Cornamusaz, Rita; Morais, Zilda
Poster presented at the 2nd International Congress of CiiEM: “Translational Research and Innovation in Human in health Sciences”. Egas Moniz, Caparica, 11-13 June 2017
Canadas, Raphaël F.; Cavalheiro, João M.B.T.; Guerreiro, João D.T.; Almeida, M. Catarina M.D. de; Pollet, Eric; Silva, Cláudia Lobato da; Fonseca, M.M.R. da; Ferreira, Frederico Castelo
"This integrated study shows that waste glycerol can be bio-valorized by the fabrication of electrospun scaffolds for stem cells. Human mesenchymal stem cells (hMSC) provide an interesting model of regenerating cells because of their ability to differentiate into osteo-, chrondro-, adipo- and myogenic lineages. Moreover, hMSC have modulatory properties with potential on treatment of immunologic diseases. Electrospun fiber meshes offer tunable mechanical and physical properties that can mimic the structure of the native extracellular matrix, the natural environment where cells inhabit. Following a biorefinery approach, crude glycerol directly recovered from a biodiesel post-reaction stream was fed as major C source...
Carvalho, Inês; Tomé, Sara; Neto, Miguel; Dinis, Isabel
"Os abcessos epidurais são infeções muito graves do Sistema Nervoso Central. Constituem lesões focais confinadas ao crânio ou coluna vertebral que exercem compressão cerebra lou medular causando sintomas severos e complicações permanentes. Este caso clínico constitui a descrição de uma complicação pós-operatória após uma artroplastia total do joelho.Embora seja uma entidade rara, tem-se vindo a verificar um aumento de incidência nos últimos 30 anos que se deve, não só aos avanços tecnológicos no campo da imagiologia,mas também ao envelhecimento progressivo da população e ao acréscimo do número de anestesias regionais. A chave para o tratamento desta complicação é o seu...
Carvalho, Inês; Costa, Gabriela; Lages, Neusa; Correia, Carlos
"Introdução: O bloqueio do plexo lombar é uma técnica loco-regional, útil para anestesia/analgesia seletiva de cirurgias da região anca e faces anterolateral e medial da coxa. Este trabalho tem como objetivos verificar da eficácia analgésica fundamentalmente da fase pós-operatória, bem como a incidência de efeitos secundários e complicações em comparação com outras técnicas analgésicas.
Material e Métodos: Estudo retrospetivo sobre a casuística desta técnica, eficácia e complicações. Procedeu-se à colheita de dados da Unidade de Dor Aguda da totalidade de 93 doentes submetidos a bloqueio do plexo lombar por técnica dual guidance, neuroestimulação e ecografia por abordagem posterior durante os anos...
Querido, Sara; Carvalho, Inês; Moleiro, Filipa; Póvoa, Pedro
"An otherwise healthy 37-year-old man was admitted to hospital with uncontrollable vomiting and abdominal pain. Lithiasic acute pancreatitis was diagnosed on the basis of clinical symptoms along with raised serum amylase levels and compatible findings in ultrasonography and CT scan. Two Ranson criteria (lactate dehydrogenase over 350 U/L and aspartate aminotransferase over 250 U/L) were present at admission. The patient was transferred to an intensive care unit (ICU); intravenous crystalloids were prescribed and analgaesics were administered for pain relief. Unexpectedly, 10 h after ICU admission, he presented a cardiac arrest with a non-defibrillate rhythm and died after 40 min of advanced life support. An...
Romão, Daniela; Sabino, Raquel; Veríssimo, Cristina; Viegas, Carla; Barroso, Helena; Duarte, Aida; Solo-Gabriele, Helena; Gunde-Cimerman, Nina; Babič, Monika Novak; Marom, Tal; Brandão, João
Sand serves as a reservoir for potentially pathogenic microorganisms. Children, a high-risk group, can acquire infections from sand in sandboxes, recreational areas, and beaches. This paper reviews the microbes in sands, with an emphasis on fungi. Recreational areas and beach sands have been found to harbor many types of fungi and microbes. A newly emerging group of fungi of concern include the black yeast-like fungi. After establishing that sand is a reservoir for fungi, clinical manifestations of fungal infections are described with an emphasis on ocular and ear infections. Overall, we recommend environmental studies to develop monitoring strategies for sand...
Proença, L.; Barroso, H.; Figueiredo, N.; Lino, A. R.; Capelo, S.; Fonseca, I. T. E.
"The corrosion resistance of Wiron®88, a Ni–Cr–Mo alloy, was evaluated in liquid growth media in the absence and presence of the Streptococcussobrinus and Streptococcus mutans strains. Open circuit potential measurements, cyclic voltammetry, linear sweep voltammetry, as well as electronic microscopy coupled to electron diffraction spectroscopy (SEM/EDS), were the main techniques used in this study. It was concluded that the presence of S. sobrinus and S. mutans have only a slight effect on the corrosion resistance of the Wiron®88 alloy, with the S. mutans being slightly more aggressive. For both strains the corrosion resistance Rp is of the same order (kΩ...
Solo-Gabriele, Helena M.; Harwood, Valerie J.; Kay, David; Fujioka, Roger S.; Sadowsky, Michael J.; Whitman, Richard L.; Wither, Andrew; Caniça, Manuela; Fonseca, Rita Carvalho da; Duarte, Aida; Edge, Thomas A.; Gargaté, Maria J.; Gunde-Cimerman, Nina; Hagen, Ferry; McLellan, Sandra L.; Silva, Alexandra Nogueira da; Babič, Monika Novak; Prada, Susana; Rodrigues, Raquel; Romão, Daniela; Sabino, Raquel; Samson, Robert A.; Segal, Esther; Staley, Christopher; Taylor, Huw D.; Veríssimo, Cristina; Viegas, Carla; Barroso, Helena; Brandão, João C.
"Recent studies suggest that sand can serve as a vehicle for exposure of humans to pathogens at beach sites, resulting in increased health risks. Sampling for microorganisms in sand should therefore be considered for inclusion in regulatory programmes aimed at protecting recreational beach users from infectious disease. Here, we review the literature on pathogen levels in beach sand, and their potential for affecting human health. In an effort to provide specific recommendations for sand sampling programmes, we outline published guidelines for beach monitoring programmes, which are currently focused exclusively on measuring microbial levels in water. We also provide background on...
Resende, A.; Amorim, A.; Silva, C. Vieira da; Ribeiro, T.; Porto, M. J.; Santos, J. Costa; Costa, H. Afonso
"Cabo Verde is located on the West African coast, was discovered uninhabited by Portuguese explorers, colonized by European and African slaves and is culturally characterized by a mixture of their settlers. Cabo Verde is one of the countries with the highest number of emigrants living in Portugal.
In order to validate the genetic markers included in the Combined DNA Index System and in the European Standard Set, samples from 500 unrelated individuals from Cabo Verde, currently living in Lisboa, were studied with the PowerPlex® Fusion System. To implement the genotyping method at the Serviço de Genética e Biologia Forenses (SGBF) we...
Morais, Paulo; Amorim, António; Silva, Cláudia Vieira da; Ribeiro, Teresa; Santos, Jorge Costa; Costa, Heloísa Afonso
"The aims of this study were (i) to enrich mtDNA global database, (ii) obtainment of the mtDNA variability of the Cabo Verde population living in Lisboa to complement previous studies by our group using STR genetic markers (Amorim et al. 2012; Afonso Costa et al. 2014), (iii) assign haplotypes to designated haplogroups, (iv) infer whether there are genetic proximity between the studied population and previous studies according to the mtDNA profile of the Cabo Verde population, and (v) compare the studied population with other African populations, with the aim to bring more light to our understanding on the subject of...
Dario, Paulo; Mouriño, Helena; Oliveira, Ana Rita; Lucas, Isabel; Ribeiro, Teresa; Porto, Maria João; Santos, Jorge Costa; Dias, Deodália; Real, Francisco Corte
"DNA phenotyping research is one of the most emergent areas of forensic genetics. Predictions of externally visible characteristics are possible through analysis of single nucleotide polymorphisms. These tools can provide police with “intelligence” in cases where there are no obvious suspects and unknown biological samples found at the crime scene do not result in any criminal DNA database hits. IrisPlex, an eye color prediction assay, revealed high prediction rates for blue and brown eye color in European populations. However, this is less predictive in some non-European populations, probably due to admixing. When compared to other European countries, Portugal has a...
Guerreiro, João da Silva; Casoni, Dianne; Santos, Jorge Costa
"The goal of this research is to provide a general portrait of forensic psychological reports under the Portuguese justice system. An analysis of 106 reports in terms of their relevance and coherence was conducted since these two dimensions appear to be key to better understanding some of the specific characteristics related to the overall quality of forensic psychological reports. A grid was constructed to identify elements related to these two dimensions and its application show that, while mostly meeting formal characteristics such as adequate organization, most of the reports surveyed fell short of meeting the criteria defining relevance and coherence....
Simão, Filipa; Costa, Heloísa Afonso; Silva, Cláudia Vieira da; Ribeiro, Teresa; Porto, Maria João; Santos, Jorge Costa; Amorim, António
"Portugal has been considered a country of emigrants, nevertheless in the past decades the number of immigrants has grown throughout all the country. This migratory flux has contributed to a raise of heterogeneity at multiple levels. According to statistical data, at the end of 2012 the total number of Angolan immigrants in Portugal equalled about 20,000 individuals. A territorial predominance has been found for the metropolitan region of Lisboa. Angola is a country located in the Atlantic coast of Africa. The presence of Bantu people and the colonisation by Portuguese people on Angolan territory are considered to be the major...
Santos, Carla Adriana; Pereira, Marta; Martins, Vera Santos; Fonseca, Jorge
tracheoesophageal fistula (TEF) may result from cancer or mechanical ventilation. Endoscopic Gastrostomy or Gastrojejunostomy (PEG/PEG-J) is used for nutritional support.
in TEF-patients, evaluating nutritional status when PEG is performed, safety of PEG/PEG-J and clinical outcome.
from the files of PEG/PEG-J feed TEF-patients we collected: clinical data, Body Mass Index, albumin, transferrin and cholesterol when gastrostomy was performed, and clinical outcome globally and according with the TEF cause: Group 1: complication of mechanical ventilation, Group 2: cancer.
twelve patients, 18-91 years (median: 53), 11 PEG, one PEG-J: six complications of ventilation (neurological diseases), 6 cancers. Mean period from TEF diagnosis until gastrostomy: 2 months...
Santos, Carla Adriana; Fonseca, Jorge; Carolino, Elisabete; Lopes, Teresa; Guerreiro, António Sousa
"BACKGROUND AND AIMS:
endoscopic gastrostomy (PEG) patients usually present protein-energy malnutrition, but little is known about selenium deficiency. We aimed to assess serum selenium evolution when patients underwent PEG, after 4 and 12 weeks. We also evaluated the evolution of albumin, transferrin and Body Mass Index and the influence of the nature of the underlying disease.
a blood sample was obtained before PEG (T0), after 4 (T1) and 12 (T3) weeks. Selenium was assayed using GFAAS (Furnace Atomic Absorption Spectroscopy). The PEG patients were fed through homemade meals. Patients were studied as a whole and divided into two groups: head and neck...
Fonseca, Jorge; Adriana, Carla; Fróis-Borges, Miguel; Meira, Tânia; Oliveira, Gabriel; Santos, José Carlos
Head and neck cancer (HNC) patients tend to develop dysphagia. In order to preserve the nutritional support, many undergo endoscopic gastrostomy (PEG). In HNC patients, ostomy metastasis is considered a rare complication of PEG, but there are no reports of successful treatment of these metastatic cancers. We report the case of a 65 years old pharyngeal/laryngeal cancer patient who underwent a PEG before the neck surgery. He was considered to be cured, resumed oral intake and the PEG tube was removed. Ten months after, he returned with a metastasis at the ostomy site. A block resection of the stomach and...
Santos, Carla Adriana; Fonseca, Jorge; Brito, José; Fernandes, Tânia; Gonçalves, Luísa; Guerreiro, António Sousa
"Background and aims: Dysphagic patients who underwent endoscopic gastrostomy (PEG) usually present protein-energy malnutrition, but little is known about micronutrient malnutrition. The aim of the present study was the evaluation of serum zinc in patients who underwent endoscopic gastrostomy and its relationship with serum proteins, whole blood zinc, and the nature of underlying disorder. Methods: From patients that underwent gastrostomy a blood sample was obtained minutes before the procedure. Serum and whole blood zinc was evaluated using Wavelength Dispersive X-ray Fluorescence Spectroscopy. Serum albumin and transferrin were evaluated. Patients were studied as a whole and divided into two groups: head...
Correira Pereira, Marta Alexandra; Santos, Carla Adriana; Brito, José Almeida; Fonseca, Jorge
Introduction: Gastrostomy fed head or neck cancer patients frequently have impaired speech capacities. Enteral feeding teams frequently depend on laboratorial or anthropometrical parameters for nutritional assessment.
Aims: In these patients, this study aimed to evaluate: (1) the practicability of Scored - Patient-Generated Subjective Global Assessment (PG-SGA); (2) their nutritional status using the Scored-PG-SGA; (3) association of serum albumin and transferrin values to the nutritional status rating using PG-SGA.
Methods: On adult outpatients with head or neck cancer under prolonged (> 1 month) gastrostomy feeding, Scored-PG-SGA, albumin and transferrin were evaluated during the same appointment.
Results: Scored-PG-SGA was easily feasible in...
Fonseca, J.; Meira, T.; Nunes, A.