ETD at Indian Institute of Science
(529 recursos)
Repository of Theses and Dissertations of Indian Institute of Science, Bangalore, India. The repository has been developed to capture, disseminate and preserve research theses of Indian Institute of Science.
Mostrando recursos 1 - 14 de 14
1.
Mechanism Of mom Gene Transactivation By Transcription Factor C Of Phage MU - Chakraborty, Atanu
Regulation of transcription initiation is the major determining event employed by the cell to control gene expression and subsequent cellular processes. The weak promoters, with low basal transcription activities, are activated by activators. Bacteriophage Mu mom gene, which encodes a unique DNA modification function, is detrimental to cell when expressed early or in large quantities. Mu has designed a complex, well-controlled and orchestrated regulatory network for mom expression to ensure its synthesis only in late lytic cycle. The phage encoded transcription activator protein C activates the gene by promoter unwinding of the DNA and thereby recruiting of RNAP to the...
2.
Functional Characterization Of The Saccharomyces Cerevisiae Splicing Factor, Prp17 In pre-mRNA Splicing And Cell Cycle Progression: An Analysis Through Global Expression Profiling, Protein Interactions And Spliceosomal Associations - Katoch, Aparna
The presence of introns in all the eukaryotic genomes identified so far underscores the fundamental and ubiquitous role of pre-mRNA splicing. The spliceosomal machinery, comprised of five small nuclear RNAs and several protein factors, catalyzes the two-transesterification reactions of splicing with precision and consistency. Through a complex network of protein-protein and RNA-protein interactions it ensures the removal of the intron and ligation of the flanking exons to yield the mature mRNA.
Prpl7 is a splicing factor that functions at the second-step of splicing (Vijayraghavan et all, 1989). Null alleles of prpl7 are viable at 23°C but die at temperatures above 33°C...
3.
Molecular Characterisation Of The ATP Binding Cassette (ABC) Transporter Type FtsE And FtsX Proteins Of Mycobacterium Tuberculosis - Mir, Mushtaq Ahmad
Mycobacterium tuberculosis, the principal causative agent of tuberculosis (TB) in
humans, is considered to be a successful pathogen owing to the elicitation of multidrug resistance, ability to survive inside macrophage phagosomes by taking nutrients from host cell cytoplasm, and the capacity to alternate between proliferating and dormant (nonproliferating) conditions of growth. Thus, whether one looks at tubercle bacillus from the standpoint of regulation of cell division in the host system, or uptake of nutrients from the host cell cytoplasm or elicitation of drug resistance, the requirement for ATP Binding Cassette (ABC) transporter type protein complexes, which might be involved in the...
4.
Role Of Matrix Protein Of Rinderpest Virus In Viral Morphogenesis - Subhashri, R
Rinderpest virus is an enveloped Nonsegmented Negative Stranded RNA Virus (NNSV) belonging to the genus Morbillivirus in the Family Paramyxoviridae and the causative organism for cattle plague. The virion has a transport component and a replication component. The transport component consists of a lipid membrane
with two external membrane-anchored glycoproteins, namely Hemagglutinin (H) and
Fusion (F) proteins that are necessary for cell entry and release of newly formed virus
particles. The replication component consists of viral genomic RNA encapsidated by the nucleoprotein (N) and a RNA polymerase complex (Large subunit L and
phosphoprotein P). These two components are linked together by the...
5.
Transcriptional Analysis Of The Principal Cell Division Gene ftsZ Of Mycobacterium Tuberculosis And Mycobacterium Smegmatis - Roy, Sougata
The success of Mycobacterium tuberculosis as a pathogen is due to its remarkable
ability to: (i). adapt to and survive inside activated macrophages under nonproliferating condition, (ii). put up drug resistance and (iii). enter into hypoxia-induced dormancy and remain in nonproliferating condition, be resistant to drugs, and get reactivated into
proliferation when favourable conditions arise. Thus, regulation of cell division (arrest and resumption) is an obligatory event that is critical to the pathogen for the establishment of successful infection, latency and reactivation process in human host. Therefore, in order to understand and combat the successful survival strategy of the bacterium inside...
6.
Diversity In Indian Equine Rotaviruses And Structure And Function Of Rotavirus Non Structural Protein 4 (NSP4) - Deepa, R
Rotaviruses, members of the family Reoviridae, are the major etiologic agents of severe, acute dehydrating diarrhea in the young of many mammalian species, including humans, calves and foals. Recent estimates indicate an annual death toll of approximately 600,000 infants due to rotavirus, besides inflicting staggering
economic burden worldwide. Most of these deaths occur in the developing countries
and India is estimated to account for about a quarter of these deaths. Extensive
molecular epidemiology studies carried out by our laboratory have revealed many
interesting aspects about rotavirus diversity in this country.
Molecular epidemiology of rotaviruses causing severe diarrhea in foals in two
organized farms in northern...
7.
Notch1-Induced Survival Signaling And Its Implications In Cancer Therapeutics - Mungamuri, Sathish Kumar
Notch receptors and ligands are type I transmembrane proteins that regulate
development and differentiation during cell-cell contact. There are four Notch receptor
homologues and five notch ligands, identified in humans till date. Upon ligand activation, Notch1 intracellular domain (NIC-1) is released into the cytoplasm, which binds to several proteins as well as translocates into the nucleus to effect the Notch signaling. In the absence of the activated Notch signaling, the Notch target genes are kept repressed by the transcriptional repressor C protein binding factor 1 (CBF1) also known as RBPjk or CSL for CBF1/Su(H)/Lag1. RBPjk binds to the sequence “CGTGGGAA”...
8.
Breast Cancer Susceptibility Gene 1 (BRCA1) And Breast Cancer - Lakhotia, Smita
Breast Cancer susceptibility gene 1 (BRCA1) & Breast Cancer
Breast cancer is one of the most common malignancies affecting women worldwide. About 5-10% of all cases are estimated to be familial. Mutations in the BRCA1 (Breast Cancer susceptibility gene 1) gene account for about 15-20% of inherited breast cancer cases and 60-80% of families predisposed to both breast and ovarian cancer. BRCA1 mutations also result in susceptibility to early-onset breast and ovarian cancer. The human BRCA1 gene encodes a multi-domain 1,863 amino acid nuclear protein that is expressed in a wide variety of adult human tissues. The N-terminal end of BRCA1...
9.
Studies On Initiator tRNA Selection On The Ribosomes In Escherichia Coli - Das, Gautam
The studies reported in this thesis address the aspects of initiator tRNA selection in Escherichia Coli. A summary of the relevant literature discussing the process of ptotein biosynthesis in general and initiator tRNA selection, in particular is presented in chapter 1. The next chapter (Chapter2) describes the ‘Materials and Methods’ used throughout the experimental work carried out in this thesis. It is followed by two chapters(Chapter 3 and Chapter 4) which describe the isolation and characterization of an E. coli mutant, to understand the mechanism of initiator tRNA selection. Chapter 5 comprises of some experimental work and future perspectives on...
10.
Role Of Idiotypic Anti-Idiotypic Network In The Sustenance Of Immunological Memory - Gangadhar, Vidya
Living amidst a milieu of pathogenic organisms, vertebrates are in constant threat of contracting one or the other disease. As a mechanism of protection against such ‘invasions’, the vertebrate immune system has evolved to serve two main functions. One, to generate a specific immune response against the invading pathogen (in the from of specific antibodies and cell mediated immune responses). And two, to ‘remember’ the pathogen after the first exposure and mount a heightened and quicker immune response upon subsequent encounters. This phenomenon is called immunological memory, or anamnestic response and is achieved by the generation of memory B and...
11.
Transcriptional Regulation And The Role Of Galactose Metabolism In The Virulence Of Candida Albicans - Singh, Vijender
Candida albicans, a commensal of gastrointestinal and uro-vaginal tract can cause superficial as well as life threatening disseminated infections under conditions of lowered immunity of the host such as HIV infection, drug induced immune suppression [given during organ transplantation to prevent rejection] and radiation therapy [head and neck
cancer patients] (Odds, 1988; Fidel and Sobel, 1996). Candida albicans shows a range of morphologies, it can switch from budding yeast morphology to pseudohyphae (chains of elongated cells with visible constrictions at the sites of septa) and hyphae (linear filaments without visible constrictions at the septa) (Mitchell, 1998). The various factors that...
12.
Metabolism Of Queuosine, A Modified Nucleoside, In Escherichia Coli And Caenorhabditis Elegans And Dual Function Of Bovine Mitochondrial Initiation Factor 2 As Initiation Factors 1 And 2 In Escherichia Coli - Gaur, Rahul
The studies reported in this thesis address firstly, the biology of a modified nucleoside, Queuosine (Q) and secondly, the properties of mitochondrial translation initiation factor 2. A summary of the relevant literature on both these topics is presented in Chapter 1. Section I of this ‘General Introduction’ summarizes the literature on biosynthesis and physiological importance of Queuosine. Section II is a brief review of the current understanding of translation initiation in Eubacteria. Information about the mitochondrial translation initiation apparatus also features as a subsection. The next chapter (Chapter 2), describes the ‘Materials and Methods’ used throughout the experimental work presented...
13.
Regulation Of Interferon Regulatory Factor-2 mRNA Translation By 'IRES' Element : Possible Role Of trans Acting Factors - Dhar, Debojyoti
Cellular response to various stress conditions involves regulation of gene expression by different mechanisms. Translation is the final step in the flow of genetic information and regulation at this level allows an early response to changes in physiological conditions. Initiation of translation is the rate-limiting step of protein synthesis and hence is tightly regulated. Translation initiation in mammalian cells is mainly by “cap dependent pathway” wherein the 5’methyl guanosine “cap” structure is recognized by certain canonical initiation factors along with 40S ribosomal subunit and the complex scans the 5’UTR till it recognizes initiator AUG. This leads to the joining of...
14.
Rinderpest Virus Transcription : Functional Dissection Of Viral RNA Polymerase And Role Of Host Factor Ebp1 In Virus Multiplication - Gopinath, M
Rinderpest virus (RPV) belongs to the order Mononegavirale which comprises non segmented negative sense RNA viruses including human pathogens such as Measles, Ebola and Marburg virus. RPV is the causative agent of Rinderpest disease in large ruminants, both domesticated and wild. The viral genome contains a non segmented negative sense RNA encapsidated by nucleocapsid protein (N-RNA). Viral transcription/replication is carried out by the virus encoded RNA dependent RNA polymerase represented by the large protein L and phosphoprotein P as (L-P) complex. Viral transcription begins at the 3’ end of the genome 3’le-N-P-M-F-H-N-tr-5’ with the synthesis of 55nt leader RNA followed...
1.
