Palma, P.; Matos, C.; Alvarenga, P.; Kock-Schulmeyer, M.; Simões, I.; Barceló, D.; López de Alda, M.J.
The objective of the study was to assess the integrated use of macroinvertebrate indexes and ecotoxicological parameters
in the evaluation of the ecological status of a temporary stream with a strong agricultural influence.
Water quality was analysed at two sampling sites along the stream, considering: chemical supporting parameters;
hazardous substances (pesticides); benthic macroinvertebrate communities, through quality (Iberian Biological
Monitoring Working Party and Iberian Average Score Per Taxon) and multi-metric indices (Southern
Portuguese Index of Invertebrates and Ecological Quality Ratio); and ecotoxicological responses using lethal
and sub-lethal bioassays. The water chemical characterization showed high levels of organic matter and nutrients,
mainly in the dry period ((biochemical oxygen demand...
Monteiro, Filipa; Frese, Lothar; Castro, Sílvia; Duarte, Maria C.; Paulo, Octávio S.; Loureiro, João; Romeiras, Maria M.
Ramalho, José C.; Pais, Isabel P.; Leitão, António E.; Guerra, Mauro; Reboredo, Fernando H.; Máguas, Cristina M.; Carvalho, Maria L.; Scotti-Campos, Paula; Ribeiro-Barros, Ana I.; Lidon, Fernando J.C.; DaMatta, Fábio M.
Carvalho, Luísa C.; Coito, João L.; Gonçalves, Elsa F.; Lopes, Carlos; Amâncio, Sara
Extensive agricultural losses are attributed to heat, often combined with drought. These abiotic stresses occur in the field simultaneously, namely
in areas with Mediterranean climate, where grapevine traditionally grows. The available scenarios for climate change suggest an increase in the
frequency of heat waves and severe drought events in summer, also affecting the South of Portugal. In this work we monitored several productionrelated
parameters and evaluated the state of the oxidative stress response apparatus of two grapevine varieties, Touriga Nacional (TN) and
Trincadeira (TR), with and without irrigation, during three field seasons (2010 to 2012). Overall, results point to a high correlation of most...
Veloso, Maria Manuela; Simões-Costa, Maria Cristina; Carneiro, Luís C.; Guimarães, Joana B.; Mateus, Célia; Fevereiro, Pedro; Pinto-Ricardo, Cândido
The genetic diversity of “Gama” and “Bico de Corvo”, local cultivars of olive tree
(Olea europaea) from seven traditional orchards of Ficalho (Alentejo region, Portugal), was studied
to characterize the local diversity and assess the level of on farm diversity. Two different analytical
systems were used: endocarp morphological characteristics and genetic analysis by microsatellite
markers (Simple Sequence Repeats or SSR). The seven screened loci were polymorphic and allowed
the identification of 23 distinct SSR profiles within the 27 trees analyzed. A total of 52 different
alleles were scored, with an average of 7.43 alleles/SSR locus, and considerable genetic diversity
was found. Neighbor-Joining algorithm cluster analysis and principal...
Pinheiro, Ana Margarida; Carreira, Alexandra; Ferreira, Ricardo B.; Monteiro, Sara
Miao, Quingfeng; Shi, Haibin; Gonçalves, José M.; Pereira, L.S.
The sustainability of the Hetao Irrigation System, located in the water scarce upper Yellow
River basin, is a priority considering the need for water saving, increased water productivity, and
higher farmers’ incomes. The upgrading of basin irrigation, the main irrigation method, is essential
and includes the adoption of precise land levelling, cut-off management, improved water distribution
uniformity, and adequate irrigation scheduling. With this objective, the current study focuses on
upgrading wheat basin irrigation through improved design using a decision support system (DSS)
model, which considers land parcels characteristics, crop irrigation scheduling, soil infiltration,
hydraulic simulation, and environmental and economic impacts. Its use includes outlining water
saving scenarios and...
Menezes, Tiago; Romeiras, Maria M.; Sequeira, Miguel M.; Moura, Mónica
Karimi, Elham; Ramos, Miguel; Gonçalves, Jorge M.S.; Xavier, Joana R.; Reis, Margarida P. Reis; Costa, Rodrigo
Duvane, Jossias A.; Jorge, Tiago F.; Maquia, Ivete; Ribeiro, Natasha; Ribeiro-Barros, Ana I.F.; António, Carla
Bento, Miguel; Pereira, Sónia Gomes; Viegas, Wanda; Silva, Manuela
Lopes, Carlos M.; Torres, Albert; Guzman, Robert; Graça, João; Reyes, Miguel; Vitorino, Gonçalo; Braga, Ricardo; Monteiro, Ana; Barriguinha, André
This paper aims to present some results of the EU VINBOT (Autonomous cloud-computing vineyard robot to
optimize yield management and wine quality) project focused on vineyard yield estimation. A ground truth
evaluation trial was set up in an experimental vineyard with two plots of the white varieties ‘Alvarinho’ and
‘Arinto’, trained on a vertical shoot positioning system and spur pruned. For each varietal plot, six smart points
were selected with 10 contiguous vines each. During the ripening period of the 2016 season the vines were
manually assessed for canopy dimensions and yield and then scanned by the VINBOT sensor head composed
with a 2D laser rangefinder,...
Portugal, João; Monteiro, Ana; Luz, João Pedro
A implementação de culturas perenes, em Portugal, sofreu nas últimas décadas grandes alterações quer na instalação
da cultura quer nas técnicas de gestão. Este artigo revê a gestão das infestantes nas principais culturas perenes em
Portugal, o que se julga pertinente dado que se tem verificado a implementação de sistemas de modo de produção
biológica (MPB) e de produção integrada (MProdi). Por infestante, os autores, consideram as populações de uma dada
espécie vegetal que acima de determinados níveis e sob condicionalismos ecológicos particulares sejam responsáveis
por prejuízos “líquidos” (balanço benefícios-prejuízos negativo) inaceitáveis em termos económicos e/ou ecológicos ou
sociais. Primeiramente, apresentam-se os dados de 2015 relativamente...
Teixeira, Generosa; Monteiro, Ana
Most of the studies on Hypericum genus deals with the phytochemical and pharmacological
properties and the morphoanatomical data are scarce, although sometimes some species are poorly
identified. In order to obtain differentiating phytognostic characters, the micromorphological, anatomical
and histochemical study of mature leaves of six Hypericum species, H. androsaemum, H. calycinum, H.
canariense, H. grandifolium, H. x inodorum and H. patulum, was carried out using different techniques of
microscopy. Qualitative characters of both epidermal surfaces were evaluated: cell shape, cell wall,
epicuticular deposits and stomata type. Only the last three characters showed some kind of differences.
Five qualitative characters, stomata and gland indexes, total mesophyll thickness, palisade...
Tavares, Silvia; Amâncio, Sara
Sulfur is an essential nutrient to all plant species. Plants assimilate sulfur
in a well-described pathway, which has been taken up by roots. Regulatory mech-
anism has been the subject of many research papers. However, recent studies
highlighted differences between crop plants and the model plant Arabidopsis
thaliana. Our work focuses on the identification of genes involved in the sulfur
metabolism in the Vitis vinifera genome, and their response to sulfur deficiency and
other abiotic stress endured by grapevine in the field, namely water stress. Here, we
describe the identification and brief characterization of the first assimilation
enzymes involved in the sulfur pathway, the enzyme responsible for sulfur...
Ramos, Miguel Jesus Nunes; Coito, João Lucas; Fino, Joana; Cunha, Jorge; Silva, Helena; Almeida, Patrícia Gomes; Costa, Maria Manuela Ribeiro; Amâncio, Sara; Paulo, Octávio S.; Rocheta, Margarida
Key message RNA-seq of Vitis during early stages of
bud development, in male, female and hermaphrodite
flowers, identified new loci outside of annotated
gene models, suggesting their involvement in sex
Abstract The molecular mechanisms responsible for flower
sex specification remain unclear for most plant species. In the
case of V. vinifera ssp. vinifera, it is not fully understood what determines hermaphroditism in the domesticated subspecies
and male or female flowers in wild dioecious relatives (Vitis
vinifera ssp. sylvestris). Here, we describe a de novo assembly
of the transcriptome of three flower developmental stages
from the three Vitis vinifera flower types. The validation of
de novo assembly showed a correlation of 0.825....
Carvalho, Luísa C.; Silva, Marilia; Coito, João L.; Rocheta, Margarida P.; Amâncio, Sara
Cardoso, Andreia Saavedra; Domingos, Tiago; Magalhães, Manuela Raposo; Melo-Abreu, José Paulo; Palma, Jorge