Recursos de colección

E-Prints Complutense (100.312 recursos)

En este archivo los docentes e investigadores de la UCM depositan sus documentos en acceso abierto (open access)

Estado = Publicado

Mostrando recursos 1 - 20 de 861

  1. Cilastatin protects against cisplatin-induced nephrotoxicity without compromising its anticancer efficiency in rats

    Humanes, Blanca; Lázaro, Alberto; Camano, Sonia; Moreno Gordaliza, Estefanía; Lázaro, José A.; Blanco Codesido, Montserrat; Lara, José M.; Ortiz, Alberto; Gómez Gómez, M.Milagros; Martín Vasallo, Pablo; Tejedor, Alberto
    Cisplatin is an anticancer agent marred by nephrotoxicity; however, limiting this adverse effect may allow the use of higher doses to improve its efficacy. Cilastatin, a small molecule inhibitor of renal dehydropeptidase I, prevents proximal tubular cells from undergoing cisplatin-induced apoptosis in vitro. Here, we explored the in vivo relevance of these findings and the specificity of protection for kidney cells in cisplatin-treated rats. Cisplatin increased serum blood urea nitrogen and creatinine levels, and the fractional excretion of sodium. Cisplatin decreased the glomerular filtration rate, promoted histological renal injury and the expression of many pro-apoptotic proteins in the renal cortex,...

  2. LA-ICP-MS and nHPLC-ESI-LTQ-FT-MS/MS for the analysis of cisplatin–protein complexes separated by two dimensional gel electrophoresis in biological samples

    Moreno Gordaliza, Estefanía; Esteban Fernández, Diego; Giesen, Charlotte; Lehmann, Karola; Lázaro, Alberto; Tejedor, Alberto; Scheler, Christian; Cañas Montalvo, Benito; Jakubowski, Norbert; Linscheid, Michael W.; Gómez Gómez, M.Milagros
    A method for the analysis of Pt–protein complexes in biological samples, previously subjected to cisplatin treatment, has been developed. Proteins were separated by gel electrophoresis, and those bound to Pt were detected with high sensitivity by LA-ICP-(SF)-MS. Pt-containing spots were in-gel digested with trypsin, and the peptides produced identified using nHPLC-ESI-LTQ-FT-MS/MS. The influence of protein separation conditions, staining and gel processing prior to laser ablation on Pt–protein bonds preservation have been evaluated using standard proteins incubated with cisplatin. 2-DE separation under non-reducing conditions followed by either Coomassie blue brilliant or silver staining is appropriate for Pt–protein complexes, achieving a good...

  3. Estrategias bioanalíticas en estudios metalómicos de fármacos de platino

    Gómez Gómez, M.Milagros; Moreno Gordaliza, Estefanía; Mena Fernández, María Luz; Palacios Corvillo, M. Antonia; Cañas Montalvo, Benito
    En este artículo se muestra el potencial que presentan las modernas estrategias bioanalíticas en estudios metalómicos de fármacos de platino. La combinación de técnicas de separación multidimensionales cromatográficas y/o electroforéticas con la espectrometría de masas atómica ICP-MS y molecular ESI-MS/MS se presenta como una valiosa alternativa en este tipo de estudios.

  4. Cilastatin Attenuates Cisplatin-Induced Proximal Tubular Cell Damage

    Camano, Sonia; Lázaro, Alberto; Moreno Gordaliza, Estefanía; Torres, A.M.; Lucas, Carmen de; Humanes, Blanca; Lázaro, José A.; Gómez Gómez, M.Milagros; Bosca, Lisardo; Tejedor, Alberto
    A major area in cancer therapy is the search for protective strategies against cisplatin-induced nephrotoxicity. We investigated the protective effect of cilastatin on cisplatininduced injury to renal proximal tubular cells. Cilastatin is a specific inhibitor of renal dehydrodipeptidase I (DHP-I), which prevents hydrolysis of imipenem and its accumulation in the proximal tubule. Primary cultures of proximal cells were treated with cisplatin (1–30 M) in the presence or absence of cilastatin (200 g/ml). Apoptosis and mitochondrial injury were assessed by different techniques. Cisplatin uptake and DNA binding were measured by inductively coupled plasma spectrometry. HeLa cells were used to control the...

  5. Novel insights into the bottom-up mass spectrometry proteomics approach for the characterization of Pt-binding proteins: The insulin-cisplatin case study

    Moreno Gordaliza, Estefanía; Cañas Montalvo, Benito; Palacios Corvillo, M.Antonia; Gómez Gómez, M.Milagros
    The characterization of the interaction of platinum drugs with proteins has been previously performed using bottom-up proteomics approaches (enzymatic digestion followed by MS analysis). Nevertheless, the study of the stability of the Pt–protein bonds along the whole process has been obviated for the moment. Herein the suitability of the treatments implied during enzymatic digestion of Pt–protein adducts has been evaluated, focusing on the stability of the Pt bonds. Insulin–cisplatin adducts were generated in vitro and separated from unreacted cisplatin by HPLC, the separation being checked by HPLC-ICP-MS. The chromatographically isolated Pt–insulin adducts have been proved to resist overnight digestion including...

  6. Top-Down Mass Spectrometric Approach for the Full Characterization of Insulin-Cisplatin Adducts

    Moreno Gordaliza, Estefanía; Cañas Montalvo, Benito; Palacios Corvillo, M. Antonia; Gómez Gómez, M.Milagros
    The interaction of the antitumor drug cisplatin with insulin was studied using a top-down mass spectrometric approach. In vitro incubations were prepared under acidic and physiological conditions at different insulin/cisplatin molar ratios for different incubation times. Size exclusion chromatography-inductively coupled plasma mass spectrometry (SEC-ICPMS) analysis enabled the specific detection of platinum containing species attributed to the binding of the drug to the protein. Further analysis through matrix-assisted laser desorption ionization-timeof-flight mass spectrometry (MALDI-TOF MS) and nanoelectrospray ionization mass spectrometry using a linear ion trap (nESI-LIT-MS) allowed the identification of platinated mono-, di-, and even triadducts in the incubations. Platinum binding...

  7. Structural and lipid-binding characterization of human annexin A13a reveals strong differences with its long A13b isoform

    Fernández Lizarbe, Sara; Lecona, Emilio; Santiago Gómez, Angélica; Olmo, Nieves; Lizarbe, María Antonia; Turnay, Javier
    Annexin A13 is the founder member of the vertebrate family of annexins, which are comprised of a tetrad of unique conserved domains responsible for calcium-dependent binding to membranes. Its expression is restricted to epithelial intestinal and kidney cells. Alternative splicing in the N-terminal region generates two isoforms, A13a and A13b, differing in a deletion of 41 residues in the former. We have confirmed the expression of both isoforms in human colon adenocarcinoma cells at the mRNA and protein levels. We have cloned, expressed, and purified human annexin A13a for the first time to analyze its structural characteristics. Its secondary structure...

  8. Chiral Organic Dyes Endowed with Circularly Polarized Laser Emission

    Jiménez González, Josué; Cerdán Pedraza, Luis; Moreno Jiménez, Florencio; Maroto, Beatriz Lora; García Moreno, Inmaculada; Lunkley, Jamie L.; Muller, Gilles; Moya Cerero, Santiago de la
    The direct generation of efficient, tunable, and switchable circularly polarized laser emission (CPLE) would have far-reaching implications in photonics and material sciences. In this paper, we describe the first chiral simple organic molecules (SOMs) capable of simultaneously sustaining significant chemical robustness, high fluorescence quantum yields, and circularly polarized luminescence (CPL) ellipticity levels (|glum|) comparable to those of similar CPL-SOMs. All these parameters altogether enable efficient laser emission and CPLE with ellipticity levels 2 orders of magnitude stronger than the intrinsic CPL ones.

  9. Speeding up heterogeneous catalysis with an improved highly reusable catalyst for the preparation of enantioenriched secondary alcohols

    Sánchez Carnerero, Esther M. Márquez; Sandoval Torrientes, Rafael; Urieta Mora, Javier; Moreno Jiménez, Florencio; Maroto, Beatriz Lora; Moya Cerero, Santiago de la
    A new catalytic heterogeneous system, very efficient and highly reusable, for the preparation of enantioenriched secondary alcohols through the addition of diethylzinc to benzaldehyde has been developed. This system is based on a chiral bis(hydroxyamide) ligand supported on crosslinked polystyrene. The catalyst has been shown to be very efficient, leading to the corresponding secondary alcohol with an enantiomeric excess of 93% in a time as short as 2 h and using just 4% of the heterogeneous catalyst and just 1.5 equivalents of the organozinc reagent. We have demonstrated that the new catalyst is very stable and can be efficiently recycled...

  10. A Tourism Financial Conditions Index for Tourism Finance

    Chang, Chia-Lin; Hsu, Hui-Kuang; McAleer, Michael
    The paper uses monthly data on tourism related factors from April 2005 - June 2016 for Taiwan that applies factor analysis and Chang’s (2015) novel approach for constructing a tourism financial indicator, namely the Tourism Financial Conditions Index (TFCI). The TFCI is an adaptation and extension of the widely-used Monetary Conditions Index (MCI) and Financial Conditions Index (FCI) to tourism stock data. However, the method of calculation of the TFCI is different from existing methods of constructing the MCI and FCI in that the weights are estimated empirically. The empirical findings show that TFCI is statistically significant using the estimated...

  11. Structural and biomechanical corneal differences between patients suffering from primary congenital glaucoma and healthy volunteers

    Perucho González, Lucía; Sáenz Francés, Federico; Morales Fernández, Laura; Martínez de la Casa, Jose Maria; Méndez Hernández, Carmen D; Santos Bueso, Enrique; Brookes, John L.; García Feijoo, Julián
    Purpose: To determine whether a set of ocular morphometric and biomechanical variables are able to discriminate between healthy volunteers and patients suffering from primary congenital glaucoma (PCG). Methods: Case-control study in which 66 patients with PCG and 94 age-matched healthy subjects were evaluated using ocular response analyser (ORA) to record corneal biomechanical properties. Topographic corneal variables were obtained using the Pentacam in both groups. To determine the ability to discern between both groups, a multivariate binary logistic model was constructed. The outcome was the diagnosis of PCG and the predictors; the corneal variables analysed along with their first-term interactions. Sensitivity and...

  12. Search for photons with energies above 10(18) eV using the hybrid detector of the Pierre Auger Observatory

    Arqueros Martínez, Fernando; García Pinto, Diego; Minaya Flores, Ignacio Andrés; Vázquez Peñas, José Ramón; Otros, ...
    A search for ultra-high energy photons with energies above 1 EeV is performed using nine years of data collected by the Pierre Auger Observatory in hybrid operation mode. An unprecedented separation power between photon and hadron primaries is achieved by combining measurements of the longitudinal air-shower development with the particle content at ground measured by the fluorescence and surface detectors, respectively. Only three photon candidates at energies 1{2 EeV are found, which is compatible with the expected hadron-induced background. Upper limits on the integral flux of ultra-high energy photons of 0.027, 0.009, 0.008, 0.008 and 0.007 km(-2) sr(-1) yr(-1) are...

  13. Printing metal-spiked inks for LA-ICP-MS bioimaging internal standardization: comparison of the different nephrotoxic behavior of cisplatin, carboplatin, and oxaliplatin

    Moraleja San José, Irene; Esteban Fernández, Diego; Lázaro, Alberto; Humanes, Blanca; Neumann, Boris; Tejedor, Alberto; Mena, María Luz; Jakubowski, Norbert; Gómez Gómez, M.Milagros
    The study of the distribution of the cytostatic drugs cisplatin, carboplatin, and oxaliplatin along the kidney may help to understand their different nephrotoxic behavior. Laser ablation inductively coupled plasma mass spectrometry (LAICP-MS) allows the acquisition of trace element images in biological tissues. However, results obtained are affected by several variations concerning the sample matrix and instrumental drifts. In this work, an internal standardization method based on printing an Ir-spiked ink onto the surface of the sample has been developed to evaluate the different distributions and accumulation levels of the aforementioned drugs along the kidney of a rat model. A conventional...

  14. Reformas de los sistemas de pensiones. La necesidad de realizar modificaciones

    Vicente Merino, Ana
    En el presente artículo se abordan la situación de la Seguridad Social en España y diversas propuestas de reforma que se canalizan desde una acción básica: la modificación estructural de los niveles de protección.

  15. Bridging the gap between molecular and elemental mass spectrometry: Higher energy collisional dissociation (HCD) revealing elemental information

    Esteban Fernández, Diego; El-Khatib, Ahmed H.; Moraleja San José, Irene; Gómez Gómez, M.Milagros; Linscheid, Michael W.
    Molecular mass spectrometry has been applied to simultaneously obtain molecular and elemental information from metal-containing species. Energy tuning of the higher-energy collision dissociation (HCD) fragmentation cell allows the controlled production of typical peptide fragments or elemental reporter ions informing about the metallic content of the analyzed species. Different instrumental configurations and fragmentation techniques have been tested, and the efficiency extracting the elemental information has been compared. HCD fragmentation operating at very high energy led to the best results. Platinum, lanthanides, and iodine reporter ions from peptides interacting with cisplatin, peptides labeled with lanthanides-MeCAT-IA, and iodinated peptides, respectively, were obtained. The...

  16. Úlcera neurotrófica secundaria a aplasia de nervio trigémino en paciente con síndrome de Goldenhar

    Olavarri González, Gloria; García-Valcárcel González, Blanca; Baeza Autillo, Azucena; Balado Vázquez, Pilar
    Caso clínico: varón de 4 años de edad diagnosticado de síndrome de Goldenhar, sin antecedentes oftalmológicos relevantes, desarrolla una úlcera neurotrófica secundaria a aplasia de nervio trigémino que es tratada con trasplante de membrana amniótica multilaminar. Discusión: en el síndrome de Goldenhar no suele estar descrita la aplasia de nervio trigémino como manifestación oftalmológica típica. Por tanto, parece necesario realizar controles oftalmológicos rutinarios y desde una edad temprana, para evitar la aparición de complicaciones graves asociadas a la anestesia corneal.

  17. Signs and Symptoms of Dry Eye in Keratoconus Patients Before and After Intrastromal Corneal Rings Surgery

    Carracedo Rodríguez, Juan Gonzalo; Recchioni, Alberto; Alejandre Alba, Nicolás; Martín Gil, Alba; Batres Valderas, Laura; Jiménez Alfaro-Morote, Ignacio; Pintor, Jesús
    Purpose: Based on the relationship between keratoconus and dry eye, the aim of this study was to evaluate changes in signs and symptoms of dry eye in keratoconus patients before and after intrastromal corneal ring surgery. Methods: Fifteen keratoconus patients were enrolled in Fundación Jiménez-Díaz of Madrid and University Clinic of Optometry of the Universidad Complutense de Madrid (Madrid, Spain). Tear break up time (TBUT), Schirmer test without anesthesia, corneal staining, diadenosine tetraphosphate (Ap4A) concentration, and ocular surface disease index (OSDI) were evaluated. Impression cytology combined with laser confocal microscopy was performed to evaluate goblet cell density, mucin cloud height (MCH),...

  18. Signs and Symptoms of Dry Eye in Keratoconus Patients Before and After Intrastromal Corneal Rings Surgery

    Carracedo Rodríguez, Juan Gonzalo; Recchioni, Alberto; Alejandre Alba, Nicolás; Martín Gil, Alba; Batres Valderas, Laura; Jiménez Alfaro-Morote, Ignacio; Pintor, Jesús
    Purpose: Based on the relationship between keratoconus and dry eye, the aim of this study was to evaluate changes in signs and symptoms of dry eye in keratoconus patients before and after intrastromal corneal ring surgery. Methods: Fifteen keratoconus patients were enrolled in Fundación Jiménez-Díaz of Madrid and University Clinic of Optometry of the Universidad Complutense de Madrid (Madrid, Spain). Tear break up time (TBUT), Schirmer test without anesthesia, corneal staining, diadenosine tetraphosphate (Ap4A) concentration, and ocular surface disease index (OSDI) were evaluated. Impression cytology combined with laser confocal microscopy was performed to evaluate goblet cell density, mucin cloud height (MCH),...

  19. Current Perspectives on the Use of Anti-VEGF Drugs as Adjuvant Therapy in Glaucoma

    Andrés Guerrero, Vanessa; Perucho González, Lucía; García Feijoo, Julián; Morales Fernández, Laura; Sáenz Francés, Federico; Herrero Vanrell, Mª Rocío; Pablo Júlvez, Luis; Polo Llorens, Vicente; Martínez de la Casa, Jose Maria; Konstas, Anastasios Georgios P.
    The approval of one of the first anti-vascular endothelial growth factor (VEGF) agents for the treatment of neovascular age-related macular degeneration one decade ago marked the beginning of a new era in the management of several sight-threatening retinal diseases. Since then, emerging evidence has demonstrated the utility of these therapies for the treatment of other ocular conditions characterized by elevated VEGF levels. In this article we review current perspectives on the use of anti-VEGF drugs as adjuvant therapy in the management of neovascular glaucoma (NVG). The use of anti-VEGFs for modifying wound healing in glaucoma filtration surgery (GFS) is also...

  20. Normative database for separate inner retinal layers thickness using spectral domain optical coherence tomography in Caucasian population

    Nieves Moreno, María; Martínez de la Casa, Jose Maria; Cifuentes Canorea, Pilar; Sastre Ibáñez, Marina; Santos Bueso, Enrique; Sáenz Francés, Federico; Morales Fernández, Laura; García Feijoo, Julián
    Purpose: Develop the first normative database of the thickness of every inner retinal layer in the macular area in a healthy, Caucasian population between 18 to 87 years old, using Spectralis Optical Coherence Tomography (OCT). Methods: On this transversal, observational study, 300 patients between 18 to 87 years old and without an ophthalmological condition were recruited. Macular OCT scans were performed on all patients (Spectralis OCT, Heidelberg Engineering). An axial length measurement, and keratometry were performed using an optical biometer. The volume and thickness of the different macular sectors of the inner retinal layers (retinal nerve fiber layer (RNFL), ganglion cells...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.