Recursos de colección

ARCA - Access to Research and Communication Annals - IGC Repository (853 recursos)

The Institutional Repository of Instituto Gulbenkian de Ciência, developed under the project RCAAP, is intended to collect, preserve and promote open access to the intellectual production of their researchers.

Lymphocyte Physiology

Mostrando recursos 1 - 20 de 38

  1. Escherichia coliadaptation to the gut environment: a constant fight for survival

    Gordo, Isabel; Demengeot, Jocelyne; Xavier, Karina
    LAO/ITQB, FCT.

  2. Adaptive immunity increases the pace and predictability of evolutionary change in commensal gut bacteria

    Barroso-Batista, João; Demengeot, Jocelyne; Gordo, Isabel
    Co-evolution between the mammalian immune system and the gut microbiota is believed to have shaped the microbiota's astonishing diversity. Here we test the corollary hypothesis that the adaptive immune system, directly or indirectly, influences the evolution of commensal species. We compare the evolution of Escherichia coli upon colonization of the gut of wild-type and Rag2(-/-) mice, which lack lymphocytes. We show that bacterial adaptation is slower in immune-compromised animals, a phenomenon explained by differences in the action of natural selection within each host. Emerging mutations exhibit strong beneficial effects in healthy hosts but substantial antagonistic pleiotropy in immune-deficient mice. This...

  3. A Novel Quantitative Fluorescent Reporter Assay for RAG Targets and RAG Activity

    Trancoso, Inês; Bonnet, Marie; Gardner, Rui; Carneiro, Jorge; Barreto, Vasco M.; Demengeot, Jocelyne; Sarmento, Leonor M.
    Recombination-Activating Genes (RAG) 1 and 2 form the site specific recombinase that mediates V(D)J recombination, a process of DNA editing required for lymphocyte development and responsible for their diverse repertoire of antigen receptors. Mistargeted RAG activity associates with genome alteration and is responsible for various lymphoid tumors. Moreover several non-lymphoid tumors express RAG ectopically. A practical and powerful tool to perform quantitative assessment of RAG activity and to score putative RAG-Recognition signal sequences (RSS) is required in the fields of immunology, oncology, gene therapy, and development. Here we report the detailed characterization of a novel fluorescence-based reporter of RAG activity,...

  4. INFRAFRONTIER--providing mutant mouse resources as research tools for the international scientific community

    Meehan, T.F.; Demengeot, Jocelyne; 44 colaborators, Infrafrontier Consortium
    The laboratory mouse is a key model organism to investigate mechanism and therapeutics of human disease. The number of targeted genetic mouse models of disease is growing rapidly due to high-throughput production strategies employed by the International Mouse Phenotyping Consortium (IMPC) and the development of new, more efficient genome engineering techniques such as CRISPR based systems. We have previously described the European Mouse Mutant Archive (EMMA) resource and how this international infrastructure provides archiving and distribution worldwide for mutant mouse strains. EMMA has since evolved into INFRAFRONTIER (http://www.infrafrontier.eu), the pan-European research infrastructure for the systemic phenotyping, archiving and distribution of...

  5. INFRAFRONTIER--providing mutant mouse resources as research tools for the international scientific community

    Meehan, T.F.; Demengeot, Jocelyne; 44 colaborators, Infrafrontier Consortium
    The laboratory mouse is a key model organism to investigate mechanism and therapeutics of human disease. The number of targeted genetic mouse models of disease is growing rapidly due to high-throughput production strategies employed by the International Mouse Phenotyping Consortium (IMPC) and the development of new, more efficient genome engineering techniques such as CRISPR based systems. We have previously described the European Mouse Mutant Archive (EMMA) resource and how this international infrastructure provides archiving and distribution worldwide for mutant mouse strains. EMMA has since evolved into INFRAFRONTIER (http://www.infrafrontier.eu), the pan-European research infrastructure for the systemic phenotyping, archiving and distribution of...

  6. INFRAFRONTIER--providing mutant mouse resources as research tools for the international scientific community

    Meehan, T.F.; Demengeot, Jocelyne; 44 colaborators, Infrafrontier Consortium
    This deposit is composed by a publication in which the IGC' authors have had the role of collaboration (it's a collaboration publication). This type of deposit in ARCA is in restrictedAccess (it can't be in open access to the public), and could only be accessed by two ways: either by requesting a legal copy to the author (the email contact present in this deposit) or by visiting the following link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383977/

  7. The first steps of adaptation of Escherichia coli to the gut are dominated by soft sweeps

    João Barroso-Batista; Ana Sousa; Marta Lourenço; Marie-Louise Bergman; Jocelyne Demengeot; Karina B. Xavier; Isabel Gordo
    The accumulation of adaptive mutations is essential for survival in novel environments. However, in clonal populations with a high mutational supply, the power of natural selection is expected to be limited. This is due to clonal interference - the competition of clones carrying different beneficial mutations - which leads to the loss of many small effect mutations and fixation of large effect ones. If interference is abundant, then mechanisms for horizontal transfer of genes, which allow the immediate combination of beneficial alleles in a single background, are expected to evolve. However, the relevance of interference in natural complex environments, such as the gut, is poorly known. To address this...

  8. Tolerogenic insulin peptide therapy precipitates type 1 diabetes

    Bergman, Marie-Louise; Lopes-Carvalho, Thiago; Martins, Ana-Catarina; Grieco, Fabio A.; Eizirik, Décio L.; Demengeot, Jocelyne
    The deposited article version contains attached the supplementary materials within the pdf.

  9. Tolerogenic insulin peptide therapy precipitates type 1 diabetes

    Bergman, Marie-Louise; Lopes-Carvalho, Thiago; Martins, Ana-Catarina; Grieco, Fabio A.; Eizirik, Décio L.; Demengeot, Jocelyne
    This deposit is composed by the main article plus the supplementary materials of the publication.

  10. Fatal CTLA-4 heterozygosity with autoimmunity and recurrent infections: a de novo mutation

    Moraes-Fontes, Maria Francisca; Hsu, Amy P.; Caramalho, Iris; Martins, Catarina; Araújo, Ana Carolina; Lourenço, Filipa; Taulaigo, Anna V.; Lladó, Ana; Holland, Steven M.; Uzel, Gulbu
    Further funders are not indicated in the document. There is no public supplementary material available for this publication. This deposit is composed by the main article, and it hasn't any supplementary materials associated.

  11. Fatal CTLA-4 heterozygosity with autoimmunity and recurrent infections: a de novo mutation

    Moraes-Fontes, Maria Francisca; Hsu, Amy P.; Caramalho, Iris; Martins, Catarina; Araújo, Ana Carolina; Lourenço, Filipa; Taulaigo, Anna V.; Lladó, Ana; Holland, Steven M.; Uzel, Gulbu
    Further funders are not indicated in the document. There is no public supplementary material available for this publication. This deposit is composed by the main article, and it hasn't any supplementary materials associated.

  12. iRAGu: A Novel Inducible and Reversible Mouse Model for Ubiquitous Recombinase Activity

    Bonnet, Marie; Sarmento, Leonor Morais; Martins, Ana C.; Sobral, Daniel; Silva, Joana; Demengeot, Jocelyne
    This deposit is composed by the main article plus the supplementary materials of the publication.

  13. iRAGu: A Novel Inducible and Reversible Mouse Model for Ubiquitous Recombinase Activity

    Bonnet, Marie; Sarmento, Leonor Morais; Martins, Ana C.; Sobral, Daniel; Silva, Joana; Demengeot, Jocelyne
    This deposit is composed by the main article plus the supplementary materials of the publication.

  14. RAG Recombinase as a Selective Pressure for Genome Evolution

    Passagem-Santos, D.; Bonnet, M.; Sobral, D.; Trancoso, I.; Silva, J.G.; Barreto, V.M.; Athanasiadis, A.; Demengeot, J.; Pereira-Leal, J.B.
    The RAG recombinase is a domesticated transposable element co-opted in jawed vertebrates to drive the process of the so-called V(D)J recombination, which is the hallmark of the adaptive immune system to produce antigen receptors. RAG targets, namely, the Recombination Signal Sequences (RSS), are rather long and degenerated sequences, which highlights the ability of the recombinase to interact with a wide range of target sequences, including outside of antigen receptor loci. The recognition of such cryptic targets by the recombinase threatens genome integrity by promoting aberrant DNA recombination, as observed in lymphoid malignancies. Genomes evolution resulting from RAG acquisition is an...

  15. RAG Recombinase as a Selective Pressure for Genome Evolution

    Passagem-Santos, D.; Bonnet, M.; Sobral, D.; Trancoso, I.; Silva, J.G.; Barreto, V.M.; Athanasiadis, A.; Demengeot, J.; Pereira-Leal, J.B.
    The RAG recombinase is a domesticated transposable element co-opted in jawed vertebrates to drive the process of the so-called V(D)J recombination, which is the hallmark of the adaptive immune system to produce antigen receptors. RAG targets, namely, the Recombination Signal Sequences (RSS), are rather long and degenerated sequences, which highlights the ability of the recombinase to interact with a wide range of target sequences, including outside of antigen receptor loci. The recognition of such cryptic targets by the recombinase threatens genome integrity by promoting aberrant DNA recombination, as observed in lymphoid malignancies. Genomes evolution resulting from RAG acquisition is an...

  16. Regulatory T cells control strain specific resistance to Experimental Autoimmune Prostatitis

    Breser, Maria L.; Lino, Andreia C.; Motrich, Ruben D.; Godoy, Gloria J.; Demengeot, Jocelyne; Rivero, Virginia E.
    Susceptibility to autoimmune diseases results from the encounter of a complex and long evolved genetic context with a no less complex and changing environment. Major actors in maintaining health are regulatory T cells (Treg) that primarily dampen a large subset of autoreactive lymphocytes escaping thymic negative selection. Here, we directly asked whether Treg participate in defining susceptibility and resistance to Experimental Autoimmune Prostatitis (EAP). We analyzed three common laboratory strains of mice presenting with different susceptibility to autoimmune prostatitis upon immunization with prostate proteins. The NOD, the C57BL/6 and the BALB/c mice that can be classified along a disease score...

  17. Regulatory T cells control strain specific resistance to Experimental Autoimmune Prostatitis

    Breser, Maria L.; Lino, Andreia C.; Motrich, Ruben D.; Godoy, Gloria J.; Demengeot, Jocelyne; Rivero, Virginia E.
    Susceptibility to autoimmune diseases results from the encounter of a complex and long evolved genetic context with a no less complex and changing environment. Major actors in maintaining health are regulatory T cells (Treg) that primarily dampen a large subset of autoreactive lymphocytes escaping thymic negative selection. Here, we directly asked whether Treg participate in defining susceptibility and resistance to Experimental Autoimmune Prostatitis (EAP). We analyzed three common laboratory strains of mice presenting with different susceptibility to autoimmune prostatitis upon immunization with prostate proteins. The NOD, the C57BL/6 and the BALB/c mice that can be classified along a disease score...

  18. Regulatory T cells control strain specific resistance to Experimental Autoimmune Prostatitis

    Breser, Maria L.; Lino, Andreia C.; Motrich, Ruben D.; Godoy, Gloria J.; Demengeot, Jocelyne; Rivero, Virginia E.
    This deposit is composed by a publication in which the IGC' authors have had the role of collaboration (it's a collaboration publication). This type of deposit in ARCA is in restrictedAccess (it can't be in open access to the public), and could only be accessed by two ways: either by requesting a legal copy to the author (the email contact present in this deposit) or by visiting the following link: https://www.nature.com/articles/srep33097

  19. Active regulatory T-cells contribute to broadened T-cell repertoire diversity in ivIg-treated SLE patients

    Costa, Nuno; Pires, Ana E; Gabriel, Ana M; Goulart, Luiz F; Pereira, Clara; Leal, Barbara; Queiros, Ana C; Chaara, Wahiba; Moraes-Fontes, Maria F; Vasconcelos, Carlos; Ferreira, Carlos; Martins, Jorge; Bastos, Marina; Santos, Maria J; Pereira, Maria A; Martins, Berta; Lima, Margarida; João, Cristina; Six, Adrien; Demengeot, Jocelyne; Fesel, Constantin
    Octapharma.

  20. Active regulatory T-cells contribute to broadened T-cell repertoire diversity in ivIg-treated SLE patients

    Costa, Nuno; Pires, Ana E; Gabriel, Ana M; Goulart, Luiz F; Pereira, Clara; Leal, Barbara; Queiros, Ana C; Chaara, Wahiba; Moraes-Fontes, Maria F; Vasconcelos, Carlos; Ferreira, Carlos; Martins, Jorge; Bastos, Marina; Santos, Maria J; Pereira, Maria A; Martins, Berta; Lima, Margarida; João, Cristina; Six, Adrien; Demengeot, Jocelyne; Fesel, Constantin
    Octapharma.

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