Recursos de colección

Hokkaido University Collection of Scholarly and Academic Papers (130.505 recursos)

HUSCAP (Hokkaido University Collection of Scholarly and Academic Papers) contains peer-reviewed journal articles, proceedings, educational resources and any kind of scholarly works of Hokkaido University.

Volume 60, Number 2&3

Mostrando recursos 1 - 10 de 10

  1. INFORMATION: Theses for the Bachelor of Veterinary Medicine

    Hokkaido University conferred the degree of Bachelor of Veterinary Medicine to the following 44 graduates of the School of Veterinary Medicine on March 22, 2012.

  2. INFORMATION: Theses for the degree of Doctor of Philosophy (on June 29, 2012)

    Hokkaido University conferred the degree of Doctor of Philosophy on June 29, 2012 to 3 recipients.

  3. INFORMATION: Theses for the degree of Doctor of Philosophy (on March 22, 2012)

    Hokkaido University conferred the degree of Doctor of Philosophy on March 22, 2012 to 18 recipients.

  4. Bovine viral diarrhea virus infection in a dairy herd with high prevalence of persistently infected calves

    Helal, Mahmoud Atef Youssef; Okamatsu, Hiroyuki; Tajima, Motoshi
    A dairy herd including approximately 50 milking cows and 40 heifers and calves was investigated. This herd was detected with high prevalence of calves persistently infected (PI) with bovine viral diarrhea virus (BVDV). Nine PI animals including a milking cow and 8 newborn calves were detected in the herd within 4 months. Prevalence of PI animals in this herd was estimated 7.0% which was very high compared to that estimated in previous reports. All newborn PI calves were strongly suspected to have a single origin of infection as estimated from the homology of the virus genes. The cause of high...

  5. Plasmodium circumflexum in a Shikra (Accipiter badius): Phylogeny and ultra-structure of the haematozoa

    Salakij, Jarernsak; Lertwatcharasarakul, Preeda; Kasorndorkbua, Chaiyan; Salakij, Chaleow
    A wild-caught, juvenile Shikra (Accipiter badius) was evaluated for rehabilitation at the Kasetsart University Raptor Rehabilitation Unit (KURRU) with a history of weakness. Plasmodium sp. was observed by both light and electron microscopy in blood obtained on day 1 of evaluation. Based on the appearance of erythrocytic meronts and gametocytes, the parasites were defined as Plasmodium (Giovannolaia) circumflexum. The sequence analysis of the mitochondrial cytochrome b gene from the plasmodia was closely related to parasites found in the Grey-headed woodpecker from Myanmar and the Brown hawk-owl from Singapore. Transmission electron microscopic examination revealed organelles in the haematozoa and heterophils that...

  6. The chicken 2'-5' oligoadenylate synthetase A inhibits the replication of West Nile Virus

    Tag-El-Din-Hassan, Hassan T.; Sasaki, Nobuya; Moritoh, Kanako; Torigoe, Daisuke; Maeda, Akihiko; Agui, Takashi
    West Nile virus (WNV) is a pathogen to cause West Nile encephalitis when the infection occurs in the brain. Previous studies in mice identified the 2'-5' oligoadenylate synthetase 1b (Oas1b) gene as a determining factor for resistance to WNV infection. In addition, it has been suggested that human OAS1 and OASL are associated with the resistance to the WNV infection. WNV is maintained in nature through a complex life cycle involving wild birds and mosquitoes. Birds are not only susceptible to the WNV, but also act as reservoir hosts, thus participating in the spread of the disease. It has previously...

  7. Development and evaluation of indirect enzyme-linked immunosorbent assay for a screening test to detect antibodies against classical swine fever virus

    Sakoda, Yoshihiro; Wakamoto, Hiroaki; Tamura, Tehpin; Nomura, Takushi; Naito, Michiko; Aoki, Hiroshi; Morita, Hiroshi; Kida, Hiroshi; Fukusho, Akio
    An indirect enzyme-linked immunosorbent assay (ELISA) was developed for a screening test to detect antibodies against classical swine fever virus (CSFV). Viral glycoproteins, which were purified from swine kidney cells infected with CSFV ALD/A76 strain by the immunoaffinity purification using monoclonal antibody against E2 protein, were adsorbed on a microtiter plate as the antigen for the antibody detection. Each antibody titer of serum sample was expressed as a sample per positive value calculated with optical absorbance of each sample and that of a positive control. The advantage of this ELISA is its higher sensitivity: most sera containing more than 4...

  8. Inhibitory effects of an M2-specific monoclonal antibody on different strains of influenza A virus

    Muto, Nilton Akio; Yoshida, Reiko; Suzuki, Tadaki; Kobayashi, Shintaro; Ozaki, Hiroichi; Fujikura, Daisuke; Manzoor, Rashid; Muramatsu, Mieko; Takada, Ayato; Kimura, Takashi; Sawa, Hirofumi
    New approaches to the treatment of influenza have been designed based on the highly conserved antigenicity of the M2 envelope protein among influenza A virus strains. The present study examined the anti-viral activities of an anti-M2 ectodomain (M2e) monoclonal antibody (clone rM2ss23), which binds to the M2 proteins of the influenza A virus strains A/Aichi/2/68 (H3N2) (Aichi) and A/PR/8/34 (H1N1) (PR8). The results showed that rM2ss23 bound to both Aichi and PR8 M2 proteins expressed on the cell surface. While the antibody did not prevent virus entry into cells, it significantly inhibited plaque formation by the Aichi strain in a...

  9. Effects of interferon-τ on cattle persistently infected with bovine viral diarrhea virus

    Kohara, Junko; Nishikura, Yumiko; Konnai, Satoru; Tajima, Motoshi; Onuma, Misao
    In this study, the antiviral effects of bovine interferon-τ (boIFN-τ) on bovine viral diarrhea virus (BVDV) were examined in vitro and in vivo. In the in vitro experiments, the replication of cytopathic and non-cytopathic BVDV was inhibited in the bovine cells treated with boIFN-τ. The replication of BVDV was completely suppressed by boIFN-τ at a concentration higher than 10^[2] U/ml. In order to examine the effect of boIFN-τ on virus propagation in cattle persistently infected (PI) with non-cytopathic BVDV, boIFN-τ was subcutaneously administered to PI cattle at 10^[5] U/kg or 10^[6] U/kg body weight 5 times per week for 2...

  10. Analysis of energy generation and glycolysis pathway in diminazene aceturate-resistant Babesia gibsoni isolate in vitro

    Rajapakshage, Bandula Kumara Wickramasekara; Yamasaki, Masahiro; Murakami, Masahiro; Tamura, Yu; Lim, Sue Yee; Osuga, Tatsuyuki; Sasaki, Noboru; Nakamura, Kensuke; Ohta, Hiroshi; Takiguchi, Mitsuyoshi
    In our previous study, the level of parasitemia of the diminazene aceturate (DA)-resistant B. gibsoni isolate was continuously lower than that of the wild-type, indicating the possible alteration of energy metabolism in that isolate. Therefore, in the present study, the concentrations of ATP, glucose, lactate, and pyruvate, and the activities of lactate dehydrogenase and pyruvate kinase in the wild-type and DA-resistant isolate of B. gibsoni were measured and compared to investigate the amount of energy generation and the activity of the glycolysis pathway. As a result, the intracellular ATP and glucose concentrations in the DA-resistant B. gibsoni isolate were significantly...

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