Recursos de colección

Repositorio Institucional de la Universidad de Cordoba :: Spain (54.987 recursos)

El repositorio recoge todo tipo de materiales digitales: artículos de revistas, comunicaciones a congresos, tesis doctorales, documentos de trabajo, materiales docentes y objetos de aprendizaje, así como los productos digitales del patrimonio bibliográfico de la Universidad de Córdoba.

Mostrando recursos 1 - 4 de 4

  1. Arginine is a component of the ammonium- CYG56 signalling cascade that represses genes of the nitrogen assimilation pathway in Chlamydomonas reinhardtii

    González-Ballester, David; Sanz-Luque, Emanuel; Galván Cejudo, Aurora; Fernández Reyes, Emilio; Montaigu, Amaury de
    Nitrogen assimilation and metabolism are essential processes for all living organisms, yet there is still much to be learnt on how they are regulated. The use of Chlamydomonas reinhardtii as a model system has been instrumental not only in identifying conserved regulation mechanisms that control the nitrogen assimilation pathway, but also in understanding how the intracellular nitrogen status regulates metabolic processes of industrial interest such as the synthesis of biolipids. While the genetic regulators that control the nitrogen pathway are successfully being unravelled, other layers of regulation have received less attention. Amino acids, for example, regulate nitrogen assimilation in certain organisms, but their role in Chlamydomonas has not...
    (application/pdf) - 04-may-2018

  2. MC64-Cluster: Many-Core CPU Cluster Architecture and Performance Analysis in B-Tree Searches

    Esteban, F.J.; Díaz, David; Hernández, Pilar; Caballero, J.A.; Dorado, G.; Gálvez, Sergio
    The MC64-Cluster computer platform was designed, based on many-core CPU microprocessors: Tile64. MC64-Cluster architecture was outlined in terms of both hardware and software, including commands available to manage jobs and provided application programming interfaces to communicate and synchronize tiles, making this system easy to use. Massively, concurrent-searches of keys in B-trees, which are used in many applications, including bioinformatics, were used. Remarkable performance improvements were obtained when the cluster resources were combined with those available in host machine (hybrid or heterogeneous environments). These results were even more outstanding when analyzed in terms of performance-per-watt, highlighting their green-computing advantages. Together with...
    (application/pdf) - 21-feb-2018

  3. RNA-Seq Analysis of Sulfur-Deprived Chlamydomonas Cells Reveals Aspects of Acclimation Critical for Cell Survival

    Casero, David; Cokus, Shawn; Pellegrini, Matteo; Merchant, Sabeeha S.; Grossman, Arthur R.; González-Ballester, David
    The Chlamydomonas reinhardtii transcriptome was characterized from nutrient-replete and sulfur-depleted wild-type and snrk2.1 mutant cells. This mutant is null for the regulatory Ser-Thr kinase SNRK2.1, which is required for acclimation of the alga to sulfur deprivation. The transcriptome analyses used microarray hybridization and RNA-seq technology. Quantitative RT-PCR evaluation of the results obtained by these techniques showed that RNA-seq reports a larger dynamic range of expression levels than do microarray hybridizations. Transcripts responsive to sulfur deprivation included those encoding proteins involved in sulfur acquisition and assimilation, synthesis of sulfur-containing metabolites, Cys degradation, and sulfur recycling. Furthermore, we noted potential modifications of cellular structures during sulfur deprivation, including...
    (application/pdf) - 03-may-2018

  4. Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants

    Pootakham, Wirulda; Mus, Florence; Yang, Wenqiang; Catalanotti, Claudia; Magneschi, Leonardo; Montaigu, Amaury de; Higuera, Jose J.; Prior, Matthew; Galván Cejudo, Aurora; Fernández Reyes, Emilio; Grossman, Arthur R.; González-Ballester, David
    A method was developed to identify insertional mutants of Chlamydomonas reinhardtii disrupted for selected target genes. The approach relies on the generation of thousands of transformants followed by PCR-based screenings that allow for identification of strains harboring the introduced marker gene within specific genes of interest. Our results highlight the strengths and limitations of two independent screens that differed in the nature of the marker DNA used (PCR-amplified fragment containing the plasmid-free marker versus entire linearized plasmid with the marker) and in the strategies used to maintain and store transformants.
    (application/pdf) - 03-may-2018

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