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PANGAEA tech-keyword 'deNBIMolEcol' (25 data sets)

Mostrando recursos 1 - 20 de 759

  1. Gene expression via Fluidigm chip system on Western Baltic spring-spawning herring (Clupea harengus)

    Roth, Olivia

  2. Gene expression via Fluidigm chip system on Western Baltic spring-spawning herring (Clupea harengus)

    Roth, Olivia

  3. Experiment on effects of elevated pCO2 on bivalve dominated communities

    Schade, Hanna; Mevenkamp, Lisa; Guilini, Katja; Meyer, Stefanie; Gorb, Stanislav N; Abele, Doris; Vanreusel, Ann; Melzner, Frank
    Sandy communities were exposed to six different seawater pCO2 regimes for a total of three months (17.12.2011-06.03.2012) in a climate - controlled room. Six header tanks were continuously supplied with filtered seawater from Kiel Fjord, each one connected to six experimental units (EU) ensuring continuous seawater supply. Each EU consisted of a round plastic container with a volume of 12.5 L containing ca. 9.5 L of sediment and an overlying water column of ca. 3 L. The lower 10 cm of the sediment consisted of sieved sand taken from a local beach (Kiel, Falckenstein: 54°23,66 N; 10°11.56 E) while the...

  4. Experiment on effects of elevated pCO2 on bivalve dominated communities

    Schade, Hanna; Mevenkamp, Lisa; Guilini, Katja; Meyer, Stefanie; Gorb, Stanislav N; Abele, Doris; Vanreusel, Ann; Melzner, Frank
    Sandy communities were exposed to six different seawater pCO2 regimes for a total of three months (17.12.2011-06.03.2012) in a climate - controlled room. Six header tanks were continuously supplied with filtered seawater from Kiel Fjord, each one connected to six experimental units (EU) ensuring continuous seawater supply. Each EU consisted of a round plastic container with a volume of 12.5 L containing ca. 9.5 L of sediment and an overlying water column of ca. 3 L. The lower 10 cm of the sediment consisted of sieved sand taken from a local beach (Kiel, Falckenstein: 54°23,66 N; 10°11.56 E) while the...

  5. Individual codes, GenBank accession numbers and GenBank accession links for marine sponge-associated microbial metagenomes

    Horn, Hannes; Slaby, Beate M; Jahn, Martin T; Bayer, Kristina; Moitinho-Silva, Lucas; Förster, Frank; Abdelmohsen, Usama Ramadan; Hentschel, Ute
    Dataset contains metainformation to the samples used in the given pulication: links to Bioprojects, Biosamples, metagenome assemblies and raw data.

  6. Individual codes, GenBank accession numbers and GenBank accession links for marine sponge-associated microbial metagenomes

    Horn, Hannes; Slaby, Beate M; Jahn, Martin T; Bayer, Kristina; Moitinho-Silva, Lucas; Förster, Frank; Abdelmohsen, Usama Ramadan; Hentschel, Ute
    Dataset contains metainformation to the samples used in the given pulication: links to Bioprojects, Biosamples, metagenome assemblies and raw data.

  7. Individual codes, GenBank accession numbers and GenBank accession links for marine sponge-associated microbial metagenomes

    Horn, Hannes; Slaby, Beate M; Jahn, Martin T; Bayer, Kristina; Moitinho-Silva, Lucas; Förster, Frank; Abdelmohsen, Usama Ramadan; Hentschel, Ute
    Dataset contains metainformation to the samples used in the given pulication: links to Bioprojects, Biosamples, metagenome assemblies and raw data.

  8. Accession data for analysed Xestospongia testudinaria metatranscriptomes

    Jahn, Martin T; Markert, Sebastian M; Ryu, Taewoo; Ravasi, Timothy; Stigloher, Christian; Hentschel, Ute; Moitinho-Silva, Lucas
    Assigning functions to uncultivated environmental microorganisms continues to be a challenging endeavour. Here, we present a new microscopy protocol for fluorescence in situ hybridisation-correlative light and electron microscopy (FISH-CLEM) that enabled, to our knowledge for the first time, the identification of single cells within their complex microenvironment at electron microscopy resolution. Members of the candidate phylum Poribacteria, common and uncultivated symbionts of marine sponges, were used towards this goal. Cellular 3D reconstructions revealed bipolar, spherical granules of low electron density, which likely represent carbon reserves. Poribacterial activity profiles were retrieved from prokaryotic enriched sponge metatranscriptomes using simulation-based optimised mapping. We...

  9. Accession data for analysed Xestospongia testudinaria metatranscriptomes

    Jahn, Martin T; Markert, Sebastian M; Ryu, Taewoo; Ravasi, Timothy; Stigloher, Christian; Hentschel, Ute; Moitinho-Silva, Lucas
    Assigning functions to uncultivated environmental microorganisms continues to be a challenging endeavour. Here, we present a new microscopy protocol for fluorescence in situ hybridisation-correlative light and electron microscopy (FISH-CLEM) that enabled, to our knowledge for the first time, the identification of single cells within their complex microenvironment at electron microscopy resolution. Members of the candidate phylum Poribacteria, common and uncultivated symbionts of marine sponges, were used towards this goal. Cellular 3D reconstructions revealed bipolar, spherical granules of low electron density, which likely represent carbon reserves. Poribacterial activity profiles were retrieved from prokaryotic enriched sponge metatranscriptomes using simulation-based optimised mapping. We...

  10. Accession data for analysed Xestospongia testudinaria metatranscriptomes

    Jahn, Martin T; Markert, Sebastian M; Ryu, Taewoo; Ravasi, Timothy; Stigloher, Christian; Hentschel, Ute; Moitinho-Silva, Lucas
    Assigning functions to uncultivated environmental microorganisms continues to be a challenging endeavour. Here, we present a new microscopy protocol for fluorescence in situ hybridisation-correlative light and electron microscopy (FISH-CLEM) that enabled, to our knowledge for the first time, the identification of single cells within their complex microenvironment at electron microscopy resolution. Members of the candidate phylum Poribacteria, common and uncultivated symbionts of marine sponges, were used towards this goal. Cellular 3D reconstructions revealed bipolar, spherical granules of low electron density, which likely represent carbon reserves. Poribacterial activity profiles were retrieved from prokaryotic enriched sponge metatranscriptomes using simulation-based optimised mapping. We...

  11. Response of Arctic benthic bacterial deep-sea communities to different detritus composition during an ex-situ high pressure experiment

    Hoffmann, Katy; Hassenrück, Christiane; Salman-Carvalho, Verena; Holtappels, Moritz; Bienhold, Christina
    In a multidisciplinary ex situ experiment, benthic bacterial deep-sea communities from 2,500 m water depth at the Long-Term Ecological Research Observatory HAUSGARTEN (stationPS93/050-5 and 6), were retrieved using a TV-guided multiple corer. Surface sediments (0 - 2 cm) of 16 cores were mixed with sterile filtered deep-sea water to a final sediment dilution of 3.5 fold. The slurries were split and supplemented with five different types of habitat-related detritus: chitin, as the most abundant biopolymer in the oceans, and four different naturally occurring Arctic algae species, i.e. Thalassiosira weissflogii, Emiliania huxleyi, Bacillaria sp. and Melosira arctica. Incubations were performed in...

  12. Response of Arctic benthic bacterial deep-sea communities to different detritus composition during an ex-situ high pressure experiment

    Hoffmann, Katy; Hassenrück, Christiane; Salman-Carvalho, Verena; Holtappels, Moritz; Bienhold, Christina
    In a multidisciplinary ex situ experiment, benthic bacterial deep-sea communities from 2,500 m water depth at the Long-Term Ecological Research Observatory HAUSGARTEN (stationPS93/050-5 and 6), were retrieved using a TV-guided multiple corer. Surface sediments (0 - 2 cm) of 16 cores were mixed with sterile filtered deep-sea water to a final sediment dilution of 3.5 fold. The slurries were split and supplemented with five different types of habitat-related detritus: chitin, as the most abundant biopolymer in the oceans, and four different naturally occurring Arctic algae species, i.e. Thalassiosira weissflogii, Emiliania huxleyi, Bacillaria sp. and Melosira arctica. Incubations were performed in...

  13. Response of Arctic benthic bacterial deep-sea communities to different detritus composition during an ex-situ high pressure experiment

    Hoffmann, Katy; Hassenrück, Christiane; Salman-Carvalho, Verena; Holtappels, Moritz; Bienhold, Christina
    In a multidisciplinary ex situ experiment, benthic bacterial deep-sea communities from 2,500 m water depth at the Long-Term Ecological Research Observatory HAUSGARTEN (stationPS93/050-5 and 6), were retrieved using a TV-guided multiple corer. Surface sediments (0 - 2 cm) of 16 cores were mixed with sterile filtered deep-sea water to a final sediment dilution of 3.5 fold. The slurries were split and supplemented with five different types of habitat-related detritus: chitin, as the most abundant biopolymer in the oceans, and four different naturally occurring Arctic algae species, i.e. Thalassiosira weissflogii, Emiliania huxleyi, Bacillaria sp. and Melosira arctica. Incubations were performed in...

  14. Response of Arctic benthic bacterial deep-sea communities to different detritus composition during an ex-situ high pressure experiment

    Hoffmann, Katy; Hassenrück, Christiane; Salman-Carvalho, Verena; Holtappels, Moritz; Bienhold, Christina
    In a multidisciplinary ex situ experiment, benthic bacterial deep-sea communities from 2,500 m water depth at the Long-Term Ecological Research Observatory HAUSGARTEN (stationPS93/050-5 and 6), were retrieved using a TV-guided multiple corer. Surface sediments (0 - 2 cm) of 16 cores were mixed with sterile filtered deep-sea water to a final sediment dilution of 3.5 fold. The slurries were split and supplemented with five different types of habitat-related detritus: chitin, as the most abundant biopolymer in the oceans, and four different naturally occurring Arctic algae species, i.e. Thalassiosira weissflogii, Emiliania huxleyi, Bacillaria sp. and Melosira arctica. Incubations were performed in...

  15. Response of Arctic benthic bacterial deep-sea communities to different detritus composition during an ex-situ high pressure experiment

    Hoffmann, Katy; Hassenrück, Christiane; Salman-Carvalho, Verena; Holtappels, Moritz; Bienhold, Christina
    In a multidisciplinary ex situ experiment, benthic bacterial deep-sea communities from 2,500 m water depth at the Long-Term Ecological Research Observatory HAUSGARTEN (stationPS93/050-5 and 6), were retrieved using a TV-guided multiple corer. Surface sediments (0 - 2 cm) of 16 cores were mixed with sterile filtered deep-sea water to a final sediment dilution of 3.5 fold. The slurries were split and supplemented with five different types of habitat-related detritus: chitin, as the most abundant biopolymer in the oceans, and four different naturally occurring Arctic algae species, i.e. Thalassiosira weissflogii, Emiliania huxleyi, Bacillaria sp. and Melosira arctica. Incubations were performed in...

  16. Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors

    Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen
    The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression...

  17. Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors

    Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen
    The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression...

  18. Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors

    Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen
    The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression...

  19. Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors

    Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen
    The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression...

  20. Sampling information, length measurements, DNA alignments, models and phylogenetics trees for Oithona similis s.l.

    Cornils, Astrid; Wend-Heckmann, Britta; Held, Christoph
    Traditionally, many small-sized copepod species are considered to be widespread, bipolar or cosmopolitan. However, these large-scale distribution patterns need to be re-examined in view of increasing evidence of cryptic and pseudo-cryptic speciation in pelagic copepods. Here, we present a phylogeographic study of Oithona similis s.l. populations from the Arctic Ocean, the Southern Ocean and its northern boundaries, the North Atlantic and the Mediterrranean Sea. O. similis s.l. is considered as one of the most abundant species in temperate to polar oceans and acts as an important link in the trophic network between the microbial loop and higher trophic levels such...

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