) on two P-type ATPases, Ca2+-ATPase from skeletal muscle and Na+/K+-ATPase from basal membrane of skin epithelia. For Ca2+-ATPase inhibition, an in vitro study was performed and the strongest inhibitors were determined to be the large heteropolytungstate K9(C2H8N)5[H10Se2W29O103] (Se2W29) and the Dawson-type...

  4. Membrane-enriched proteome changes and prion protein expression during neural differentiation and in neuroblastoma cells

    Macedo, J. A.; Schrama, D.; Duarte, I.; Tavares, E.; Renaut, J.; Futschik, M. E.; Rodrigues, P. M.; Melo, E. P.
    Background The function of the prion protein, involved in the so-called prion diseases, remains a subject of intense debate and the possibility that it works as a pleiotropic protein through the interaction with multiple membrane proteins is somehow supported by recent reports. Therefore, the use of proteomic and bioinformatics combined to uncover cellular processes occurring together with changes in the expression of the prion protein may provide further insight into the putative pleiotropic role of the prion protein. Results This study assessed the membrane-enriched proteome changes accompanying alterations in the expression of the prion protein. A 2D-DIGE approach...

  5. Cryptic diversity, geographical endemism and allopolyploidy in NE Pacific seaweeds

    Neiva, João; Serrão, Ester; Anderson, Laura; Raimondi, Peter T.; Martins, Neusa; Gouveia, Licínia; Paulino, Cristina; Coelho, Nelson C.; Miller, Kathy A.; Reed, Daniel C.; Ladah, Lydia B.; Pearson, Gareth A.
    Background Molecular markers are revealing a much more diverse and evolutionarily complex picture of marine biodiversity than previously anticipated. Cryptic and/or endemic marine species are continually being found throughout the world oceans, predominantly in inconspicuous tropical groups but also in larger, canopy-forming taxa from well studied temperate regions. Interspecific hybridization has also been found to be prevalent in many marine groups, for instance within dense congeneric assemblages, with introgressive gene-flow being the most common outcome. Here, using a congeneric phylogeographic approach, we investigated two monotypic and geographically complementary sister genera of north-east Pacific intertidal seaweeds (Hesperophycus and Pelvetiopsis), for which...

  6. Evolution of the angiopoietin-like gene family in teleosts and their role in skin regeneration

    Costa, Rita A.; Cardoso, João C. R.; Power, Deborah
    Background The skin in vertebrates is a protective barrier and damage is rapidly repaired to re-establish barrier function and maintain internal homeostasis. The angiopoietin-like (ANGPTL) proteins are a family of eight secreted glycoproteins with an important role in skin repair and angiogenesis in humans. In other vertebrates their existence and role in skin remains largely unstudied. The present study characterizes for the first time the homologues of human ANGPTLs in fish and identifies the candidates that share a conserved role in skin repair using a regenerating teleost skin model over a 4-day healing period. Results Homologues of human...

  7. A genome-wide transcriptome map of pistachio (Pistacia vera L.) provides novel insights into salinity-related genes and marker discovery

    Moazzzam Jazi, Maryam; Seyedi, Seyed M.; Ebrahimie, Esmaeil; Ebrahimi, Mansour; De Moro, Gianluca; Botanga, Christopher
    Background Pistachio (Pistacia vera L.) is one of the most important commercial nut crops worldwide. It is a salt-tolerant and long-lived tree, with the largest cultivation area in Iran. Climate change and subsequent increased soil salt content have adversely affected the pistachio yield in recent years. However, the lack of genomic/global transcriptomic sequences on P. vera impedes comprehensive researches at the molecular level. Hence, whole transcriptome sequencing is required to gain insight into functional genes and pathways in response to salt stress. Results RNA sequencing of a pooled sample representing 24 different tissues of two pistachio cultivars with contrasting salinity...

  8. Contrasting movements and residency of two serranids in a small Macaronesian MPA

    Afonso, Pedro; Abecasis, David; Santos, Ricardo Serrão; Fontes, Jorge
    Protection inside marine reserves and the desired ‘reserve effect’ depend directly on the home rangeand sedentary behaviour of fishes throughout their lives. Benthic territorial predators are typically seenas major candidates to beneficiaries, but empirical evidence of short-versus long term residency is stillsurprisingly rare. We studied the inter-annual patterns of site fidelity and finer scale movements in twoof the most abundant benthic predators of the NE Atlantic coastal reefs, the dusky grouper (Epinephelusmarginatus) and the blacktail comber (Serranus atricauda), using passive acoustic telemetry inside andaround a small marine reserve in the Azores islands, central North Atlantic. Individuals of both speciesresided in...

  9. Contrasting movements and residency of two serranids in a small Macaronesian MPA

    Afonso, P.; Abecasis, D.; Santos, Ricardo Serrão; Fontes, Jorge
    Protection inside marine reserves and the desired ‘reserve effect’ depend directly on the home rangeand sedentary behaviour of fishes throughout their lives. Benthic territorial predators are typically seenas major candidates to beneficiaries, but empirical evidence of short-versus long term residency is stillsurprisingly rare. We studied the inter-annual patterns of site fidelity and finer scale movements in twoof the most abundant benthic predators of the NE Atlantic coastal reefs, the dusky grouper (Epinephelusmarginatus) and the blacktail comber (Serranus atricauda), using passive acoustic telemetry inside andaround a small marine reserve in the Azores islands, central North Atlantic. Individuals of both speciesresided in...

  10. A well-kept treasure at depth: precious red coral rediscovered in Atlantic deep coral gardens (SW Portugal) after 300 years

    Boavida, Joana; Paulo, Diogo; Aurelle, Didier; Arnaud-Haond, Sophie; Marschal, Christian; Reed, John; Gonçalves, J. M. S.; Serrão, Ester
    The highly valuable red coral Corallium rubrum is listed in several Mediterranean Conventions for species protection and management since the 1980s. Yet, the lack of data about its Atlantic distribution has hindered its protection there. This culminated in the recent discovery of poaching activities harvesting tens of kg of coral per day from deep rocky reefs off SW Portugal. Red coral was irregularly exploited in Portugal between the 1200s and 1700s, until the fishery collapsed. Its occurrence has not been reported for the last 300 years.

  11. A new Euthria (Gastropoda: Buccinidae) from the Cape Verde Archipelago

    Fraussen, Koen; Afonso, Carlos M. L.
    A small, yet beautiful species collected at north to northwestern Sal Island (Cape Verde Archipelago), and hitherto consistently identified as E. calderoni Rolán, 1985, is recognised as distinct and is described as new: Euthria taeniopsoides sp. nov.

  12. Morphological analysis and description of the ovaries of female silky sharks, Carcharhinus falciformis (Müller & Henle, 1839)

    Rego, Mariana G.; Hazin, Fabio H. V.; Evencio Neto, Joaquim; Oliveira, P. G. V.; Soares, Maria Goretti; Torres, Keilla Regina L. S.; Lana, Fernanda O.; Roque, Pollyana C. G.; Santos, Natalia L.; Coelho, Rui
    This work aims to study the female reproductive tract of silky sharks, Carcharhinus falciformis, captured in the South and Equatorial Atlantic Ocean. Samples were collected between January 2008 and March 2010 through oceanic commercial vessels that targeted tuna and swordfish, with a total of 17 females collected. The methodologies followed for analyzing the ovaries of those females included both macroscopic and histological analysis. Macroscopically, it was possible to determine that the ovaries on these sharks is suspended by mesenteries in the anterior section of the body cavity, heavily irrigated by blood vessels, and contains a wide range of oocytes. Ovaries were found in three distinct...

  13. Use of encounter data to model spatio-temporal distribution patterns of endangered smalltooth sawfish, Pristis pectinata, in the western Atlantic

    Waters, John D.; Coelho, Rui; Fernandez-Carvalho, Joana; Timmers, Amy A.; Wiley, Tonya; Seitz, Jason C.; Mcdavitt, Matthew T.; Burgess, George H.; Poulakis, Gregg R.
    1. Sawfishes are among themost threatened fish species globally,with only the smalltooth sawfish (Pristis pectinata) currently regularly observed in the westernAtlantic. TheNational Sawfish EncounterDatabase (NSED) documents reported encounters with sawfishes in the western Atlantic and contains 4945 reports of 8773 individual P. pectinata (1782–2011). 2. Statistical modelling (generalized linear models and generalized additive models) and kernel density analyses were used to (1) identify spatio-temporal patterns among encounter reports, including range reduction in the western Atlantic; (2) determine current distribution to identify areas and time periods where conservation and recovery efforts could be focused; and (3) identify and describe spatio-temporal distribution patterns of large juveniles and adults. 3....

  14. Identification of weakfish Cynoscion (Gill) in the Bay of Panama with RFLP markers

    Vergara-Chen, Carlos; González-Wangüemert, Mercedes; Bermingham, Eldredge; D’Croz, L.
    A molecular approach, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), was developed to identify the planktonic larvae of Cynoscion species. Species-specific mitochondrial DNA markers were developed using three restriction endonucleases (DdeI, HaeIII and HinfI). These markers permitted the accurate discrimination of the five Cynoscion species in the Bay of Panama.

  15. A mitochondrial DNA based phylogeny of weakfish species of the Cynoscion group (Pisces: Sciaenidae)

    Vergara-Chen, Carlos; Aguirre, Windsor E.; González-Wangüemert, Mercedes; Bermingham, Eldredge
    We infer the phylogeny of fishes in the New World Cynoscion group (Cynoscion, Isopisthus, Macrodon, Atractoscion, Plagioscion) using 1603bp of DNA sequence data from three mitochondrial genes. With the exception of Plagioscion, whose position was ambiguous, the Cynoscion group is monophyletic. However, several genera examined are not monophyletic. Atlantic and Pacific species of Cynoscion are interspersed in the tree and geminate species pairs are identified. Intergeneric relationships in the group are clarified. Our analysis is the first comprehensive phylogeny for the Cynoscion group based on molecular data and provides a baseline for future comparative studies of this important group.

  16. High gene flow promotes the genetic homogeneity of the fish goby Pomatoschistus marmoratus (Risso, 1810) from Mar Menor coastal lagoon and adjacent marine waters (Spain)

    Vergara-Chen, Carlos; González-Wangüemert, Mercedes; Marcos, Concepción; Pérez-Ruzafa, Ángel
    The extreme environmental variability of coastal lagoons suggests that physical and ecological factors could contribute to the genetic divergence among populations occurring in lagoon and open-coast environments. In this study we analysed the genetic variability of lagoon and marine samples of the sand goby, Pomatoschistus marmoratus (Risso, 1810) (Pisces: Gobiidae), on the SW Spain coast. A fragment of mitochondrial DNA control region (570 bp) was sequenced for 196 individuals collected in five localities: Lo Pagan, Los Urrutias and Playa Honda (Mar Menor coastal lagoon), and Veneziola and Mazarro´n (Mediterranean Sea). The total haplotype diversity was h = 0.9424 ± 0.0229, and the total nucleotide diversity was p = 0.0108...

  17. First report of the nutritional profile and antioxidant potential of Holothuria arguinensis, a new resource for aquaculture in Europe

    Roggatz, Christina C.; González-Wangüemert, Mercedes; Pereira, Hugo; Rodrigues, Maria João; Silva, Manuela Moreira da; Barreira, Luísa; Varela, João; Custódio, Luísa
    This work reports for the first time the nutritional profile and antioxidant potential of the edible sea cucumber Holothuria arguinensis from the North-eastern Atlantic. H. arguinensis has high levels of protein, with the amino acids profile dominated by alanine, glycine and proline and low lysine/arginine ratios. Its carbohydrate and energetic contents are also low as well as the total lipid levels, although its lipid profile is rich in polyunsaturated fatty acids (PUFA), especially arachidonic, eicosapentaenoic and docosahexaenoic acids. In addition, H. arguinensis has high levels of calcium. The water and ethanol extracts show ability to scavenge free radicals and to...

  18. A new record of Holothuria arguinensis colonizing the Mediterranean Sea

    González-Wangüemert, Mercedes; Borrero-Pérez, Giomar
    Holothuria arguinensis is reported for the first time on the south-eastern Spanish coast. One specimen of this species was identified in El Mojo´n (Alicante, Spain, 37850′54.17′′N 0845′39.90′′W). This finding widens the geographical range of H. arguinensis and demonstrates that its environmental requirements are changing. Future genetic studies in this species would allow us to improve our understanding of its colonization from the Atlantic Ocean to the Mediterranean Sea.

  19. Modelling spatial and temporal scales for spill-over and biomass exportation from MPAs and their potential for fisheries enhancement

    Pérez-Ruzafa, Ángel; Martín, Ernesto; Marcos, Concepción; Zamarro, José Miguel; Stobart, Ben; Harmelin-Vivien, Mireille; Polti, Sandrine; Planes, Serge; García-Charton, José Antonio; González-Wangüemert, Mercedes
    Marine protected areas (MPAs) are considered as a tool for marine conservation and sustainable fishery resource management. Improvements in fishery yields should take place via the spill-over of individuals from the reserve. In general, it has been demonstrated that MPAs affect the density and biomass of the organisms within them, however, little evidence has been found in order to assess the exportation of individuals across their boundaries. In this study, a simple model involving population growth, harvest, and the diffusion coefficient for individuals was used to explore the effects of protection on populations inside the reserve and the spill-over of...

  20. Effects of fishing protection on the genetic structure of fish populations

    Pérez-Ruzafa, Ángel; González-Wangüemert, Mercedes; Lenfant, Philippe; Marcos, Concepción; García-Charton, José Antonio
    Marine reserves have been identified as an important tool in the management of fishery resources and their number is increasing rapidly, most of them being on islands. However, knowledge on the real effect of protection from fishing on the genetic structure of populations, the spatial scales involved, or the suitability of islands as reserves in terms of connectivity, is scarce. This paper analyses the effects of fishery protection on the genetic structure of populations of Diplodus sargus, a target species, in protected and non-protected areas of the western Mediterranean. Populations studied showed high genetic variability at spatial scales from 101 to 103 km. Protected areas have significantly...

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