ETD at Indian Institute of Science
(528 recursos)
Repository of Theses and Dissertations of Indian Institute of Science, Bangalore, India. The repository has been developed to capture, disseminate and preserve research theses of Indian Institute of Science.
Mostrando recursos 1 - 8 de 8
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MOLECULAR GENETIC ANALYSIS OF THE TUBEROUS SCLEROSIS COMPLEX - Mahmood Ali, Abdullah
Tuberous sclerosis complex (TSC) is an autosomal dominant disorder that affects several organs in the human body including the brain, heart, kidneys, eyes, skin, spleen, liver and lungs [Roach, et al., 1999]. TSC is characterized by hamartomas that rarely progress to malignancy in the affected organs. Clinical symptoms of TSC include cortical tubers and subependymal nodules in the brain, seizures, mental retardation, ungual and periungual fibromas, angiofibromas of the face, and angiomyolipomas in the kidneys [Roach, et al., 1999]. TSC displays genetic heterogeneity with two known loci: TSC1 on chromosome 9q34 [Fryer, et al., 1987a] and TSC2 on chromosome 16p13.3...
2.
Xenopus Laevis TGF-ß: Cloning And Characterization Of The Signaling Receptors - Mohan, D Saravana
The amphibian species Xenopus laevis, along with mouse and chicken is a very important model system, used widely to dissect the molecular intricacies of various aspects of vertebrate development. Study with Xenopus has clear advantages in terms of various technical considerations including the ease of handling early stage of embryos and due to the remarkable documentation of several early molecular events during development. The concept of inductive interactions between various cell types during early development was first revealed by the studies performed in Xenopus, and among the various factors proposed for mesoderm induction, the members of transforming growth factor-? (TGF-...
3.
Studies On The Phenomenon Of Blastocyst Hatching: Role Of Cysteine Proteases - Garimella, Sireesha V
The mammalian embryo is encased in a glycoproteinaceous covering, the
zona pellucida (ZP/zona) during preimplantation development. Prior to
implantation into the recipient maternal endometrium, the blastocyst has to
hatch out of this zona. This is a critical and an important event for the
successful establishment of pregnancy. Hatching in mammals is characterised
by the expansion of the blastocyst, followed by the nicking of the zona and
extrusion of the blastocyst by repeated contraction-expansion cycles, thereby
leaving the empty zona behind. In species such as the mouse, cow and
primates, the empty zona is left behind in the uterine lumen. However, in the
hamsters, the features associated...
4.
Molecular Analysis Of Hamster Sperm Capacitation: Significance Of Protein Tyrosine Phosphorylation - Naveen, Daniel M
Fertilization is a process that generates the first cell of a new organism. In mammals,
fertilization occurs in the female reproductive tract. The male gametes (spermatozoa) are rendered fertilization-competent only after they undergo capacitation and acrosome reaction (AR). The set of physiological changes, characterised by the acquisition of hyperactivated
motility, that render the spermatozoa fertilization competent is known as capacitation. Using in vitro models, the complex intracellular signaling events mediating this process are still
being understood. This thesis explores the role of protein tyrosine phosphorylation during capacitation using the golden hamster (Mesocricetus auratus) spermatozoa. The knowledge about the molecular components involved in...
5.
Gene Expression Profiling And Insights Into The Involvement Of The Insulin Signaling Pathway In Oral Cancer - Chakraborty, Sanjukta
1. Despite extensive research on oral squamous cell carcinoma (OSCC), its five-year survival rate has not improved for the last two decades. Effective treatment of OSCC requires the identification of molecular targets to design appropriate therapeutic strategies. To this end, DDRT-PCR analysis was used to identify molecular markers, which could be used as
therapeutic targets.
2. DDRT-PCR in combination with reverse Northern analysis identified 25 differentially expressed genes in oral tumors. Fourteen genes did not show homology to any known gene in the database and therefore may represent non-specific genomic DNA sequences or novel genes that have not yet...
6.
Receptor Guanylyl Cyclase C Cross-talk With Tyrosine Kinases And The Adaptor Protein, Crk - Vivek, T N
Signal transduction is a crucial event that enables cells to sense and respond to cues from their immediate environment. Guanylyl cyclase C (GC-C) is a member of the family of receptor guanylyl cyclases. GC-C is a single transmembrane protein that responds to its ligands by the production of the second messenger cGMP. The guanylin family of peptides, (including the bacterially produced heat-stable enterotoxin ST) is the ligand for GC-C, elevates intracellular cGMP levels and activates downstream pathways. GC-C regulates the cystic fibrosis transmembrane conductance regulator (CFTR) by inducing phosphorylation by protein kinase G, resulting in chloride ion and fluid efflux....
7.
Expression Profiling Of Genes Regulated By TGF-β : Role Of Multiple Signaling Pathways - Ranganathan, Prathibha
Transforming growth factor-β (TGF-β) is the proto-type member of a super family of secreted proteins comprised of several structurally related, but functionally divergent proteins like the BMP, activin, inhibin, mullerian inhibitory substance etc. TGF-β was originally identified as a secreted factor, which in the presence of EGF was capable of transforming normal rat kidney fibroblasts. Studies over the years have shown that this protein is multifunctional that influences several processes including development, immune function, epithelial cell growth and motility, wound healing etc. TGF-β plays important role in the normal physiology as well as in pathological conditions in mammals. There are...
8.
Evolution Of New Metabolic Functions By Mutations In Pre-Existing Genes : The chb Operon Of Escherichia Coli As A Paradigm - Kachroo, Aashiq Hussain
Escherichia coli has the ability to respond to stress such as starvation in a very efficient manner. Under conditions of starvation wherein a novel substrate is provided as a sole nutritional source,
Spontaneous mutants arise in a population of E.Coli that are able to utilize this novel carbon Many generic systems, upon mutational activation, have been shown to allow E.coli to Grow on novel substrates. .
Wildtype E.coli is not able to utilize cellobiose, a disaccharide of glucose, as a carbon source. However after prolonged incubation with cellobiose as a sole carbon source, spontaneous Cel+ mutants can be isolated. The...
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