Recursos de colección

ETD at Indian Institute of Science (2.518 recursos)

Repository of Theses and Dissertations of Indian Institute of Science, Bangalore, India. The repository has been developed to capture, disseminate and preserve research theses of Indian Institute of Science.

Microbiology and Cell Biology (mcbl)

Mostrando recursos 1 - 20 de 88

  1. Study Of Rpb4, A Component Of RNA Polymerase II As A Coordinator Of Transcription Initiation And Elongation In S. Cerevisiae

    Deshpande, Swati
    RNA polymerase II (Pol II) is the enzyme responsible for the synthesis of all mRNAs in eukaryotic cells. As the central component of the eukaryotic transcription machinery, Pol II is the final target of transcription regulatory pathways. While the role for different Pol II associated proteins, co-activators and general transcription factors (GTFs) in regulation of transcription in response to different stimuli is well studied, a similar role for some subunits of the core Pol II is only now being recognized. The studies reported in this thesis address the role of the fourth largest subunit of Pol II, Rpb4, in transcription...

  2. Fidelity Of Translation Initiation In E. coli : Roles Of The Transcription-recycling Factor RapA, 23S rRNA Modifications, And Evolutionary Origin Of Initiator tRNA

    Bhattacharyya, Souvik

  3. Molecular Characterisation Of Mycobacterium Tuberculosis Fic Protein And Its Gene And Identification And Characterisation Of A Novel Functional Interaction Between FtsZ And NDK in Mycobacteria

    Mishra, Saurabh
    Living organisms employ different kinds of mechanisms, to regulate the functions of genes or their products, which may help in maintaining homeostasis inside the cell or may help in fighting hostile environment in the case of pathogenic organisms. These mechanisms act at the transcriptional, post-transcriptional, translational, and post-translational levels. In order to understand the physiology of an organism, it is essential to obtain an in-depth knowledge of such mechanisms, in which several proteins participate in interlinked pathways. In this regard, the present study focuses on two such proteins: (i). the newly identified Fic (Filamentation induced by cAMP) protein; and (ii)....

  4. Studies On Phosphorylation And Oligomerization Of Rotavirus Nonstructural Protein 5 (NSP5) And Cellular Pathways That Regulate Virus Replication

    Namsa, Nima Dondu
    Rotavirus is one of the leading etiological agents of gastroenteritis in young of many species including humans worldwide and is responsible for about 600,000 infant deaths per annum. Rotavirus belongs to the Reoviridae family, and its genome is composed of 11 double-stranded RNA segments that encode six structural proteins and six nonstructural proteins. Rotavirus replication is fully cytoplasmic and occurs within highly specialized regions called viroplasms. NSP2 and NSP5 have been shown to be essential for viroplasm formation and, when co-expressed in uninfected cells, to form viroplasm¬like structures. A recent study suggest a key role for NSP5 in architectural assembly...

  5. Mechanistic And Functional Insights Into Mycobacterium Bovis BCG Triggered TLR2 Signaling : Implications For Immune Evasion Strategies

    Ghorpade, Devram Sampat
    Mycobacteria are multifaceted pathogens capable of causing both acute disease as well as an asymptomatic latent infection. Host immune responses during mycobacterial infection involve potent cell effector functions including that of CD4+, CD8+ and γδT cells, macrophages and dendritic cells (DCs). Further, the critical regulators of protective immunity to mycobacterial infection include IFN-γ, IL-12, IL-23, TNF-α, lymphotoxins, CD40, nitric oxide and reactive oxygen species. However, the success of mycobacterial infection often relies in its ability to evade immune surveillance mechanisms mediated by sentinels of host immunity by modulating host signal transduction pathways and expression of immunoregulatory molecules. Therefore, the key...

  6. Transcription In Mycobacteria : From Initiation To Elongation

    China, Arnab
    The global re-emergence of TB and other mycobacterial infections have underscored the need for a thorough investigation of the biology of the causative agent, Mycobacterium tuberculosis, at the molecular level. The peculiar features of the bacterium such as slow growth rate, dormancy, unique cell wall composition and resistance towards phagocytosis by macrophages demands a detailed understanding of different essential molecular processes including transcription in this genus. Sequencing of several mycobacterial genomes provided an impetus for understanding the gene function and regulation of this formidable pathogen. Transcriptional regulation is one of the major mechanisms controlling gene expression. While a number of...

  7. Studies On The Mechanism Of Uracil Excision Repair In Escherichia Coli And Structure-Function Relationship Of Single Stranded DNA Binding Proteins From Escherichia Coli And Mycobacterium Tuberculosis

    Bharti, Sanjay Kumar
    To maintain the genomic integrity, cell has evolved various DNA repair pathways. Base Excision Repair pathway (BER) is one such DNA repair pathway which is dedicated to protect DNA from small lesions such as oxidation, alkylation, deamination and loss of bases. Uracil is a promutagenic base which appears in the genome as a result of misincorporation of dUTP or due to oxidative deamination of cytosine. Uracil-DNA glycosylases (UDGs) are DNA repair enzymes that initiate multistep base excision repair (BER) pathway to excise uracil from DNA. Excision of uracil generates an abasic site (APDNA). AP-sites are cytotoxic and mutagenic to the...

  8. Insights Into The Contribution Of Hfq In Salmonella Pathogenesis : Possible Role In Immune Evasion And Vaccine Development

    Allam, Uday Sankar
    Chapter I Introduction Salmonellae are facultative Gram-negative intracellular pathogens. Different serovars of it causes a variety of diseases in multiple hosts with different disease outcomes. Salmonella enterica serovar Enteritidis and Typhimurium (STM) can infect domestic animals causing gastroenteritis or typhoid like fever. Typhoid fever in humans which is actually caused by Salmonella enterica serovar Typhi still remains a significant health problem in many parts of the world with an estimated annual incidence of nearly 16 million cases and about 600,000 deaths. The infection begins via the orofecal route following which it invades the intestinal mucosa through several ways, namely by...

  9. Mechanistic And Functional Insights Into Mycobacterium Bovis BCG Induced Expression Of Cyclooxygenase-2 : Implications For Immune Evasion Strategies

    Bansal, Kushagra
    Mycobacteria are multifaceted pathogens capable of causing both acute disease as well as an asymptomatic latent infection. Protective immunity against pathogenic mycobacteria depends principally on cell-mediated immunity executed by efficient anti-infectious functions of type 1 T helper (Th1) subset of CD4+ T cells. The polarization of Th1 responses is orchestrated by IL-12 secreted by antigen presenting cells (APCs) such as macrophages and dendritic cells (DCs). A hallmark of Th1 type CD4+ T cells is the production of IFN-γ that activates plethora of innate cell-mediated immunity. It is well known that cytokines such as IFN-γ, IL-12 and TNF-α are required for...

  10. Identification Of GAL102 Encoded UDP-Glucose 4, 6 Dehydratase Activity, As A Novel Virulence Factor In Candida Albicans

    Sen, Manimala
    Among fungal pathogens responsible for opportunistic infections, species of the genus Candida have a major role (Mitchell, 1998). Various Candida species cause superficial infections which can be cured by the currently available antifungal arsenal (Noble and Johnson, 2007). However, species of the genus Candida are also responsible for life-threatening systemic infections, particularly in immunocompromised patients with weakened immune system. Among Candida species, C. albicans, which can also be a commensal of the skin and the gastrointestinal and genitourinary tracts, is responsible for the majority of Candida bloodstream infections. However, there is an increasing incidence of infections caused by C. glabrata...

  11. Modulation Of Bacterial Pathogenesis By Curcumin

    Marathe, Sandhya
    Foodborne diseases are one among the diseases with high morbidity and mortality rate. The concern is raised with the emergence of pathogenic strains that are resistant to the available set of antibiotics. Conventional regimens fail to treat the infections caused by these pathogens prolonging the sickness leading to increased morbidity and mortality. The situation can get further complicated with the dietary intake of the host. Of late it has been understood that the dietary flavonoids play an important role in regulating the immune system. Curcumin, a pigment from turmeric, is one among such bioflavonoid with an immunomodulatory potential. Curcumin has...

  12. Host-Pathogen Interaction Between Staphylococcus Aureus And Murine Macrophages

    Ananthalakshmi, T K
    Chapter 1: Introductionn Staphylococci are gram positive rotund bacteria that grow in clusters; and hence get their name. The genus of Staphylococcus comprises of over 30 species of which S. epidermidis and S.aureus are significant in their interaction with humans and are known to cause diseases. S.aureus invades various soft tissues and causes a vast multitude of diseases spanning from simple boils and abscesses to osteomyelitis and endocarditis, which can become fatal upon the onset of bacteremia and toxic shock. S. aureus has also been established as one of the leading causes of nosocomial infections especially because of their multi-drug resistant...

  13. Delineation Of Signaling Events Regulating Mycobacterium Bovis BCG Induced Expression Of MMR-9 And SPI6 : Possible Implications For Immune Subversion Mechanisms

    Kapoor, Nisha
    One key to the pathogenic potential of the mycobacteria lies in their capacity to resist destruction by infected macrophages and dendritic cells. Robust host immune responses during mycobacterial infection often involve a potent CD4, CD8 and gamma delta T cell mediated effector responses including lysis of mycobacteria infected host cells, secretion of variety of cytokines like IFN-γ etc. However, pathogenic mycobacteria survives for prolonged periods in the phagasomes of infected macrophages within the host in an asymptomatic, latent state and can reactivate years later if the host’s immune system wanes. One of the most devastating consequences of infection with mycobactreia...

  14. Role Of Cis Acting RNA Elements In Internal Initiation Of Translation Of Coxsackievirus B3 RNA

    Bhattacharyya, Sankar

  15. Differential Expression Of tRNA1 Gly Genes From Within A Multigene Family In Bombyx Mori

    Parthasarthy, Akhila

  16. Grass Floret Organ Specification And Differentiation : Molecular-Genetic And Evolutionary Studies With Rice As A Model System

    Sriram, P

  17. Functional Analysis Of AtCAP Genes In Arabidopsis Thaliana

    Morakhia Anand, R

  18. Characterization Of A Bovine Rotavirus From Humans And Studies On The Structural And Biological Properties Of Rotaviral Enterotoxigenic Nonstructural Protein 4

    Jagannath, M R

  19. Characterization Of A Novel Genotype Rotavirus And Investigations On Signalling Pathways In Rotavirus Infected MA104 Cells

    Reddy, Yugandhar B S

  20. Perpetuation Of T cell Memory : A Role For Anti-Idiotypic T Cells

    Lal, Girdhari

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