González-Ballester, David; Sanz-Luque, Emanuel; Galván Cejudo, Aurora; Fernández Reyes, Emilio; Montaigu, Amaury de
Nitrogen assimilation and metabolism are essential processes for all living organisms, yet
there is still much to be learnt on how they are regulated. The use of Chlamydomonas reinhardtii
as a model system has been instrumental not only in identifying conserved regulation
mechanisms that control the nitrogen assimilation pathway, but also in understanding how
the intracellular nitrogen status regulates metabolic processes of industrial interest such as
the synthesis of biolipids. While the genetic regulators that control the nitrogen pathway are
successfully being unravelled, other layers of regulation have received less attention. Amino
acids, for example, regulate nitrogen assimilation in certain organisms, but their role in Chlamydomonas
La exposición a contaminantes es uno de los mayores retos a los que
debe hacer frente el mundo actual debido a los riesgos que implica, tanto para
los seres humanos como para la salud de los ecosistemas en general. Por
tanto, se necesitan potentes herramientas analíticas, fundamentalmente
ligadas a las respuestas biólogicas que desencadena la exposición a
contaminantes, para evaluar la calidad ambiental. Las metodologías ómicas,
junto con el uso de biomarcadores convencionales, han demostrado tener una
gran utilidad en este sentido, en estudios que usan al ratón moruno Mus
spretus como organismo bioindicador de contaminación ambiental en
ecosistemas terrestres del Parque Nacional de Doñana.
La complejidad de los ecosistemas...
Esteban, F.J.; Díaz, David; Hernández, Pilar; Caballero, J.A.; Dorado, G.; Gálvez, Sergio
The MC64-Cluster computer platform was designed, based on many-core CPU microprocessors: Tile64. MC64-Cluster architecture was outlined in terms of both hardware and software, including commands available to manage jobs and provided application programming interfaces to communicate and synchronize tiles, making this system easy to use. Massively, concurrent-searches of keys in B-trees, which are used in many applications, including bioinformatics, were used. Remarkable performance improvements were obtained when the cluster resources were combined with those available in host machine (hybrid or heterogeneous environments). These results were even more outstanding when analyzed in terms of performance-per-watt, highlighting their green-computing advantages. Together with...
Ayllón Egea, Leticia
In this Doctoral Thesis, garlic and olive-tree germplasm banks have been analyzed
by molecular markers. In short, the former has been traditionally used worldwide as a
common food ingredient and natural healing remedy in pharmacology/medicine. This is
ought to its interesting beneficial attributes, reducing high blood pressure, cholesterol and
atherosclerosis. Garlic also has preventive effects against cancer and antimicrobial
activity. On the other hand, olive-tree importance is undeniable. It is one of the most
cultivated species worldwide, especially in the Mediterranean basin, and it is the second
species for oil production after palm oil. The total area of cultivation is ten million
hectares. Its countless culinary and medicinal...
Casero, David; Cokus, Shawn; Pellegrini, Matteo; Merchant, Sabeeha S.; Grossman, Arthur R.; González-Ballester, David
The Chlamydomonas reinhardtii transcriptome was characterized from nutrient-replete and sulfur-depleted wild-type and
snrk2.1 mutant cells. This mutant is null for the regulatory Ser-Thr kinase SNRK2.1, which is required for acclimation of the
alga to sulfur deprivation. The transcriptome analyses used microarray hybridization and RNA-seq technology. Quantitative
RT-PCR evaluation of the results obtained by these techniques showed that RNA-seq reports a larger dynamic range of
expression levels than do microarray hybridizations. Transcripts responsive to sulfur deprivation included those encoding
proteins involved in sulfur acquisition and assimilation, synthesis of sulfur-containing metabolites, Cys degradation, and
sulfur recycling. Furthermore, we noted potential modifications of cellular structures during sulfur deprivation, including...
Pootakham, Wirulda; Mus, Florence; Yang, Wenqiang; Catalanotti, Claudia; Magneschi, Leonardo; Montaigu, Amaury de; Higuera, Jose J.; Prior, Matthew; Galván Cejudo, Aurora; Fernández Reyes, Emilio; Grossman, Arthur R.; González-Ballester, David
A method was developed to identify insertional mutants of Chlamydomonas reinhardtii disrupted for selected
target genes. The approach relies on the generation of thousands of transformants followed by PCR-based
screenings that allow for identification of strains harboring the introduced marker gene within specific genes of
interest. Our results highlight the strengths and limitations of two independent screens that differed in the nature
of the marker DNA used (PCR-amplified fragment containing the plasmid-free marker versus entire linearized
plasmid with the marker) and in the strategies used to maintain and store transformants.