Faria, Ana Margarida; Filipe, Soraia; Lopes, Ana F.; Oliveira, Ana P.; Gonçalves, Emanuel João; Ribeiro, Laura
Número de acesso: WOS:000413690000014
Borges, Ana C.; Pereira, Nuno Marques; Franco, Maysa; Vale, Liliana; Pereira, Margarida; Cunha, Mónica V.; Amaro, Ana; Albuquerque, Teresa; Rebelo, Manuel
In the past two decades, zebrafish (Danio rerio)-based research has contributed to significant scientific advances. Still, husbandry and health programs did not evolve at the same pace, as evidenced by the absence of general guidelines. Health monitoring is essential to animal welfare, to permit animal exchanges across facilities, to contribute to robust experimental results, and for data reproducibility. In this study, we report a health program implemented in a zebrafish research facility to prevent, monitor, and control pathogen, and disease dissemination. This program includes quarantine, routine health screening of sentinels, and nonroutine screenings of retired animals and sick/moribund individuals. An...
Cerqueira, Marco; Millot, Sandie; Castanheira, Maria Filipa; Félix, A. S.; Oliveira, Gonçalo A.; Oliveira, C. C.; Martins, C. I.; Oliveira, Rui Filipe
The occurrence of emotions in non-human animals has been the focus of debate over the years.
Recently, an interest in expanding this debate to non-tetrapod vertebrates and to invertebrates has
emerged. Within vertebrates, the study of emotion in teleosts is particularly interesting since they
represent a divergent evolutionary radiation from that of tetrapods, and thus they provide an insight
into the evolution of the biological mechanisms of emotion. We report that Sea Bream exposed to
stimuli that vary according to valence (positive, negative) and salience (predictable, unpredictable)
exhibit different behavioural, physiological and neuromolecular states. Since according to the
dimensional theory of emotion valence and salience define a...
Bartels, Paul J.; Fontoura, Paulo; Nelson, Diane R.
Five marine arthrotardigrade species are recorded from Moorea, Society Islands, French Polynesia. Four were collected
from coral sand; two, Dipodarctus anaholiensis Pollock, 1995 and Florarctus kwoni Chang & Rho, 1997, are new records
for the region, and two, Halechiniscus perfectus Schulz, 1955 and Styraconyx kristenseni kristenseni Renaud-Mornant,
1981, have been previously reported. The fifth, a new species Styraconyx turbinarium sp. nov., is described and was collected
from the drifting brown alga Turbinaria ornata. The new species is characterized by the presence of peduncles on
all digits, an elongate primary clava, and the lateral cirrus A arising from a common pedestal and enveloped by a common
Neto, Carlos; Fonseca, João Paulo; Costa, José Carlos; Bioret, Frédéric
Omphalodes kuzinskyanae Willk. is an endangered annual plant of the family Boraginaceae, endemic to a
narrow coastal area in the Lisbon region (Portugal). Omphalodes littoralis Lehm. occurs in northwest Spain (subsp.
gallaecica) and northwest France (subsp. littoralis). Three approaches were used to assess the ecological requirements of
O. kuzinskyanae: (1) physical and chemical characterization of their habitat soil; (2) phytosociological analysis; (3)
comparison of several life history parameters under different light conditions. Germination experiments were conducted
to evaluate seed dormancy. The results show that O. kuzinskyanae occurs in thin sandy soil with a substantial amount of
organic matter and clay, mostly over limestone pavements. Phytosociological analysis...
Santos, Carla Sousa; Gil, Fátima; Almada, Vítor Carvalho
Pereira, Ricardo; Rismondo, Stefania; Caiano, Manuel; Pedroso, Silvia S; Fonseca, Paulo J.; Amorim, Maria Clara Pessoa
Animals often vocalize during territorial challenges as acoustic signals may
indicate motivation and fighting ability and contribute to reduce aggressive
escalation. Here, we tested the function of agonistic sounds in territorial
defence in the painted goby. Pomatoschistus pictus, a small vocal marine
fish that defends nests during the breeding season. We first measured the
number of times a male approached, avoided, explored, entered and
exited two unattended nests associated with either conspecific agonistic
sounds or a control: silence or white noise. Acoustic stimuli were played
back when the male approached a nest. In a second experimental set, we
added visual stimuli, consisting of a conspecific male in a small...
Augusto, Joana F.; Lopes, Patrícia Alexandra Rachinas; Santos, Manuel Eduardo dos
The resident population of common bottlenose dolphins (Tursiops truncatus) in the Sado Estuary, Portugal, has been declining
at least during the past three decades. A complete photographic census produced a current count of 24 animals—19 adults,
three subadults and two calves. It appears to be phylopatric and essentially closed, but given the likely importance that
exchanges with neighbouring coastal groups may play, even if rare, the most adequate term to define this dolphin should
be community and not population. Large groups with all age-classes are common in the community, possibly as a calf
and subadult protection strategy, and this may be related to the fact...
Almada, Vítor Carvalho; Almada, Frederico; Francisco, Sara Martins; Castilho, Rita; Robalo, Joana Isabel
The longspined bullhead (Taurulus bubalis, Euphrasen 1786) belongs to the family Cottidae and is a rocky shore species that
inhabits the intertidal zones of the Eastern Atlantic since Iceland, southward to Portugal and also the North Sea and Baltic,
northward to the Gulf of Finland, with some occurrences in the northern Mediterranean coasts eastward to the Gulf of
Genoa. We analysed the phylogeographic patterns of this species using mitochondrial and nuclear markers in populations
throughout most of its distributional range in west Europe. We found that T. bubalis has a relatively shallow genealogy with
some differentiation between Atlantic and North Sea. Genetic diversity was homogeneous...
Salgueiro, Emílio Eduardo Guerra; Nunes, Laura; Barros, Alexandra; Maroco, João; Salgueiro, Ana Isabel; Santos, Manuel Eduardo dos
Background: Interaction programs involving dolphins and patients with various pathologies or developmental
disorders (e.g., cerebral palsy, intellectual impairment, autism, atopic dermatitis, post-traumatic stress disorder,
depression) have stimulated interest in their beneficial effects and therapeutic potential. However, the true effects
observed in different clinical and psycho-educational setups are still controversial.
Results: An evaluation protocol consisting of the Childhood Autism Rating Scale (CARS), Psychoeducational
Profile-Revised (PEP-R), Autism Treatment Evaluation Checklist (ATEC), Theory of Mind Tasks (ToM Tasks) and a
custom-made Interaction Evaluation Grid (IEG) to evaluate behavioural complexity during in-pool interactions
was applied to 10 children diagnosed with Autism Spectrum Disorders. The ATEC, ToM Tasks and CARS results