López-Bernal, Álvaro; Morales, Alejandro; Garcia-Tejera, Omar; Testi, Luca; Orgaz, Francisco; Melo-Abreu, J.P.; Villalobos, Francisco J.
Dias, João; Alvarenga, Nuno; Sousa, Isabel
Santos, M.I.S.; Martins, S.R.; Pedroso, L.; Sousa, I.; Ferreira, M.A.S.S.
Abou-Mansour, Eliane; Débieux, Jean-Luc; Ramírez-Suero, Montserrat; Bénard-Gellon, Mélanie; Magnin-Robert, Maryline; Spagnolo, Alessandro; Chong, Julie; Farine, Sibylle; Bertsch, Christophe; L'Haridon, Floriane; Serrano, Mario; Fontaine, Florence; Rego, Cecilia; Larignon, Philippe
Liquid chromatography-diode array screening of the organic extract of the cultures of 13 isolates of the
fungus Neofusicoccum parvum, the main causal agent of botryosphaeria dieback of grapevine, showed
similar metabolites. One strain was selected for further chemical studies and led to the isolation and
characterisation of 13 metabolites. Structures were elucidated through spectroscopic analyses, including
one- and two-dimensional NMR and mass spectrometry, and through comparison to literature data. The
isolated compounds belong to four different chemical families: five metabolites, namely, ( )-terremutin
(1), (+)-terremutin hydrate (2), (+)-epi-sphaeropsidone (3) ( )-4-chloro-terremutin hydrate (4) and(+)-4-
hydroxysuccinate-terremutin hydrate (5), belong to the family of dihydrotoluquinones; two metabolites,
namely, (6S,7R) asperlin...
Danalache, Florina; Mata, Paulina; Martins, Margarida Moldão; Alves, Vitor D.
This work aimed the development of mango bars, made with fresh mango puree and gellan gum. The
influence of gellan concentration as well as the ratio of LA (low-acyl) and HA (high-acyl) gellan on the
rheological and microstructural properties of the mango bars was studied. Rheological outcomes showed
that both LA and HA, and in mixtures, were able to produce jellified products, within a rather low
maturation time. Furthermore, a synergistic effect was observed at different ratios of LA/HA, enabling the
production of mango bars with intermediate viscoelastic properties. Higher values of the dynamic
moduli were perceived as the LA content increased. Confocal microscopy is in...
Paredes, P.; Wei, Z.; Liu, Y.; Xu, D.; Xin, Y.; Zhang, B.; Pereira, L.S.
tFour years of soybean experimental data observed at Daxing, North China Plain, were used to assess theability of the AquaCrop model to predict soybean final biomass and yield. The model was parameterizedand calibrated using field data on leaf area index (LAI), available soil water, soil evaporation, biomass andfinal yield data. The model was assessed using calibrated and default parameters. Data on LAI were usedto derive the fraction of ground cover and to calibrate the green canopy cover (CC) curve. An accurate cal-ibration of the CC curve was performed, with low root mean square errors (RMSE < 7.3%). Results relativeto soil...
González, Maria G.; Ramos, Tiago B.; Carlesso, Reimar; Paredes, Paula; Petry, Mirta T.; Martins, Juliano D.; Aires, Núbia P.; Pereira, L.S.
Gama, Florinda; Saavedra, Teresa; Díaz, Isabel; Campillo, Maria del Carmen; Varennes, Amarilis de; Duarte, Amílcar; Pestana, Maribela; Correia, José Pedro
tThe absence of iron (Fe) in the nutrient solution induces several physiological and morphological adapta-tions in the roots of Poncirus trifoliata, a citrus rootstock, thereby modifying its overall nutritional status.Whether these changes are advantageous when plants are transplanted to calcareous soils needs to beassessed. To achieve this objective a two-phase experiment was established, first in nutrient solution(phase I) then in pots containing different soils (phase II). In phase I, P. trifoliata L. Raf. plants were grownin Hoagland’s solution with 120 M of Fe (Fe120 treatment) or without (Fe0 treatment). At the end ofphase I (87 days), Fe-chlorotic plants had less...
Oliveira, Ana; Alexandre, Elisabete M.C.; Coelho, Marta; Gomes, Maria Helena; Almeida, Domingos P.F.; Pintado, Manuela
The minimum loss of processed fruit nutritional quality must been guaranteed during storage and the
atmosphere can be a limiting step.
Strawberry pur ees flushed with gas mixtures: 10 kPa O2 þ 90 kPa N2, 100 kPa N2 and air (78 kPa
N2 þ 21 kPa O2 þ 0.03 kPa CO2) were stored for 90 days at 4 and 23 C and revealed no effect in total
antioxidant activity and in total phenolic content. The compounds (þ)-catechin, ( )-epicatechin and
quercetin-3-rutinoside were not affected by the atmospheres for both temperatures and ellagic acid was
the exception within strawberry phytochemicals, where its concentration was higher for samples...
Rodrigo, Isabel; Cristovão, Artur; Tibério, Manuel Luís; Baptista, Alberto; Maggione, Lara; Pires, Miguel
Study developed under the Rural Natework Project “Observatório de sistemas de produção localizados e valorização de produtos locais; Identificação, análise e divulgação de boas práticas de comercialização e valorização de produtos agro-alimentares tradicionais” (PNRR: PA 0400157-58-59)
Aybar, Vanessa E.; Melo-Abreu, J.P.; Searles, Peter S.; Matias, Angel C.; Del Rio, Carmen; Caballero, Juan M.; Rousseaux, M.Cecilia
Viegas, Inês; Santos, José Manuel Lima; Fontes, Magda Aguiar
Nas economias desenvolvidas, a segurança dos alimentos, o bem‑estar animal e o ambiente têm vindo a assumir relevância nas preocupações dos consumidores. Procurámos saber, através da metodologia dos grupos de discussão (focus groups) realizados em duas cidades de Portugal, Lisboa e Porto, as preocupações e perceções dos consumidores relativamente a estes atributos, os quais são essencialmente acreditados. Este conhecimento é fundamental para a aplicação posterior de métodos de preferências declaradas. Foi ainda possível obter, com base em exercícios de seleção entre carnes diferenciadas, e através de um modelo logit, intervalos de preços passíveis de serem utilizados posteriormente na definição de...
Domingos, Sara; Fino, Joana; Cardoso, Vânia; Sánchez, Claudia; Ramalho, José C.; Larcher, Roberto; Paulo, Octávio S.; Oliveira, Cristina M.; Goulão, Luis F.
Payer, R.; Figueiredo, E.; Mexia, A.
To evaluate an eventual parasitism and/or predation behaviour of the eulophid parasitoid Diglyphus isaea
(Walker, 1838) on Tuta absoluta (Meyrick, 1917) larvae two assays were performed releasing adult parasitoids. The
first assay was conducted in Petri dishes using tomato leaflets havig mines with T. absoluta larvae inside. The
second, was performed on previously infested potted tomato plants. In both assays no parasitism but only predation
was found. Predation was observed, mainly on L2 and L3 larvae which had moulted. Proportion of clearly preyed
larvae reached 17% in Petri dishes and 38% in potted plants. Predation was attested by the presence of punctures
done by the oviscapt...
Gomes, M.Helena; Vieira, Tiago; Fundo, Joana F.; Almeida, Domingos P.F.
tThe effect of pH, phenolic substrates, and food additives on polyphenoloxidase (PPO) activity and ontissue browning was studied in fresh-cut ‘Rocha’ pear. Substrates 4-methylcatechol, caffeic acid, (+)-catechin hydrate, catechol, chlorogenic acid, dopamine hydrochloride, and pyrogallol, were preparedin citric acid-phosphate buffer at pHs ranging from 3.0 to 8.0. pH optima for PPO activity dependedon the phenolic substrate. Activity was optimal at pH 5.0 for catechol and 4-methylcatechol; pH 6.0 forchlorogenic acid; pH 7.0 for dopamine, caffeic acid, and catechin; and pH 8.0 for pyrogallol. Discrepancieswere observed between the pH dependency of PPO activity and browning, as assessed by objective colormeasurement. Significant...
Leitão, Sara; Cerejeira, M. José; Van den Brink, Paul J.; Sousa, José Paulo
The potential terrestrial toxicity of three pesticides, azoxystrobin, chlorothalonil, and ethoprophos was
evaluated using reproduction ecotoxicological tests with different non-target species: the collembolan
Folsomia candida, the earthworm Eisenia andrei, and the enchytraeid Enchytraeus crypticus. All reproduction
tests were performed with natural soil from a Mediterranean agricultural area (with no pesticide
residues) in order to improve the relevance of laboratory data to field conditions. Controls were performed
with natural and standard artificial soil (OECD 10% OM). The fungicide azoxystrobin showed the
highest toxicity to earthworms (EC50 = 42.0 mg a.i. kg−1 dw soil). Collembolans were the most sensitive
taxa in terms of sublethal effects of chlorothalonil with an...
Santos, Erika S.; Magalhães, Maria Clara F.; Abreu, Maria Manuela; Macías, Felipe
Greenhouse pot experiments were conducted over 13 months to evaluate the effect of two amendment mixture
doses (30 and 75 Mg/ha) on the geochemical dispersion of trace elements by leaching hazardous mine wastes
from the São Domingos mine. Mineralogical evolution of these materials was also evaluated. Amendment mixtures
containing solid wastes from agriculture (plant remains + strawberry substrate and rockwool used for
the strawberry crop) and fromdistillation of Ceratonia siliqua L. and Arbutus unedo L. fruitswere used to improve
the chemical characteristics of leachates fromsulfide minewastes. Sulfide mine wastes had acidic characteristics,
aswell as high electrical conductivity and total element concentrations (g/kg; Al: 54.8–61.2; Fe: 104.0–110.0;...
Darouich, Hanaa M.; Pedras, Celestina M.G.; Gonçalves, José M.; Pereira, L.S.
Ramos, T.B.; Horta, A.; Gonçalves, M.C.; Martins, J.C.; Pereira, L.S.
Most pedotransfer functions (PTFs) have adopted soil texture information as the main predictor to estimate
soil hydraulic properties, whether inputs are defined in terms of the relative proportion of different grain size
particles or texture-based classifications. The objective of this studywas to develop ternary diagrams for estimating
soil water retention (θ) at−33 and−1500 kPa matric potentials, corresponding to the field capacity and wilting
point, respectively, from particle size distribution using two geostatistical approaches. The texture triangle was
divided into a 1% grid of soil texture composition resulting in 4332 different soil textures. Measured soil water
retention values determined in 742 soil horizons/layers located in Portugal were...
Costa, Raquel; Saraiva, Artur; Carvalho, Lopo; Duarte, Elizabeth
tThe use of plastic mulch films in agriculture had its beginning in the middle of last century andsince then its use has been intensified, with all the environmental problems related to their disposal.The biodegradable mulch films, which can be incorporated in the soil at the end of the crop cycle,appear as a possible solution for this problem. The goal of this work was to assess the performanceof five biodegradable mulch films when comparing to conventional polyethylene (PE), in strawberryproduction (Fragaria × ananassa Duch.). The field tests were performed for two years in the region ofRibatejo—Portugal. Taking into account that there...