Borges, Filipe; Gardner, Rui; Lopes, Telma; Calarco, Joseph P; Boavida, Leonor C; Slotkin, R; Martienssen, Robert A; Becker, Jörg D
The male germline in flowering plants differentiates by asymmetric division of haploid uninucleated
microspores, giving rise to a vegetative cell enclosing a smaller generative cell, which eventually undergoes a
second mitosis to originate two sperm cells. The vegetative cell and the sperm cells activate distinct genetic and epigenetic mechanisms to control pollen tube growth and germ cell specification, respectively. Therefore, a comprehensive characterization of these processes relies on efficient methods to isolate each of the different cell types throughout male gametogenesis.
We developed stable transgenic Arabidopsis lines and reliable purification tools based on
Fluorescence-Activated Cell Sorting (FACS) in order to isolate highly pure and...