Recursos de colección

Archivo Digital UPM (122.777 recursos)

This is an institutional repository providing access to the research output of the institution. Primarily contains thesis.

Materia = Biología

Mostrando recursos 1 - 20 de 84

  1. Open source technology in biomedical engineering: fast track towards sustainable development

    Ahluwalia, Arti; María, Carmelo de; Díaz Lantada, Andrés; Pietro, Licia Di; Ferretti, Jacopo; Magliaro, Chiara; Mridha, Mannan; Makobore, Philippa Ngaju; Madete, June; Aabloo, Alvo; Leibovits, Arni
    Recently the concept of Open Source Medical Devices (OSMD) has gained some attention, particularly with stunt-like displays of 3D printed prostheses capturing the imagination of the public. In this paper we argue that OSMD technology can be used to propel advancements in healthcare in developing countries in Africa, with equally beneficial consequences in more established economies, provided that the correct framework of training, sharing and safety and efficacy assessment is constructed.We demonstrate how such a framework can be created and show some examples of open-source engineering-based activities which we have conducted. The pros and cons of our approach are discussed...

  2. Design of a rib impactor equipment

    Torres, C. R.; García, G.; Aguilar, L. A.; Martínez Sáez, Luis
    The human ribs must be analyzed as long and as curved bones, due to their physiology. For the development of an experimental equipment that simulate the application of loads, over the rib in the moment of a frontal collision in an automobile with seat belt, it was applied a methodology that constituted in the identification of needs and the variables which led the design of 3D model, from this it was used the technique of fused deposition modeling for the development of the equipment pieces. The supports that hold the rib ends were design with two and three degrees of...

  3. UBORA: Euro-African Open Biomedical Engineering e-Platform

    Ravizza, Alice; Kitsing, Risto; Pahk, Mardo; Kallas, Kaspar; Díaz Lantada, Andrés; Pietro, Licia Di; María, Carmelo de; Ahluwalia, Arti
    1. Some reflections towards a new model 2. Collaborative biomedical engineering: Cases of success 3. The UBORA Project (Horizon 2020, EU-African collaboration) 4. The UBORA e-platform: Implementation and validation

  4. Characterization of type VI secretion systems (T6SS) of endosymbionts from mimosa or lupine

    Pacheco, Alba; Salinero, Álvaro; Bourebaba, Yasmina; Valle, Daniel del; Duran Wendt, David Ricardo; Boulila, Farida; Ormeño-Orrillo, E.; Martínez Romero, Esperanza; Imperial Ródenas, Juan; Ruiz Argüeso, Tomas-Andres; Palacios Alberti, Jose Manuel; Rey Navarro, Luis
    The T6SS is a nanosyringe that injects proteins into prokaryotic or eukaryotic cells, and it is encoded in the genomes of more than 25% of Gram-negative bacteria (1). We are studying the T6SS of Rhizobium etli Mim1 and Bradyrhizobium sp. LmicA16, symbionts of Phaseolus vulgaris/Leucaena leucocephala and Lupinus micranthus/Lupinus angustifolius/Spartium junceum, respectively. R. etli Mim1 contains a T6SS gene cluster organized in two divergent operons. When the T6SS is active, Hcp, a constituent of the secretory apparatus, can be detected in the extracellular medium (2). Hcp has been immunologically detected in the supernatant of Mim1 cultures. This protein was also...

  5. Breeding and scientific advances in the fight against Dutch elm disease - will they allow the use of elms in forest restoration?

    Martin Garcia, Juan Antonio; Sobrino-Plata, Juan; Rodríguez-Calcerrada, Jesús; Collada, Carmen; Gil Sanchez, Luis
    Revisión de los avances científicos y de producción de planta con fin de evaluar el potencial en la recuperación del olmo común.

  6. Bradyrhizobium sp endosymbiont bacteria nodulate several species of spontaneous leguminous plants of Genisteae tribe from northeastern of Algeria.

    Boulila, Farida; Bourebaba, Yasmina; Ahnia, Hadjira; Duran Wendt, David Ricardo; Imperial Ródenas, Juan; Ruiz Argüeso, Tomas-Andres; Rey Navarro, Luis
    218 bacterial isolates obtained from different legume species belonging to the Gensisteae tribe (Lupinus micruntus, Retama sphaerocarpa, Retama raetam and Cytisus vilosus) from different sampling sites in Algeria were studied. Cultivated on YMA medium these strains show a slow growth. The phylogenetic analysis based on 16S rDNA and the household genes of the representatives of these strains indicates that they belong to the genus Bradyrhizobium. A variety of Bradyrhizobium sp. (1, 2, 3) is present in this collection with the presence of strains that can lead to new species. (1) Boulila Farida et al., (2009). Syst. Appl. Microbiol. 32:245?255. (2)...

  7. Análisis de la adaptación de la fase endosimbiótica de Rhizobium leguminosarum bv. viciae a diferentes hospedadores

    Ballesteros Gutiérrez, Marta; Duran Wendt, David Ricardo; Domínguez Figueroa, J.; Alboreda Contreras, Marta; Palacios Alberti, Jose Manuel
    Los rizobios son alfa-proteobacterias capaces de infectar las raíces de las leguminosas e inducir en las mismas la formación de un nuevo órgano, el nódulo radicular. En dicho nódulo las células bacterianas, diferenciadas en bacteroides especializados en la fijación de nitrógeno, están rodeadas de una membrana peribacteroidal a través de cual la planta controla el intercambio de nutrientes hacia y desde el bacteroide. La adaptación de las bacterias al estilo de vida simbiótico es el resultado de un proceso de co-evolución entre ambos socios en el que se produce el intercambio de fuentes carbonadas y nitrógeno fijado en forma de...

  8. Caracterización del sistema de secreción de tipo VI en Rhizobium etli Mim1

    Salinero, Álvaro; Pacheco, Alba; Valle, Daniel del; Duran Wendt, David Ricardo; Ruiz Argüeso, Tomas-Andres; Imperial Ródenas, Juan; Martínez Romero, Esperanza; Ormeño-Orrillo, E.; Palacios Alberti, Jose Manuel; Rey Navarro, Luis
    La simbiosis rizobio-leguminosa es altamente específica. La translocación de proteínas denominadas efectores desde el citoplasma bacteriano a la célula vegetal es un elemento relacionado con dicha especificidad. Los efectores pueden ser translocados a través de diferentes sistemas de secreción. El análisis de genomas de rizobios ha permitido identificar en algunos la presencia de sistemas de secreción de tipo VI (T6SS). El T6SS tiene como componente principal una nanoestructura similar a las que utilizan los bacteriófagos1 para inyectar su ADN y que las bacterias usan para secretar proteínas. Los genes implicados en la formación de T6SS están agrupados y los que...

  9. Validation of the Hirst-Type Spore Trap for Simultaneous Monitoring of Prokaryotic and Eukaryotic Biodiversities in Urban Air Samples by Next-Generation Sequencing

    Nuñez Hernández, Andrés; Amo de Paz, Guillermo; Ferencova, Zuzana; Rastrojo, Alberto; Guantes, Raúl; García, Ana M.; Alcamí, Antonio; Gutiérrez-Bustillo, A. Montserrat; Moreno Gómez, Diego Alejandro
    Pollen, fungi, and bacteria are the main microscopic biological entities present in outdoor air, causing allergy symptoms and disease transmission and having a significant role in atmosphere dynamics. Despite their relevance, a method for monitoring simultaneously these biological particles in metropolitan environments has not yet been developed. Here, we assessed the use of the Hirst-type spore trap to characterize the global airborne biota by high-throughput DNA sequencing, selecting regions of the 16S rRNA gene and internal transcribed spacer for the taxonomic assignment. We showed that aerobiological communities are well represented by this approach. The operational taxonomic units (OTUs) of two...

  10. Mechanism of tolerance in Arabidopsis to cucumber mosaic virus infection

    Shukla, Aayushi
    Los virus son a menudo patógenos virulentos de las plantas, y éstas han desarrollado mecanismos defensivos que evitan o minimizan los efectos negativos de las infecciones virales. Las principales defensas de las plantas a los parásitos son la resistencia y la tolerancia. La resistencia es la capacidad del huésped de reducir la multiplicación del parásito, mientras que la tolerancia es la capacidad del huésped de reducir los efectos negativos de la infección en su eficacia biológica. Mientras la resistencia de las plantas a los virus ha recibido gran atención por parte de la investigación, poco se sabe de la tolerancia...

  11. Comparing Madrid and Salvador GHG emission inventories: implications for future researches

    Silveira Andrade, José Célio; Dameno Nielsen, Andrea; Pérez Rodríguez, Javier; Andrés Almeida, Juan Manuel de; Lumbreras Martin, Julio
    This paper compares the Greenhouse Gas (GHG) emission inventories of Madrid and Salvador and discusses some implications for future researches, focusing on citylevel carbon accounting (CLCA) of emissions from urban supply chains (USC) and final consumers. To carry out this study, secondary data were collected from official documents of municipal governments of these two cities. According to the results, there are differences in stationary energy GHG emissions due to the big distinction concerning electricity emission factors used by each city. Air transportation GHG emissions are also very different. These two cities share some common figures regarding road transportation and per...

  12. Genetic and biological characterization of Macrophomina phaseolina (Tassi) Goid. causing crown and root rot of strawberry

    Sánchez, Soledad; Chamorro, Manuel; Henríquez, José Luis; Grez, Javiera; Diaz Rodriguez, Isabel; Santos, Berta de los; Gambardella, Marina
    In recent years, crown and root rot of strawberry ( Fragaria × ananassa Duchesne ex Rozier) caused by Macrophomina phaseolina (Tassi) Goid. has affected strawberry production areas worldwide, and in developed countries its emergency has been attributed to the replacement of methyl bromide. The disease was reported in strawberry crop in Chile in 2013, in fields without fumigation. The use of resistant cultivars rises as an alternative to the management of this disease. The objective of this study was to perform a biological and molecular characterization of isolates obtained from two growing regions in Chile and Spain. A total of...

  13. Algunos rizobios van armados para luchar contra otras bacterias y para interaccionar con leguminosas

    Valle, Daniel del; Salinero, Álvaro; Pacheco, Alba; Rey Navarro, Luis
    Los rizobios son bacterias del suelo que pueden interaccionar con leguminosas específicas y multiplicarse en estructuras radiculares especializadas llamadas nódulos. En los nódulos las bacterias realizan la fijación biológica de nitrógeno proporcionando una ventaja a estas plantas para crecer en medios pobres en nitrógeno. La relación entre los rizobios y las leguminosas es específica y está mediada por diferentes señales químicas intercambiadas entre los actores. Una de esas señales son proteínas llamadas efectores, que secretan las bacterias al citoplasma vegetal. Se han descritos diferentes sistemas de secreción y uno de ellos se denomina sistema de secreción tipo VI (T6SS). Los...

  14. Establecimiento de indicadores armonizados para la evaluación del estado de conservación de los hábitats de bosque de la red Natura 2000.

    Espinosa Ruiz, S.; San Miguel Ayanz, Alfonso; Cañellas Rey de Viñas, I.; Alberdi Asensio, I.
    La Directiva Hábitats indica que los Estados miembros deben evaluar el estado de conservación de las especies y los tipos de Hábitats de Interés Comunitario sexenalmente, y conseguir que sea favorable. En España se desarrollaron indicadores para abordar la evaluación de 2012. Sin embargo, resulta necesario proceder a su armonización para unificar planteamientos, maximizar la eficiencia del proceso y optimizar el aprovechamiento de la información ya disponible. El objetivo principal de este estudio es proponer indicador es armonizados de estructura y funcionalidad para evaluar el estado de conservación de los hábitats de bosque de la red Natura 2000 en España,...

  15. Characterization of the ERECTA-YODA MAPK functional cascade and other phosphorylation events in innate immunity

    Swami, Sanjay
    Las cascadas de fosforilación son importantes mecanismos de transducción de señales que median entre la percepción de las señales y la activación de múltiples respuestas en las plantas. YODA (YDA) es una MAPK quinasa quinasa que funciona aguas abajo del receptor tipo quinasa (RLK) ERECTA (ER). Además de controlar el patrón estomático y otros procesos del desarrollo de la planta, la vía mediada por YDA-ER regula respuestas inmunitarias de Arabidopsis. La activación constitutiva de YDA en Arabidopsis (plantas CA-YDA) confiere resistencia a la enfermedad de amplio espectro. Un análisis transcriptómico mostró que las plantas CA-YDA sobreexpresan constitutivamente varios genes relacionados...

  16. An active Mitochondrial Complex II Present in Mature Seeds Contains an Embryo-Specific Iron-Sulfur Subunit Regulated by ABA and bZIP53 and Is Involved in Germination and Seedling Establishment

    Restovic, Franko; Espinoza Corral, Roberto; Gómez, Isabel; Vicente Carbajosa, Jesus; Jordana, Xavier
    Complex II (succinate dehydrogenase) is an essential mitochondrial enzyme involved in both the tricarboxylic acid cycle and the respiratory chain. In Arabidopsis thaliana, its iron–sulfur subunit (SDH2) is encoded by three genes, one of them (SDH2.3) being specifically expressed during seed maturation in the embryo. Here we show that seed SDH2.3 expression is regulated by abscisic acid (ABA) and we define the promoter region (114 to +49) possessing all the cis-elements necessary and sufficient for high expression in seeds. This region includes between 114 and 32 three ABRE (ABAresponsive) elements and one RY-enhancer like element, and we demonstrate that these...

  17. Shifts in microbial populations developed in Rusitec fermenters over the inclubation period differ between solid and liquid digesta phases and are influenced by diet

    Mateos, I.; Ranilla, M. J.; Saro, C.; Carro Travieso, Maria Dolores
    Rusitec fermenters are in vitro systems widely used to study ruminal fermentation, but little is known about the microbial populations establishing in them. This study was designed to assess the time evolution of microbial populations in fermenters fed medium- (MC; 50% alfalfa hay : concentrate) and high-concentrate diets (HC; 15 : 85 barley straw : concentrate). Samples from solid (SOL) and liquid (LIQ) content of fermenters were taken immediately before feeding on days 3, 8 and 14 of incubation for quantitative polymerase chain reaction and automated ribosomal intergenic spacer analysis analyses. In SOL, total bacterial DNA concentration and relative abundance...

  18. Analysis of fitness trade-offs limiting host range expansion in pepperinfecting tobamoviruses

    Bera, Sayanta
    La adquisición de nuevos huéspedes, es decir, la expansión de su gama de huéspedes, proporciona a un virus más oportunidades de transmitirse y sobrevivir, pero puede estar limitada por compromisos de eficacia entre los distintos huéspedes. Una causa principal de compromisos de eficacia entre huéspedes es la pleiotropía antagonista, es decir, que las mutaciones tengan efectos fenotípicos contrarios en los distintos huéspedes, una interacción Genotipo x Entorno. La epistasia, es decir una interacción Genotipo x Genotipo, puede ser también causa de compromisos de eficacia entre huéspedes. Como casos relevantes de la expansión de la gama de huéspedes podemos citar la...

  19. Sodium chloride accumulation in glycophyte plants with cyanobacterial symbionts.

    Green, Thomas George Allan; García Sancho, Leopoldo; Pintado, Ana; Saco, Dolores; Martín, Soleda; Arróniz Crespo, María; Casermeiro Martínez, Miguel Ángel; Cruz Caravaca, María Teresa de la; Cameron, Steven; Rozzi, Ricardo
    The majority of plant species are glycophytes and are not salt-tolerant and maintain low sodium levels within their tissues; if. high tissue sodium concentrations do occur, it is in response to elevated environmental salt levels. Here we report an apparently novel and taxonomically diverse grouping of plants that continuously maintain high tissue sodium contents and share the rare feature of possessing symbiotic cyanobacteria. Leaves of Gunnera magellanica in Tierra del Fuego always had sodium contents (dry weight basis) of around 4.26 g kg?1, about 20 times greater than measured in other higher plants in the community (0.29 g kg?1). Potassium...

  20. Molecular study of drought response in the mediterranean conifer Pinus pinaster Ait.: protein-coding-transcript profiling

    María, Nuria de; Perdiguero, Pedro; Guevara, Mª Ángeles; Cabezas, José Antonio; Chaves, Inés; Li, Zhen; Costa, Bruno; Vélez, Mª Dolores; Rodrigues, Andreia; Pizarro, Alberto; Díaz, Luis Manuel; Mancha, José Antonio; Collada, Carmen; Sánchez-Gómez, David; Aranda, Ismael; Sterck, Lieven; Díaz-Sala, Carmen; Miguel, Celia M.; Cervera, M. Teresa
    The aim ofthis work is to go a step further towards the understanding ofthe molecular mechanisms underlying maritime pine response to water stress and drought tolerance.

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