Waap, Silke; Symondson, William O. C.; Granadeiro, José Pedro; Alonso, Hany Rafael de Drummond Ludovice Garcia; Gonçalves, Catarina Serra; Dias, Maria P.; Catry, Paulo
Existe informação suplementar disponível na versão online.
Castilho, Rita; Cunha, Regina L.; Faria, Cláudia Barreiros Macedo de; Velasco, Eva Maria; Robalo, Joana Isabel
Transition zones are of high evolutionary interest because unique patterns of spatial variation are often retained. Here, we investigated the phylogeography of the peacock blenny, Salaria pavo, a small marine intertidal fish that inhabits rocky habitats of the Mediterranean and the adjacent Atlantic Ocean. We screened 170 individuals using mitochondrial and nuclear sequence data from eight locations. Four models of genetic structure were tested: panmixia, isolation-by-distance, secondary contact and phylogeographic break. Results indicated clear asymmetric migration from the Mediterranean to the Atlantic but only marginally supported the isolation-by-distance model. Additionally, the species displays an imprint of demographic expansion compatible with...
Almada, Frederico; Carpi, Piera; Carlos, Fábio Ferreira; Francisco, Sara Martins; Faria, Ana Margarida; Lungu, Ruxanda; Coelho, Pedro Duarte; Neto, José; Gonçalves, Emanuel João; Robalo, Joana Isabel
A simple PCR-RFLP
consisting in the digestion of a fragment of the 12S ribosomal
RNA (12SrRNA) with species-specific
restriction enzymes (MboII and HinfI) was developed
to distinguish northeastern Atlantic species in the genus Atherina. Unambiguous
identification of morphologically similar sympatric species is frequently difficult, especially
when younger specimens are included in the analysis. Genetic and morphological
analysis of a total of 123 atherinids collected near the mouth of the Tagus estuary
(west Portugal) confirmed that only Atherina presbyter (sand smelt) adults and juveniles
are recurrently using these marine rocky habitats as spawning and nursery grounds,
respectively. The importance of applying non-destructive
methods is emphasized. In
the future, the conditions involved in the...
Lopes, Patrícia Rachinas; Luís, Ana Rita; Borges, Ana Sofia; Neto, Márcia P.; Santos, Manuel Eduardo dos
Common bottlenose dolphins (Tursiops trunca-tus) produce a range of underwater vocalizations, both pulsed (echolocation clicks and burst-pulses) and nonpulsed (whistles). Whistles may be emit-ted in stereotyped (signature) or variant patterns, and their production might be affected by sex, age, environmental, and social contexts. This study examined, non-intrusively, the whistle emissions of six captive bottlenose dolphins at Zoomarine in Algarve, Portugal, in two separate time sets and three different contexts: two of the animals in isolation in 2008, and all six in 2012, both seg-regated from their group and in social context. From a total of 1,681 whistles, 1,249 were ana-lyzed...
Dias, Ester; Morais, Pedro; Faria, Ana Margarida; Antunes, Carlos; Hoffman, Joel C.
Identifying nursery habitats is of paramount importance
to define proper management and conservation
strategies for flatfish species. Flatfish nursery
studies usually report upon habitat occupation, but
few attempted to quantify the importance of those
habitats to larvae development. The reliance of two
sympatric flatfish species larvae, the European flounder
Platichthys flesus and the common sole Solea
solea, on the estuarine food web (benthic versus
pelagic), was determined through carbon and nitrogen
stable isotope analysis. The organic matter
sources supporting the production of P. flesus and
S. solea larvae biomass originates chiefly in the benthic
food web. However, these species have significantly
different d13C and d15N values which suggest
that they prey on organisms that use...
Almada, Frederico; Francisco, Sara Martins; Lima, Cristina Silva; Fitzgerald, Richard; Mirimin, Luca; Villegas-Ríos, David; Saborido-Rey, Fran; Afonso, Pedro; Morato, Telmo; Bexiga, Sérgio; Robalo, Joana Isabel
Material suplementar, disponível online em: https://dx.doi.org/10.6084/m9.figshare.c.3683176.
Costa, Barbara Horta e; Claudet, Joachim; Franco, Gustavo; Erzini, Karim; Caro, Anthony; Gonçalves, Emanuel João
Dudley et al.  commented on our paper , arguing that the current IUCN objective-based categorization of
protected areas, which is also used in marine protected areas (MPAs), should not be abandoned and replaced by
the new regulation-based classification system . Here we clarify that we do not advocate replacing the
current IUCN categories, but highlight the benefits of using both the objective-based IUCN categories and the
new regulation-based classification when applied to MPAs. With an increasing number of MPA types being
implemented, most of them multiple-use areas zoned for various purposes, assessing ecological and socioeconomic
benefits is key for advancing conservation targets and policy objectives....
Solomon, F. N.; Rodrigues, Diana; Gonçalves, Emanuel João; Serrão, Ester A.; Borges, Rita Alexandra
Larval development and allometric growth patterns of the black-faced blenny Tripterygion delaisi are described from a larval series (body length, LB = 3·30-12·10 mm) caught by light traps at the Arrábida Marine Park, Portugal. Larvae of T. delaisi possess distinctive morphometric and meristic characteristics which can be used to identify this species from related taxa. Pigmentation is sparse but characteristic, consisting of pigmented eyes, gas bladder pigmentation in the dorsal region, anal pigmentation and a row of regularly spaced postanal ventral melanophores. This pattern is present from as early as the yolk-sac stage and persists throughout all stages with just the addition...
Santos, Erika; Rocha, Clélia M. C. da; Gomes, Edivaldo Júnior; Fontoura, Paulo
Three new tardigrade species, Batillipes brasiliensis sp. nov., Batillipes dandarae sp. nov. and Batillipes potiguarensis
sp. nov., are described from shallow subtidal sediments of the Brazilian coast. B. brasiliensis sp. nov. and B. dandarae
sp. nov. have toes 3 and 4 on leg IV different in length, so they can be included in the D group of species, while B. potiguarensis
sp. nov., with toes 3 and 4 on leg IV equal in length belong to the A group. Batillipes brasiliensis sp. nov. is
characterized by having an ala-like caudal expansion; cuticular projections on the coxal region of legs I-III, and lateral
projections. The lateral...
Castro, Joana M.; Amorim, Maria Clara Pessoa; Oliveira, Ana P.; Gonçalves, Emanuel João; Munday, Philip L.; Simpson, Stephen D.; Faria, Ana Margarida
Atmospheric CO2 levels have been increasing at an unprecedented rate due to anthropogenic activity. Consequently, ocean pCO2 is increasing and pH decreasing, affecting marine life, including fish. For many coastal marine fishes, selection of the adult habitat occurs at the end of the pelagic larval phase. Fish larvae use a range of sensory cues, including sound, for locating settlement habitat. This study tested the effect of elevated CO2 on the ability of settlement-stage temperate fish to use auditory cues from adult coastal reef habitats. Wild late larval stages of painted goby (Pomatoschistus pictus) were exposed to control pCO2 (532 μatm,...
Patrício, Ana R.; Diez, Carlos E.; Dam, Robert P. van; Godley, Brendan J.
Outbreaks of fibropapillomatosis (FP), a neoplastic infectious disease of marine turtles,
have occurred worldwide since the 1980s. Its most likely aetiological agent is a virus, but disease
expression depends on external factors, typically associated with altered environments. The
scarcity of robust long-term data on disease prevalence has limited interpretations on the impacts
of FP on turtle populations. Here we model the dynamics of FP at 2 green turtle foraging aggregations
in Puerto Rico, through 18 yr of capture-mark-recapture data (1997−2014). We observed
spatiotemporal variation in FP prevalence, potentially modulated via individual site-fidelity. FP
ex pression was residency dependent, and FP-free individuals developed tumours after 1.8 ± 0.8...
Klein, Maria; Teixeira, Sara; Assis, Jorge; Serrão, Ester A.; Gonçalves, Emanuel João; Borges, Rita Alexandra
Adults of most marine benthic and demersal fish are site-attached, with the dispersal of their larval stages ensuring connectivity among populations. In this study we aimed to infer spatial and temporal variation in population connectivity and dispersal of a marine fish species, using genetic tools and comparing these with oceanographic transport. We focused on an intertidal rocky reef fish species, the shore clingfish Lepadogaster lepadogaster, along the southwest Iberian Peninsula, in 2011 and 2012. We predicted high levels of self-recruitment and distinct populations, due to short pelagic larval duration and because all its developmental stages have previously been found near...
Furtado, Ricardo Miranda; Menezes, Dilia; Santos, Carolina Jardim; Catry, Paulo
Marine plastic pollution is rapidly growing and is a source of major concern. Seabirds often ingest plastic debris and are increasingly used as biological monitors of plastic pollution. However, virtually no studies have assessed plastics in seabirds in the deep subtropical North Atlantic. We investigated whether remains of white-faced storm-petrels (WFSP) present in gull pellets could be used for biomonitoring. We analysed 263 pellets and 79.0% of these contained plastic debris originating in the digestive tract of WFSP. Pellets with no bird prey did not contain plastics. Most debris were fragments (83.6%) with fewer plastic pellets (8.2%). Light-coloured plastics predominated...
Chivers, Douglas P.; McCormick, Mark I.; Allan, Bridie J. M.; Mitchell, Matthew D.; Gonçalves, Emanuel João; Bryshun, Reid; Ferrari, Maud C. O.
Many vertebrates are known to show behavioural lateralization, whereby they differentially use one side of their body or either of their bilateral organs or limbs. Behavioural lateralization often manifests in a turning bias in fishes, with some individuals showing a left bias and others a right bias. Such biases could be the source of considerable conflict in fish schools given that there may be considerable social pressure to conform to the group to maintain effective group evasion. Here, we show that predation pressure is a major determinant of the degree of lateralization, both in a relative and absolute sense, in...
Félix, Pedro M.; Amorim, Maria Clara Pessoa; Pereira, Tadeu José; Fonseca, Paulo Jorge; Santos, Carla Sousa; Costa, José Lino
The Lusitanian toadfish, Halobatrachus didactylus, like other batrachoidids, is a benthic fish species with nesting behaviour
during the breeding season. During this prolonged period it engages in mating activities and remains in the nest providing
parental care. It is not known whether males feed while providing parental care but it is likely that their limited mobility
may restrict their diet and influence their fitness. As a consequence, egg cannibalism could occur as a life-history strategy.
The aim of the present study is to ascertain the feeding behaviour of nesting males, in comparison to mature non-nesting
males, and to identify potential life-history traits related to egg...
Vieira, Manuel; Fonseca, Paulo Jorge; Amorim, Maria Clara Pessoa; Teixeira, Carlos J. C.
The study of acoustic communication in animals often requires not only the recognition of species specific acoustic signals but also the identification of individual subjects, all in a complex acoustic background. Moreover, when very long recordings are to be analyzed, automatic recognition and identification processes are invaluable tools to extract the relevant biological information. A pattern recognition methodology based on hidden Markov models is presented inspired by successful results obtained in the most widely known and complex acoustical communication signal: human speech. This methodology was applied here for the first time to the detection and recognition of fish acoustic signals,...
Campioni, Letizia; Granadeiro, José Pedro; Catry, Paulo
In long-lived species with slow maturation, prebreeders often represent a large percentage of the individuals alive at any moment, but
their ecology is still understudied. Recent studies have found prebreeding seabirds to differ in their isotopic (and trophic) niche from
adult breeders attending the same nesting colonies. These differences have been hypothesized to be linked to the less-developed foraging
performance of younger and less-experienced immatures or perhaps to their inferior competitive abilities. Such differences from
adults would wane as individuals mature (“the progressive ontogenetic shift hypothesis”) and could underpin the prolonged breeding
deferral until adulthood displayed by those species. This study documents a marked difference...
Pereira, Ana Martins; Cadeireiro, Emília Margarida; Robalo, Joana Isabel
Reproduction by sexual or asexual viviparity is a common
phenomenon in some anemone species. In this short
communication, the origin of the brooded young of Actinia equina
and A. schmidti from the Portuguese shore was investigated. DNA
was extracted from 56 brooding adult Actinia sp. and the nuclear
gene that codes for the 28S ribosomal subunit was sequenced.
Species identity was then assessed using GenBank. In total, 50
individuals were A. schmidti, five were A. equina and one had a
hybrid origin. Three adult anemones (the hybrid, one A. equina
and one A. schmidti) possessed two different 28S sequences and
so their offspring was selected for further analysis using the...
Costa, Bárbara Horta e; Claudet, Joachim; Franco, Gustavo; Erzini, Karim; Caro, Anthony; Gonçalves, Emanuel João
Dados suplementares associados com este artigo disponíveis na versão online em: http://dx.doi.org/10.1016/j.marpol.2016.06.021
Martins, Joana; Almada, Frederico; Gonçalves, Ana Sofia Ribeiro; Coelho, Pedro Duarte; Jorge, Paulo
The ability to be faithful to a particular area or site was analysed in the shanny Lipophrys pholis. Using passive integrated transponders, adults from a population of L. pholis at Cabo Raso, Portugal, were followed over a period of 3 years. The findings showed that site fidelity is a consistent behaviour during the breeding season with specific breeding males being found only in particular sectors within the area, and in specific nests throughout the years. The fact that, in general, L. pholis individuals were absent from the study area during the non-breeding season and breeding males were recorded returning to the...