Recursos de colección

Repositorio Hipermedial UNR - Repositorio Hipermedial de la Universidad Nacional de Rosario (6.314 recursos)

El Repositorio Hipermedial UNR es un repositorio académico abierto creado para archivar, preservar y distribuir digitalmente en variados formatos tanto materiales de enseñanza y aprendizaje (objetos de aprendizaje) como la producción científica de Investigación y Desarrollo (I+D) de los profesores, profesional es e investigadores de la UNR.

Mostrando recursos 1 - 7 de 7

  1. Preparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity

    Ruiz Álvarez, Jimena Inés; Teijeiro, Juan Manuel; Marini, Patricia Estela
    Immunological methods involving pregnancy associated glycoproteins (PAG) are used for cattle pregnancy detection. The faults of these methods could be overcomed by using antibodies specific for each member of the PAG family. In order to differentiate between very similar proteins, preparation of antibodies specific for peptides is a method of choice. In this work, we summarize a series of considerations regarding peptide design and choose free access NCBI, Antigenicity Plot, EMBOSS Antigenic and Expasy tools to apply them. We design peptides specific for different reported PAG members and obtain the corresponding polyclonal antibodies for five of them.

  2. Annexin A2 and S100A10 in the mammalian oviduct

    Teijeiro, Juan Manuel; Roldán, María Lorena; Marini, Patricia Estela
    In many mammals, upon entry into the female reproductive tract, a subpopulation of sperm is stored in the oviduct forming a functional reservoir. In the oviducts of pig and cow, Annexin A2 (AnxA2) has been linked to the binding of sperm. This protein may exist as a monomer or bound to S100A10 and both forms are associated with different biological functions. S100A10 has not yet been reported in the oviduct. The objective of this work is to analyze for the presence of S100A10 in the oviduct and to advance the study of AnxA2 and S100A10 in this organ. This work shows the presence of both proteins, AnxA2 and S100A10, in...

  3. Protein kinase C activity in boar sperm

    Teijeiro, Juan Manuel; Marini, Patricia Estela; Bragado, María Julia; García-Marin, Luis
    Male germ cells undergo different processes within the female reproductive tract to successfully fertilize the oocyte. These processes are triggered by different extracellular stimuli leading to activation of protein phosphorylation. Protein kinase C (PKC) is a key regulatory enzyme in signal transduction mechanisms involved in many cellular processes. Studies in boar sperm demonstrated a role for PKC in the intracellular signaling involved in motility and cellular volume regulation. Experiments using phorbol 12-myristate 13-acetate (PMA) showed increases in the Serine/Threonine phosphorylation of substrates downstream of PKC in boar sperm. In order to gain knowledge about those cellular processes regulated by PKC, we evaluate the effects of...

  4. Protein kinase C activity in boar sperm

    Teijeiro, Juan Manuel; Marini, Patricia Estela; Bragado, María Julia; García-Marin, Luis
    Male germ cells undergo different processes within the female reproductive tract to successfully fertilize the oocyte. These processes are triggered by different extracellular stimuli leading to activation of protein phosphorylation. Protein kinase C (PKC) is a key regulatory enzyme in signal transduction mechanisms involved in many cellular processes. Studies in boar sperm demonstrated a role for PKC in the intracellular signaling involved in motility and cellular volume regulation. Experiments using phorbol 12-myristate 13-acetate (PMA) showed increases in the Serine/Threonine phosphorylation of substrates downstream of PKC in boar sperm. In order to gain knowledge about those cellular processes regulated by PKC, we evaluate the effects of...

  5. Use of Assisted Reproduction Techniques in Male Pathologies: Molecule Based Sperm Selection Methods

    Marini, Patricia Estela; Munuce, María José; Caille, Adriana María; Zumoffen, Carlos; Teijeiro, Juan Manuel
    Infertility is a widespread problem that is found in over 15% of couples. In 50 % of the cases, the male factor is the key. In order to improve pregnancy rates in assisted reproductive techniques (ART), new methods to select sperm subpopulations are required. These methods are under constant evaluation, and their possible genetic and epigenetic effects on the offspring must be considered. New concepts such as DNA fragmentation and sperm apoptosis are being introduced in andrology and are considered to develop new techniques. Recently, molecule-based methods have been developed for the purpose of overcoming deficiencies in classical selection methods...

  6. Use of Assisted Reproduction Techniques in Male Pathologies: Molecule Based Sperm Selection Methods

    Marini, Patricia Estela; Munuce, María José; Caille, Adriana María; Zumoffen, Carlos; Teijeiro, Juan Manuel
    Infertility is a widespread problem that is found in over 15% of couples. In 50 % of the cases, the male factor is the key. In order to improve pregnancy rates in assisted reproductive techniques (ART), new methods to select sperm subpopulations are required. These methods are under constant evaluation, and their possible genetic and epigenetic effects on the offspring must be considered. New concepts such as DNA fragmentation and sperm apoptosis are being introduced in andrology and are considered to develop new techniques. Recently, molecule-based methods have been developed for the purpose of overcoming deficiencies in classical selection methods...

  7. Use of Annexin V based Sperm Selection in Assisted Reproduction

    Teijeiro, Juan Manuel; Munuce, María José; Caille, Adriana María; Zumoffen, Carlos; Marini, Patricia Estela
    Innovative methods to select sperm subpopulations with the best fertilizing ability are needed in assisted reproductive techniques (ART) in order to improve fertilization and pregnancy rates, while also considering possible epigenetic effects on the offspring. Molecular based selection methods are searched for, under the premise that they could be an improvement over classical selection by morphology and movement. One of these methods sustains the elimination of sperm that can bind to annexin A5 (ANX V), coupled to paramagnetic beads, through the phosphatidyl-serine exposed on their membranes upon apoptosis. Although reports accumulate about the use of this method, controversy persists as to the benefits of ANX...

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