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Repositório do Hospital Prof. Doutor Fernando Fonseca (3.582 recursos)

The Hospital Prof. Dr. Fernando Fonseca E.P.E. (HFF) institutional repository collects, preserves and disseminates the intellectual output of its professionals in digital format. Its aims are to increase the HFF’s investigation visibility and citation impact, to foster scientific communication and to preserve the HFF’s intellectual memory.

Patologia Clínica

Mostrando recursos 1 - 12 de 12

  1. Serotype 3 Remains the Leading Cause of Invasive Pneumococcal Disease in Adults in Portugal (2012-2014) Despite Continued Reductions in Other 13-Valent Conjugate Vaccine Serotypes

    Horácio, A; Silva-Costa, C; Lopes, J; Ramirez, M; Melo-Cristino, J; Portuguese Group for the Study of Streptococcal Infections
    Since 2010 the 13-valent pneumococcal conjugate vaccine (PCV13) replaced the 7-valent vaccine (PCV7) as the leading pneumococcal vaccine used in children through the private sector. Although, neither of the PCVs were used significantly in adults, changes in adult invasive pneumococcal disease (IPD) were expected due to herd protection. We characterized n = 1163 isolates recovered from IPD in adults in 2012-2014 with the goal of documenting possible changes in serotype prevalence and antimicrobial resistance. Among the 54 different serotypes detected, the most frequent, accounting for half of all IPD, were serotypes: 3 (14%), 8 (11%), 19A (7%), 22F (7%), 14...

  2. Characterization of B cells in healthy pregnant women from late pregnancy to post-partum: a prospective observational study

    Lima, J; Martins, C; Leandro, MJ; Nunes, G; Sousa, MJ; Branco, J; Borrego, LM
    BACKGROUND: B cells play a role in pregnancy due to their humoral and regulatory activities. To our knowledge, different maturational stages (from transitional to memory) of circulating B cell subsets have not yet been characterized (cell quantification and phenotype identification) in healthy pregnant women. Thus, the objective of our study was to characterize these subsets (as well as regulatory B cells) from late pregnancy to post-partum and to compare them with the circulating B cells of non-pregnant women. METHODS: In all of the enrolled women, flow cytometry was used to characterize the circulating B cell subsets according to the expression of IgD and...

  3. Sensibilidade das enterobacteriáceas produtoras de beta-lactamases de espectro alargado à fosfomicina

    Brito, T; Portugal, C; Sancho, L; Carvalho, C; Grima, B; Alves, J
    Introdução: A emergência de enterobacteriáceas produtoras de beta-lactamases de espectro alargado (ESBL) nos últimos anos representa um problema de saúde pública, escasseando alternativas eficazes para o seu tratamento. Vários trabalhos internacionais têm demonstrado uma sensibilidade in vitro muito elevada destas bactérias à fosfomicina, havendo alguns que testaram a eficácia clínica do tratamento de cistites agudas não complicadas no subgrupo das Escherichia coli com resultados promissores. No Hospital Prof. Dr. Fernando Fonseca EPE (HFF) tem havido um aumento anual progressivo do isolamento destes patogéneos. Os autores pretenderam testar a sensibilidade das enterobacteriáceas produtoras de ESBL à fosfomicina no HFF e averiguar o eventual potencial terapêutico. Material e métodos: Estudo prospectivo, durante 6 meses,...

  4. Determinants of the Sympatric Host-Pathogen Relationship in Tuberculosis

    David, S; Mateus, AR; Duarte, EL; Albuquerque, J; Portugal, C; Sancho, L; Lavinha, J; Gonçalves G, G
    Major contributions from pathogen genome analysis and host genetics have equated the possibility of Mycobacterium tuberculosis co-evolution with its human host leading to more stable sympatric host-pathogen relationships. However, the attribution to either sympatric or allopatric categories depends on the resolution or grain of genotypic characterization. We explored the influence on the sympatric host-pathogen relationship of clinical (HIV infection and multidrug-resistant tuberculosis [MDRTB]) and demographic (gender and age) factors in regards to the genotypic grain by using spacer oligonucleotide typing (spoligotyping) for classification of M. tuberculosis strains within the Euro-American lineage. We analyzed a total of 547 tuberculosis (TB) cases,...

  5. Prevalência dos utentes com tempo de tromboplastina parcial activado baixo, na população do Hospital Prof. Doutor Fernando da Fonseca, EPE

    Santos, MA; Aliyeva, E; Salazar, F; Silva, L; Sancho, L
    Introdução: A doença tromboembolica venosa (TEV) constitui um problema de grande impacto na saúde pública pela sua morbilidade e mortalidade. Tem uma etiologia multifactorial e os factores de risco são aditivos. Segundo vários estudos publicados a determinação de um tempo de tromboplastina parcial activado (TTPa) baixo, associa-se per se a um risco au- mentado de TEV. Objectivo: Cálculo da prevalência dos valores de TTPa inferiores ao Valor de Referência, VR, (excluíram-se utentes com trombofilias hereditárias/adquiridas). Materiais e Métodos: Realizou-se um estudo analítico observacional e longitudinal, no espaço temporal de Abril a Maio de 2014. Definiram-se três populações: 1ª - Dadores de...

  6. Duodeno-colic fistula as a rare presentation of lung cancer - surgical treatment of a stage IV oligometastatic lung disease

    Nunes, V; Santiago, I; Marinho, R; Pires, D; Theias, R; Gomes, A; Pignatelli, N
    INTRODUCTION: Rare adenosquamous carcinomas have no defined standard approach given their low incidence. They present with nonspecific imaging characteristics and are described as having worse prognosis than other lung malignancies, with greater likelihood of local invasion and early metastasis. PRESENTATION OF CASE: Male caucasian patient, 43 years, 26 pack-year smoking history, presented with watery diarrhea, early emesis and loss of 25% body weight (20kg) in four weeks. Colonoscopy identified a left colonic mass. Abdominal CT/ultrasound showed a large fistulous lesion between the 4th portion of the duodenum and left colon. CT showed a solid mass in the right upper lung lobe. Endoscopy and...

  7. From ANA-screening to antigen-specificity: an EASI-survey on the daily practice in European countries

    Damoiseaux, J; Agmon-Levin, N; Van Blerk, M; Chopyak, V; Eriksson, C; Heijnen, I; Herold, M; Høgåsen, K; Musset, L; Radice, A; Sousa, MJ, et al.
    OBJECTIVES: One of the main goals of the European Autoimmunity Standardisation Initiative (EASI) is the harmonisation of test-algorithms for autoantibodies related to systemic autoimmune rheumatic diseases (SARD). METHODS: A questionnaire was used to gather information on methodology, interpretation, and the algorithm for detection of anti-nuclear antibodies (ANA) in relation to their antigen-specificity. The questionnaire was sent to 1200 laboratories in 12 European countries. RESULTS: The response rate was 47.2%. The results reveal not only apparent differences between countries, but also within countries. CONCLUSIONS: Awareness of these differences may as such already stimulate harmonisation, but the observed differences may also direct recommendations that may further contribute to achieving...

  8. From ANA-screening to antigen-specificity: an EASI-survey on the daily practice in European countries.

    Damoiseaux, J; Agmon-Levin, N; Van Blerk, M; Sousa, MJ, et al.
    OBJECTIVES: One of the main goals of the European Autoimmunity Standardisation Initiative (EASI) is the harmonisation of test-algorithms for autoantibodies related to systemic autoimmune rheumatic diseases (SARD). METHODS: A questionnaire was used to gather information on methodology, interpretation, and the algorithm for detection of anti-nuclear antibodies (ANA) in relation to their antigen-specificity. The questionnaire was sent to 1200 laboratories in 12 European countries. RESULTS: The response rate was 47.2%. The results reveal not only apparent differences between countries, but also within countries. CONCLUSIONS: Awareness of these differences may as such already stimulate harmonisation, but the observed differences may also direct recommendations that may further contribute to...

  9. Counseling for non-invasive prenatal testing (NIPT): what pregnant women may want to know

    Oepkes, D; Yaron, Y; Kozlowski, P; Sousa, MJ, et al.

  10. Performance evaluation of a new fourth-generation HIV combination antigen-antibody assay

    Mühlbacher, A; Schennach, H; van Helden, J; Hebell, T; Pantaleo, G; Bürgisser, P; Sousa, JG, et al.
    Education and diagnostic tests capable of early detection represent our most effective means of preventing transmission of human immunodeficiency virus (HIV). The importance of early detection is underlined by studies demonstrating increased life expectancy following early initiation of antiviral treatment. The Elecsys(®) HIV combi PT assay is a fourth-generation antigen-antibody combination assay developed to allow earlier detection of seroconversion, and to have increased sensitivity and improved specificity. We aimed to determine how early the assay could detect infection compared with existing assays; whether all HIV variants could be detected; and the assay's specificity using samples from blood donors, routine specimens,...

  11. Staphylococcus aureus reservoirs and transmission routes in a Portuguese Neonatal Intensive Care Unit: a 30-month surveillance study.

    Conceição, T; Sousa, M; Miragaia, M; Paulino, E; Barroso, R; Brito, MJ; Sardinha, T; Sancho, L; Carreiro, H; Sousa, JG; Machado, MC; Lencastre, H
    Although Staphylococcus aureus is a major cause of outbreaks in neonatal intensive care units (NICUs), there are no studies on the epidemiology of S. aureus isolates responsible for infection in Portuguese NICUs. Between July 2005 and December 2007, a total of 54 methicillin susceptible S. aureus (MSSA) isolates were recovered from 16 infected infants, parents, health care workers (HCWs), and the environment in a level III NICU. Isolates were characterized by pulsed-field gel electrophoresis (PFGE), spa typing, and multilocus sequence typing. Virulence determinants were detected by multiplex polymerase chain reaction. Three major MSSA clones were endemic in the NICU, representing...

  12. Spreading of HIV-1 subtype G and envB/gagG recombinant strains among injecting drug users in Lisbon, Portugal.

    Esteves, A; Parreira, R; Piedade, J; Venenno, T; Franco, M; Sousa, JG; Patrício, L; Brum, P; Costa, A; Canas-Ferreira, W
    We have evaluated the genetic diversity of HIV-1 strains infecting injecting drug users (IDUs) in Lisbon, Portugal. Heteroduplex mobility assay and/or phylogenetic analysis revealed that env (C2V3C3 or gp41) subtype B is present in 63.7% of the 135 viral samples studied, followed by subtypes G (23.7%), A (6.7%), F (5.2%), and D (0.7%). Similar analysis of gag (p24/p7) performed on 91 of the specimens demonstrated that 49.5% of the infections were caused by subtype G viruses; other gag subtypes identified were B (39.5%), F (3.3%), A and D (1.1.% each), and the recombinant circulating form CRF02_AG (5.5%). Discordant env/gag sub-types...

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