Mostrando recursos 201 - 220 de 4.535

  1. Obtención y caracterización de pectinas modificadas mediante tratamientos químicos y físicos

    Muñoz-Almagro, Nerea
    Foro para la presentación y discusión de los Trabajos de Fin de Máster realizados por los alumnos del Máster de Química Agrícola y Nuevos Alimentos del curso 2014-2015.-- Directores: Francisco Javier Moreno Andújar y María del Mar Villamiel Guerra. Departamento de Bioactividad y Análisis de Alimentos. Instituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM).

  2. Percepción de las mujeres en situación de dependencia química dentro de Mato Grosso, Brasil

    Nascimento, Vagner Ferreira do; Moll, Marciana Fernandes; Lemes, Alisséia Guimarães; Cabral, Juliana Fernandes; Cardoso, Tayane Próspero; Luis, Margarita Antonia Villar
    Objetivou conhecer a percepção de mulheres em situação de dependência química no interior de Mato Grosso. Trata-se de estudo descritivo-exploratório, qualitativo, fundamentado na Teoria de Peplau. Realizado na única comunidade terapêutica feminina, pertencente a Região Nordeste Matogrossense - Brasil, no período de março a abril de 2016, com 15 mulheres. A coleta de dados ocorreu no próprio serviço, utilizando entrevista semiestruturada com roteiro e gravação. Para análise, utilizouse a Análise de Conteúdo. Os resultados revelaram que as mulheres em tratamento nessa comunidade, possuem experiências semelhantes ligadas às drogas, mas tendem a responder de forma distinta ao tratamento, principalmente por que...

  3. Percepción de las mujeres en situación de dependencia química dentro de Mato Grosso, Brasil

    Nascimento, Vagner Ferreira do; Moll, Marciana Fernandes; Lemes, Alisséia Guimarães; Cabral, Juliana Fernandes; Cardoso, Tayane Próspero; Luis, Margarita Antonia Villar
    Objetivou conhecer a percepção de mulheres em situação de dependência química no interior de Mato Grosso. Trata-se de estudo descritivo-exploratório, qualitativo, fundamentado na Teoria de Peplau. Realizado na única comunidade terapêutica feminina, pertencente a Região Nordeste Matogrossense - Brasil, no período de março a abril de 2016, com 15 mulheres. A coleta de dados ocorreu no próprio serviço, utilizando entrevista semiestruturada com roteiro e gravação. Para análise, utilizouse a Análise de Conteúdo. Os resultados revelaram que as mulheres em tratamento nessa comunidade, possuem experiências semelhantes ligadas às drogas, mas tendem a responder de forma distinta ao tratamento, principalmente por que...

  4. Cilastatin protects against cisplatin-induced nephrotoxicity without compromising its anticancer efficiency in rats

    Humanes, Blanca; Lázaro, Alberto; Camano, Sonia; Moreno Gordaliza, Estefanía; Lázaro, José A.; Blanco Codesido, Montserrat; Lara, José M.; Ortiz, Alberto; Gómez Gómez, M.Milagros; Martín Vasallo, Pablo; Tejedor, Alberto
    Cisplatin is an anticancer agent marred by nephrotoxicity; however, limiting this adverse effect may allow the use of higher doses to improve its efficacy. Cilastatin, a small molecule inhibitor of renal dehydropeptidase I, prevents proximal tubular cells from undergoing cisplatin-induced apoptosis in vitro. Here, we explored the in vivo relevance of these findings and the specificity of protection for kidney cells in cisplatin-treated rats. Cisplatin increased serum blood urea nitrogen and creatinine levels, and the fractional excretion of sodium. Cisplatin decreased the glomerular filtration rate, promoted histological renal injury and the expression of many pro-apoptotic proteins in the renal cortex,...

  5. Cilastatin protects against cisplatin-induced nephrotoxicity without compromising its anticancer efficiency in rats

    Humanes, Blanca; Lázaro, Alberto; Camano, Sonia; Moreno Gordaliza, Estefanía; Lázaro, José A.; Blanco Codesido, Montserrat; Lara, José M.; Ortiz, Alberto; Gómez Gómez, M.Milagros; Martín Vasallo, Pablo; Tejedor, Alberto
    Cisplatin is an anticancer agent marred by nephrotoxicity; however, limiting this adverse effect may allow the use of higher doses to improve its efficacy. Cilastatin, a small molecule inhibitor of renal dehydropeptidase I, prevents proximal tubular cells from undergoing cisplatin-induced apoptosis in vitro. Here, we explored the in vivo relevance of these findings and the specificity of protection for kidney cells in cisplatin-treated rats. Cisplatin increased serum blood urea nitrogen and creatinine levels, and the fractional excretion of sodium. Cisplatin decreased the glomerular filtration rate, promoted histological renal injury and the expression of many pro-apoptotic proteins in the renal cortex,...

  6. LA-ICP-MS and nHPLC-ESI-LTQ-FT-MS/MS for the analysis of cisplatin–protein complexes separated by two dimensional gel electrophoresis in biological samples

    Moreno Gordaliza, Estefanía; Esteban Fernández, Diego; Giesen, Charlotte; Lehmann, Karola; Lázaro, Alberto; Tejedor, Alberto; Scheler, Christian; Cañas Montalvo, Benito; Jakubowski, Norbert; Linscheid, Michael W.; Gómez Gómez, M.Milagros
    A method for the analysis of Pt–protein complexes in biological samples, previously subjected to cisplatin treatment, has been developed. Proteins were separated by gel electrophoresis, and those bound to Pt were detected with high sensitivity by LA-ICP-(SF)-MS. Pt-containing spots were in-gel digested with trypsin, and the peptides produced identified using nHPLC-ESI-LTQ-FT-MS/MS. The influence of protein separation conditions, staining and gel processing prior to laser ablation on Pt–protein bonds preservation have been evaluated using standard proteins incubated with cisplatin. 2-DE separation under non-reducing conditions followed by either Coomassie blue brilliant or silver staining is appropriate for Pt–protein complexes, achieving a good...

  7. LA-ICP-MS and nHPLC-ESI-LTQ-FT-MS/MS for the analysis of cisplatin–protein complexes separated by two dimensional gel electrophoresis in biological samples

    Moreno Gordaliza, Estefanía; Esteban Fernández, Diego; Giesen, Charlotte; Lehmann, Karola; Lázaro, Alberto; Tejedor, Alberto; Scheler, Christian; Cañas Montalvo, Benito; Jakubowski, Norbert; Linscheid, Michael W.; Gómez Gómez, M.Milagros
    A method for the analysis of Pt–protein complexes in biological samples, previously subjected to cisplatin treatment, has been developed. Proteins were separated by gel electrophoresis, and those bound to Pt were detected with high sensitivity by LA-ICP-(SF)-MS. Pt-containing spots were in-gel digested with trypsin, and the peptides produced identified using nHPLC-ESI-LTQ-FT-MS/MS. The influence of protein separation conditions, staining and gel processing prior to laser ablation on Pt–protein bonds preservation have been evaluated using standard proteins incubated with cisplatin. 2-DE separation under non-reducing conditions followed by either Coomassie blue brilliant or silver staining is appropriate for Pt–protein complexes, achieving a good...

  8. Estrategias bioanalíticas en estudios metalómicos de fármacos de platino

    Gómez Gómez, M.Milagros; Moreno Gordaliza, Estefanía; Mena Fernández, María Luz; Palacios Corvillo, M. Antonia; Cañas Montalvo, Benito
    En este artículo se muestra el potencial que presentan las modernas estrategias bioanalíticas en estudios metalómicos de fármacos de platino. La combinación de técnicas de separación multidimensionales cromatográficas y/o electroforéticas con la espectrometría de masas atómica ICP-MS y molecular ESI-MS/MS se presenta como una valiosa alternativa en este tipo de estudios.

  9. Estrategias bioanalíticas en estudios metalómicos de fármacos de platino

    Gómez Gómez, M.Milagros; Moreno Gordaliza, Estefanía; Mena Fernández, María Luz; Palacios Corvillo, M. Antonia; Cañas Montalvo, Benito
    En este artículo se muestra el potencial que presentan las modernas estrategias bioanalíticas en estudios metalómicos de fármacos de platino. La combinación de técnicas de separación multidimensionales cromatográficas y/o electroforéticas con la espectrometría de masas atómica ICP-MS y molecular ESI-MS/MS se presenta como una valiosa alternativa en este tipo de estudios.

  10. Cilastatin Attenuates Cisplatin-Induced Proximal Tubular Cell Damage

    Camano, Sonia; Lázaro, Alberto; Moreno Gordaliza, Estefanía; Torres, A.M.; Lucas, Carmen de; Humanes, Blanca; Lázaro, José A.; Gómez Gómez, M.Milagros; Bosca, Lisardo; Tejedor, Alberto
    A major area in cancer therapy is the search for protective strategies against cisplatin-induced nephrotoxicity. We investigated the protective effect of cilastatin on cisplatininduced injury to renal proximal tubular cells. Cilastatin is a specific inhibitor of renal dehydrodipeptidase I (DHP-I), which prevents hydrolysis of imipenem and its accumulation in the proximal tubule. Primary cultures of proximal cells were treated with cisplatin (1–30 M) in the presence or absence of cilastatin (200 g/ml). Apoptosis and mitochondrial injury were assessed by different techniques. Cisplatin uptake and DNA binding were measured by inductively coupled plasma spectrometry. HeLa cells were used to control the...

  11. Cilastatin Attenuates Cisplatin-Induced Proximal Tubular Cell Damage

    Camano, Sonia; Lázaro, Alberto; Moreno Gordaliza, Estefanía; Torres, A.M.; Lucas, Carmen de; Humanes, Blanca; Lázaro, José A.; Gómez Gómez, M.Milagros; Bosca, Lisardo; Tejedor, Alberto
    A major area in cancer therapy is the search for protective strategies against cisplatin-induced nephrotoxicity. We investigated the protective effect of cilastatin on cisplatininduced injury to renal proximal tubular cells. Cilastatin is a specific inhibitor of renal dehydrodipeptidase I (DHP-I), which prevents hydrolysis of imipenem and its accumulation in the proximal tubule. Primary cultures of proximal cells were treated with cisplatin (1–30 M) in the presence or absence of cilastatin (200 g/ml). Apoptosis and mitochondrial injury were assessed by different techniques. Cisplatin uptake and DNA binding were measured by inductively coupled plasma spectrometry. HeLa cells were used to control the...

  12. Novel insights into the bottom-up mass spectrometry proteomics approach for the characterization of Pt-binding proteins: The insulin-cisplatin case study

    Moreno Gordaliza, Estefanía; Cañas Montalvo, Benito; Palacios Corvillo, M.Antonia; Gómez Gómez, M.Milagros
    The characterization of the interaction of platinum drugs with proteins has been previously performed using bottom-up proteomics approaches (enzymatic digestion followed by MS analysis). Nevertheless, the study of the stability of the Pt–protein bonds along the whole process has been obviated for the moment. Herein the suitability of the treatments implied during enzymatic digestion of Pt–protein adducts has been evaluated, focusing on the stability of the Pt bonds. Insulin–cisplatin adducts were generated in vitro and separated from unreacted cisplatin by HPLC, the separation being checked by HPLC-ICP-MS. The chromatographically isolated Pt–insulin adducts have been proved to resist overnight digestion including...

  13. Novel insights into the bottom-up mass spectrometry proteomics approach for the characterization of Pt-binding proteins: The insulin-cisplatin case study

    Moreno Gordaliza, Estefanía; Cañas Montalvo, Benito; Palacios Corvillo, M.Antonia; Gómez Gómez, M.Milagros
    The characterization of the interaction of platinum drugs with proteins has been previously performed using bottom-up proteomics approaches (enzymatic digestion followed by MS analysis). Nevertheless, the study of the stability of the Pt–protein bonds along the whole process has been obviated for the moment. Herein the suitability of the treatments implied during enzymatic digestion of Pt–protein adducts has been evaluated, focusing on the stability of the Pt bonds. Insulin–cisplatin adducts were generated in vitro and separated from unreacted cisplatin by HPLC, the separation being checked by HPLC-ICP-MS. The chromatographically isolated Pt–insulin adducts have been proved to resist overnight digestion including...

  14. Top-Down Mass Spectrometric Approach for the Full Characterization of Insulin-Cisplatin Adducts

    Moreno Gordaliza, Estefanía; Cañas Montalvo, Benito; Palacios Corvillo, M. Antonia; Gómez Gómez, M.Milagros
    The interaction of the antitumor drug cisplatin with insulin was studied using a top-down mass spectrometric approach. In vitro incubations were prepared under acidic and physiological conditions at different insulin/cisplatin molar ratios for different incubation times. Size exclusion chromatography-inductively coupled plasma mass spectrometry (SEC-ICPMS) analysis enabled the specific detection of platinum containing species attributed to the binding of the drug to the protein. Further analysis through matrix-assisted laser desorption ionization-timeof-flight mass spectrometry (MALDI-TOF MS) and nanoelectrospray ionization mass spectrometry using a linear ion trap (nESI-LIT-MS) allowed the identification of platinated mono-, di-, and even triadducts in the incubations. Platinum binding...

  15. Corrección ionosférica de observaciones GNSS para zonas de latitud geomagnética baja apoyada en la observación de señales VLF

    Barona Mendoza, Jhon Jairo
    Esta investigación, busca generar un aporte en el conocimiento de la ionósfera colombiana, entendiendo que la localización de Colombia dentro de la zona de anomalía ecuatorial, hace que se presenten dificultades en el desarrollo de actividades que involucran el uso de ondas electromagnéticas y tecnologías satelitales. La propagación de radio señales en esta región, se ve afectada por su interacción con la ionosfera sin poder corregir plenamente estos efectos debido a que los modelos existentes para esto, no dan respuesta adecuada a los diferentes fenómenos que alteran su comportamiento de manera aleatoria y prácticamente impredecible. En el trabajo se desarrolla...

  16. Diseño de una planta de gasificación con cogeneración para el aprovechamiento energético de la cascarilla de arroz en un proceso industrial

    Sánchez Lario, Alejandro
    En el presente proyecto se pretende comprobar y demostrar la viabilidad de un sistema de cogeneración usando biomasa gasificada como combustible. Dicho sistema se instalará en una planta de producción de arroz, cuyo proceso de fabricación genera como residuo la cascarilla de arroz y demanda energía tanto térmica como eléctrica. La obtención de la energía mecánica necesaria se hará mediante un motor de combustión interna alternativo, especialmente preparado para funcionar con gas de síntesis. Este gas se obtendrá mediante la gasificación de la biomasa producida en la propia planta: la cascarilla de arroz. La cascarilla de arroz es un tejido vegetal constituido por celulosa y sílice, elementos que...

  17. Estudio de nuevos hormigones ecoeficientes. Reología y comportamiento

    Agudo de Pablo, Raquel
    Durante los últimos años, los medios de comunicación, los Gobiernos y las asociaciones ecologistas, entre otros, han advertido a la sociedad de un hecho importante: la velocidad a la que los seres humanos están consumiendo los recursos es superior a la velocidad que la Tierra es capaz de volver a producirlos, una práctica totalmente insostenible. Bien es cierto que a partir de finales de los 70, se empezaron a establecer medidas legislativas que pretendían eliminar o reducir el impacto ambiental producido. No obstante, las industrias y empresas tenían una actitud reactiva ante ellas, sólo tomando medidas cuando eran sancionadas. De...

  18. Printing metal-spiked inks for LA-ICP-MS bioimaging internal standardization: comparison of the different nephrotoxic behavior of cisplatin, carboplatin, and oxaliplatin

    Moraleja San José, Irene; Esteban Fernández, Diego; Lázaro, Alberto; Humanes, Blanca; Neumann, Boris; Tejedor, Alberto; Mena, María Luz; Jakubowski, Norbert; Gómez Gómez, M.Milagros
    The study of the distribution of the cytostatic drugs cisplatin, carboplatin, and oxaliplatin along the kidney may help to understand their different nephrotoxic behavior. Laser ablation inductively coupled plasma mass spectrometry (LAICP-MS) allows the acquisition of trace element images in biological tissues. However, results obtained are affected by several variations concerning the sample matrix and instrumental drifts. In this work, an internal standardization method based on printing an Ir-spiked ink onto the surface of the sample has been developed to evaluate the different distributions and accumulation levels of the aforementioned drugs along the kidney of a rat model. A conventional...

  19. Bridging the gap between molecular and elemental mass spectrometry: Higher energy collisional dissociation (HCD) revealing elemental information

    Esteban Fernández, Diego; El-Khatib, Ahmed H.; Moraleja San José, Irene; Gómez Gómez, M.Milagros; Linscheid, Michael W.
    Molecular mass spectrometry has been applied to simultaneously obtain molecular and elemental information from metal-containing species. Energy tuning of the higher-energy collision dissociation (HCD) fragmentation cell allows the controlled production of typical peptide fragments or elemental reporter ions informing about the metallic content of the analyzed species. Different instrumental configurations and fragmentation techniques have been tested, and the efficiency extracting the elemental information has been compared. HCD fragmentation operating at very high energy led to the best results. Platinum, lanthanides, and iodine reporter ions from peptides interacting with cisplatin, peptides labeled with lanthanides-MeCAT-IA, and iodinated peptides, respectively, were obtained. The...

  20. Exploring the Application of the Negishi Reaction of HaloBODIPYs: Generality, Regioselectivity, and Synthetic Utility in the Development of BODIPY Laser Dyes

    Palao Utiel, Eduardo; Durán Sampedro, Gonzalo; Moya Cerero, Santiago de la; Madrid, Miriam; García López, Carmen; Rodríguez Agarrabeitia, Antonia; Verbelen, Bram; Dehaen, Wim; Boens, Nöel; Ortíz García, María Josefa
    The generality of the palladium-catalyzed C–C coupling Negishi reaction when applied to haloBODIPYs is demonstrated on the basis of selected starting BODIPYs, including polyhalogenated and/or asymmetrical systems, and organozinc reagents. This reaction is an interesting synthetic tool in BODIPY chemistry, mainly because it allows a valuable regioselective postfunctionalization of BODIPY chromophores with different functional groups. In this way, functional patterns that are difficult to obtain by other procedures (e.g., asymmetrically functionalized BODIPYs involving halogenated positions) can now be made. The regioselectivity is achieved by controlling the reaction conditions and is based on almost-general reactivity preferences, and the nature of the...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.