Mostrando recursos 1 - 17 de 17

  1. Analysis of opo cis-regulatory landscape uncovers Vsx2 requirement in early eye morphogenesis

    Gago-Rodrigues, Inês; Fernández-Miñán, Ana; Letelier, Joaquin; Naranjo, Silvia; Tena, Juan J.; Gómez-Skarmeta, José L.; Martinez-Morales, Juan R.
    The self-organized morphogenesis of the vertebrate optic cup entails coupling the activation of the retinal gene regulatory network to the constriction-driven infolding of the retinal epithelium. Yet the genetic mechanisms underlying this coordination remain largely unexplored. Through phylogenetic footprinting and transgenesis in zebrafish, here we examine the cis-regulatory landscape of opo, an endocytosis regulator essential for eye morphogenesis. Among the different conserved enhancers identified, we isolate a single retina-specific element (H6_10137) and show that its activity depends on binding sites for the retinal determinant Vsx2. Gain- and loss-of-function experiments and ChIP analyses reveal that Vsx2 regulates opo expression through direct...

  2. Numb/Numbl-Opo antagonism controls retinal epithelium morphogenesis by regulating integrin endocytosis

    Bogdanović, Ozren; Delfino-Machín, Mariana; Nicolás-Pérez, María; Gavilán, María P.; Gago-Rodrigues, Inês; Fernández-Miñán, Ana; Lillo, Concepción; Ríos, Rosa M.; Wittbrodt, Joachim; Martínez-Morales, Juan R.
    Polarized trafficking of adhesion receptors plays a pivotal role in controlling cellular behavior during morphogenesis. Particularly, clathrin-dependent endocytosis of integrins has long been acknowledged as essential for cell migration. However, little is known about the contribution of integrin trafficking to epithelial tissue morphogenesis. Here we show how the transmembrane protein Opo, previously described for its essential role during optic cup folding, plays a fundamental role in this process. Through interaction with the PTB domain of the clathrin adaptors Numb and Numbl via an integrin-like NPxF motif, Opo antagonizes Numb/Numbl function and acts as a negative regulator of integrin endocytosis in...

  3. AB1258­HPR pilot study: does a mediterranean diet­based weight loss programme improve health outcomes in overweight older women with knee osteoarthritis?

    Caixinha, Mónica; Mateus, Maria Palma; Pais, Sandra; Pinto, Ezequiel
    Osteoarthritis (OA) is one of the leading causes of pain and disability among older adults, particularly women. Pain and mobility disability are major consequences of knee OA which and can interfere with the functional autonomy of elderly and thus, making it difficult to perform activities of daily living. Evidence suggests that obesity is strongly linked to knee OA and that non­pharmacological therapy should be based on physical activity and weight loss in case of overweight and obesity. A positive relationship between adherence to the Mediterranean diet and health outcomes has been widely discussed in scientific literature, including its potential benefits in weight loss.

  4. Avaliação da dose em recém-nascidos numa unidade neonatal

    Ribeiro, Luís; Rodrigues, Sónia; Sousa, Patrick; Abrantes, António; Sousa, Liliana; Almeida, Rui; Azevedo, Kevin; Pinheiro, João
    Objetivo: Avaliar a dose a que estão sujeitos os recém-nascidos internados numa Unidade de Cuidados Intensivos Neonatais (UCIN) durante a realização de radiografias em incidência AP ao tórax e comparar os valores obtidos da Dose à Entrada da Pele (DEP), tanto com os recomendados internacionalmente para os recém-nascidos como com os resultados de estudos anteriores. Pretende-se também verificar se as doses utilizadas se encontram abaixo dos valores recomendados e se os pacientes se apresentam ou não em risco face às radiações ionizantes a que foram submetidos. Metodologia: Foi utilizado um equipamento de radiologia convencional móvel Siemens Mobilett XP Hybrid, uma...

  5. Radiographers perceptions of magnetic resonance imaging: a study of the causes that lead to the exams repetition

    Filipe, Tiago; Ribeiro, Luís; Almeida, Rui; Rodrigues, Sónia; Azevedo, Kevin; Silva, Carlos; Abrantes, António
    Purpose: To know how often occur the repetitions of MRI exams and sequences in radiology departments. Methods and Materials: A self applied-questionnaire was used as instrument and assigned to 57 radiographers who performed MRI exams to determine which were the causes that lead to the repetition. The questionnaires were interpreted and statistically analyzed through descriptive statistics and Spearman’s rho correlations. Results: At a 95% confidence interval, the major results suggest that the patient’s movement during de MRI exams is the main cause to repeat this exams (mean of 3.88 on a 5 points likert scale). However, there are causes related to the...

  6. Global geriatric evaluation of the Algarve population

    Braz, Nídia; Pais, Sandra; Cordeiro, Clara; Oliveira, Giovanna; Conde, Monserrat
    The ageing of population challenges communities to adapt and evolve to accommodate the needs of people that live longer (mostly out of work, either healthy, fragile or with chronic disease). Population ageing in the Algarve is higher than in overall Portugal. Studies on health conditions, frailty risk factors and elderly specific needs are undeveloped in Portugal and unknown in the Algarve. Objective To prepare a tool for Global Geriatric Evaluation, to be used in the “Survey of Health and Ageing in the Region of Algarve - SHARA”, a commitment to “European Innovation Partnership on Active and Healthy Ageing”. Methods A preliminary version of the screening...

  7. Isolation and characterization of piscine osteonectin and downregulation of Its expression by PTH-related protein

    Redruello, Begoña; Estêvão, M. Dulce; Rotllant, J.; Guerreiro, P. M.; Anjos, Liliana; Canario, Adelino V. M.; Power, Deborah
    The skeleton is the main source of osteonectin mRNA in adults of the seawater teleost sea bream Sparus auratus. It is expressed by cells forming the basement membrane of calcifying tissue indicating that, as in mammals, it may play a role in osteoblast differentiation. PTHrP induced downregulation of osteonectin mRNA in vitro in scales, a mineralizing tissue with bone-like metabolism. This indicates a means to redirect calcium to activities such as vitellogenesis when this ion is in high demand.

  8. Immunohistochemical detection of estrogen receptors in fish scales

    Pinto, Patricia; Estêvão, M. Dulce; Redruello, Begoña; Socorro, S.; Canario, Adelino V. M.; Power, Deborah
    Calcium mobilization from internal stores, such as scales, induced by 17b-estradiol during sexual maturation in salmonids is well documented. This calcium mobilization from scales is proposed to be mediated by the estrogen receptor (ER). However, the ER subtypes involved and signaling mechanisms responsible for this effect remain to be fully characterized. In the present study, we have localized ERa, ERba and ERbb proteins in juvenile and adult sea bream (Sparus auratus) and Mozambique tilapia (Oreochromis mossambicus) scales by immunohistochemistry with sea bream ER subtype specific antibodies. The three ERs were detected in isolated or small groups of round cells, in the basal layer...

  9. Cellular morphology and markers of cartilage and bone in the marine teleost Sparus auratus

    Estêvão, M. Dulce; Silva, Nadia; Redruello, Begoña; Costa, Rita; Gregório, Sílvia F.; Canario, Adelino V. M.; Power, Deborah
    Modifications have been characterised in terms of cellular organisation and the extracellular matrix (ECM) during bone ontogeny in the sea bream (Sparus auratus). During endochondral development, the agglomeration of matrix-secreting cells gives rise to chondrones; these chondrones frequently contain proliferating-cell-nuclearantigen-positive cells, which subsequently become large collagen-II-positive cells with the characteristics of chondrocytes. Moreover, the matrix:cell ratio within the perichondrium increases, accompanied by a modification in ECM composition. Mineralisation of cartilage ECM is marked by a rapid fall in cell number, the switching off of collagen II transcription and the switching on of collagen X transcription, followed by collagen I transcription and...

  10. Manual da qualidade e abordagem por processos: o caso de uma sala de mamografia

    Almeida, Rui; Silva, Carlos; Fernandes, Ana; Abrantes, António; Ribeiro, Luís; Azevedo, Kevin
    Publicação integrada na produção editorial do Centro de Investigação UNIDE (Unidade de Investigação em Desenvolvimento Empresarial), ISCTE business School do Instituto Universitário de Lisboa.

  11. The evaluation of patient’s anxiety levels undergoing magnetic resonance imaging examination

    Medeiros, Filipa; Azevedo, Kevin; Ribeiro, Luís; Abrantes, António; Rodrigues, Sónia; Silva, Carlos; Almeida, Rui
    Purpose: To analyse possible differences between the anxiety levels of patients who receive additional information about the MRI scan, and patients who receive only basic instructions from the hospital, and to verify if there is variation in anxiety levels of patients before and after the examination, and relate them to gender, age, education level, and previous MRI examinations. Methods and Materials: The total sample of 60 patients was divided into a control group and an experimental group, and each one divided into two subgroups before and after the exam, and then it was applied STAI form Y-1 to both, after the sociodemographic questions. Results: At...

  12. Study of the scattered x-ray distributions around a mammography unit

    Lima, Patricia; Sousa, Patrick Emmanuel; Rodrigues, Sónia; Abrantes, António; Silva, Carlos; Ribeiro, Luís; Almeida, Rui; Azevedo, Kevin
    Purpose: To measure the distribution of scattered radiation in a mammography unit for shielding design purposes. Methods and Materials: Measurements were carried out with an anthropomorphic phantom used to simulate a body from one adult patient. The dose rate for scattered radiation was recorded by placing a solid state sensor (Unfors Xi Survey Detector) every 10 degrees at 1 m distance from the FOV centre, in the axial and coronal planes of the breast. The scattered radiation distribution was treated as isodose curves, specifying the amount of radiation that will be delivered to a specific point in the room around the equipment...

  13. Qualidade em radiologia: análise da percepção da qualidade e satisfação em dois serviços de radiologia distintos, segundo os utentes da consulta externa

    Almeida, Rui; Silva, Carlos; Ribeiro, Luís; Abrantes, António; José, Helena; Ribeiro, Anabela
    A elaboração deste trabalho deveu-se à necessidade de avaliar a qualidade percebida e a satisfação dos utentes/doentes de dois serviços de Radiologia distintos, sendo um deles certificados pelas normas ISO 9001:2000 e outro não certificado. O principal objectivo consistiu em avaliar o desempenho desses serviços de Radiologia e verificar se, na prática, um serviço com o sistema de gestão da qualidade (SGQ) implementado, se traduzia numa maior qualidade e satisfação dos serviços prestados, na perspectiva dos utentes/doentes. É um estudo quantitativo de natureza comparativa e descritivo-correlacional e a metodologia utilizada teve por base um questionário dirigido aos utentes da Consulta Externa baseado numa escala...

  14. Effect of informed consent for administration of intravenous contrast material in the anxiety level of patients submitted to computed tomography exams

    Azevedo, Kevin; Abrantes, António; Ribeiro, Luís; Almeida, Rui; Silva, Carlos
    Purpose: Evaluation and relation of the state and trait anxiety level in patients before the delivery of informed consent with patients after that, and the evaluation of eventual relations between psychometric tests and sociodemographic characteristics. Methods and Materials: The present study is quasi-experimental. The total sample of 161 patients was divided in a control group and an experimental group, and then state-trait anxiety inventory was applied to both, after the sociodemographic interview and always after the consent of participation of the patient. Results: At a 95 % confidence interval, the intergroup difference between means of state anxiety ranged from -0.929 and -0.810. The value of...

  15. Patient´s knowledge about radiation and radiological protection

    Pinheiro, João; Ribeiro, Luís; Rodrigues, Sónia; Abrantes, António; Silva, Carlos; Almeida, Rui
    Purpose: To obtain and analyse patient´s knowledge and perceptions regarding radiation exposure, from both natural and man-made radiation of medical procedures and interventions. Verify if patients worry about their exposure when undergoing medical exams, are aware of associated risks and means of radiological protection and if their knowledge on medical radiation exposure affects their own decisions. Methods and Materials: On a medical environment a self-applied questionnaire was used as instrument and assigned to patients who would undergo medical imaging exams involving ionising radiation. A total of 300 valid questionnaires were interpreted and statistically analysed through descriptive statistics and Phi & Cramer´s V correlation tests. Results: 44.3%...

  16. UAN: underwater acoustic network

    Caiti, A.; Felisberto, P.; Husoy, T.; Jesus, S. M.; Karasalo, I.; Massimelli, R.; Reinen, Tor Arne; Silva, A.
    Acoustic networks are for underwater what wifi is for terrestrial networks. The ocean is a nearly perfect media for acoustic waves in which regards long range propagation but poses a number of challenges in terms of available bandwidth, Doppler spread and channel fading. These limitations originate in the physical properties of the ocean, namely its anisotropy and boundary interaction which are particularly relevant in coastal waters where acoustic propagation becomes predominantly de- pendent on seafloor and sea surface properties. The acoustic communication channel is therefore multipath dominated and time and Doppler spread variable. The problem is aggravated when involving moving...

  17. O azeite na alimentação humana

    Lopes, Maria Palma Mateus; Estêvão, M. Dulce
    O azeite virgem é o sumo resultante da azeitona (fruto da Oliveira, Olea Europea L.) que conserva o sabor, aroma e todas as propriedades do fruto do qual tem origem, pelo que, possui características organolépticas particulares, colocando-o num lugar cimeiro entre as gorduras (Cenzano et al., 1988; March, 1994). É, além disso, a única gordura vegetal que pode ser consumida directamente, virgem e crua (Gonçalves Ferreira, 1994; March, 1994). O seu valor calórico é de 9 Kcal/g, a mesma proporção de qualquer outra gordura, animal ou vegetal (Gonçalves Ferreira, 1994; Peres, 1994).

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