Mostrando recursos 1 - 20 de 1.135

  1. Diseño de un sistema de monitorización del reflujo gástrico mediante espectrofotometría en radiofrecuencia.

    SERRADOR VALLCANERA, ALBA
    [ES] Debido a que en los últimos años se ha observado un número creciente de casos de trastorno por Reflujo Gastroesofágico, así como de las enfermedades interrelacionadas con este (p.e. hernia de hiato), el desarrollo de técnicas no invasivas de detección de ácido clorhídrico por la vía esofágica puede ser de gran interés científico y social. El Reflujo Gástrico consiste en un complejo desorden de motricidad al cual lo acompañan síntomas tales como: sensación de ardor retroesternal, dolor abdominal, regurgitación gástrica o sabor amargo. En algunos casos puede desembocar en complicaciones graves como la esofagitis erosiva (daños severos en el esófago). En la actualidad, el diagnóstico puede...

  2. Regulation of proteasome activity by P2Y2 receptor underlies the neuroprotective effects of extracellular nucleotides

    Diego García, Laura de; Ramírez Escudero, Mercedes; Sebastián Serrano, Álvaro; Díaz Hernández, Juan Ignacio; Pintor, Jesús; Lucas Lozano, José J.; Díaz Hernández, Miguel
    The Ubiquitin-Proteasome System (UPS) is essential for the regulation of the cellular proteostasis. Indeed, it has been postulated that an UPS dysregulation is the common mechanism that underlies several neurological disorders. Considering that extracellular nucleotides, through their selective P2Y2 receptor (P2Y2R), play a neuroprotective role in various neurological disorders that course with an UPS impairment, we wonder if this neuroprotective capacity resulted from their ability to modulate the UPS. Using a cellular model expressing two different UPS reporters, we found that the stimulation of P2Y2R by its selective agonist Up4U induced a significant reduction of UPS reporter levels. This reduction...

  3. Regulation of proteasome activity by P2Y2 receptor underlies the neuroprotective effects of extracellular nucleotides

    Diego García, Laura de; Ramírez Escudero, Mercedes; Sebastián Serrano, Álvaro; Díaz Hernández, Juan Ignacio; Pintor, Jesús; Lucas Lozano, José J.; Díaz Hernández, Miguel
    The Ubiquitin-Proteasome System (UPS) is essential for the regulation of the cellular proteostasis. Indeed, it has been postulated that an UPS dysregulation is the common mechanism that underlies several neurological disorders. Considering that extracellular nucleotides, through their selective P2Y2 receptor (P2Y2R), play a neuroprotective role in various neurological disorders that course with an UPS impairment, we wonder if this neuroprotective capacity resulted from their ability to modulate the UPS. Using a cellular model expressing two different UPS reporters, we found that the stimulation of P2Y2R by its selective agonist Up4U induced a significant reduction of UPS reporter levels. This reduction...

  4. Changes in P2Y Purinergic Receptor Expression in the Ciliary Body in a Murine Model of Glaucoma

    Fonseca Vázquez, Begoña; Martínez Águila, Alejandro; Pérez de Lara, María Jesús; Miras Portugal, María Teresa; Gómez Villafuertes, María Rosa; Pintor, Jesús
    Glaucoma is a neuropathology, often accompanied by an elevated intraocular pressure (IOP), which can lead to blindness. Since DBA/2J mice develop glaucoma, several studies of the physiopathology of glaucoma have been reported in this animal model. It is also known that purinergic receptors are involved in the pathology of glaucoma by controlling aqueous humor production and drainage and therefore controlling IOP. There are no studies on purinergic receptors in the DBA/2J model of glaucoma and their relation to the development of the pathology, so the aim of this study was to make an approach to the purinergic mechanisms involved in...

  5. Changes in P2Y Purinergic Receptor Expression in the Ciliary Body in a Murine Model of Glaucoma

    Fonseca Vázquez, Begoña; Martínez Águila, Alejandro; Pérez de Lara, María Jesús; Miras Portugal, María Teresa; Gómez Villafuertes, María Rosa; Pintor, Jesús
    Glaucoma is a neuropathology, often accompanied by an elevated intraocular pressure (IOP), which can lead to blindness. Since DBA/2J mice develop glaucoma, several studies of the physiopathology of glaucoma have been reported in this animal model. It is also known that purinergic receptors are involved in the pathology of glaucoma by controlling aqueous humor production and drainage and therefore controlling IOP. There are no studies on purinergic receptors in the DBA/2J model of glaucoma and their relation to the development of the pathology, so the aim of this study was to make an approach to the purinergic mechanisms involved in...

  6. Estudio a corto plazo de un tratamiento selvícola en una masa forestal de Quercus illex en el monte de La Hunde (Ayora) sobre propiedades químicas, biológicas y bioquímicas del suel

    FERNÁNDEZ HERNÁNDEZ, GEMMA
    Una manera adecuada de aumentar la calidad de los bosques y por tanto de los suelos es realizar una gestión de la masa forestal encaminada a aumentar la riqueza de especies y la resiliencia del bosque. En este sentido, los tratamientos selvícolas crean huecos en la masa aumentando los niveles de luz en el piso inferior, lo que favorece el desarrollo de la vegetación herbácea y arbustiva. Estos tratamientos suelen conllevar cambios en las condiciones microclimáticas afectando especialmente a la temperatura y humedad del suelo, variables que pueden afectar a su vez a otras propiedades físicas, químicas y biológicas del...

  7. Estudio del efecto a corto plazo de un tratamiento selvícola en una masa forestal de Pinus halepensis en el monte de la Calderona (Serra) sobre propiedades químicas, biológicas y bioquímicas del suelo

    PEÑAS LLOP, MÓNICA
    El cuidado y conservación de los ecosistemas forestales es un tema que, actualmente, resulta de interés mundial, ya que se tratan de la solución a diferentes problemas medioambientales, sociales y culturales. Hacer uso de prácticas de manejo de tierras para asegurar la protección, conservación, restauración y aprovechamiento de los recursos forestales producen alteraciones en las propiedades fisicoquímicas, microbiológicas y bioquímicas del suelo, las cuales pueden llegar a afectar a las funciones del mismo. En este trabajo se han estudiado las propiedades de dos parcelas, una tratada y otra control, para poder comparar las propiedades de cada una y así conocer como varían en un periodo corto de tiempo....

  8. Evolución y seguimiento de pacientes detectados en el nuevo cribado de la Comunidad de Madrid: nuestra experiencia

    Quijada Fraile, María del Pilar
    El cribado neonatal por espectrometría de masas en tandem permite la detección de algunos errores congénitos del metabolismo (ECM) con el fin de iniciar un tratamiento precoz y así poder evitar morbimortalidad y discapacidades asociadas. Así mismo, permite conocer la incidencia real de los ECM que puede variar entre los diferentes países y regiones geográficas. Algunos estudios han demostrado una mejoría en la evolución clínica de algunos ECM. No obstante, algunas cuestiones quedan por aclarar. En la Comunidad de Madrid se inició el cribado ampliado en el año 2011. Objetivos: Conocer la incidencia de los ECM y la evolución clínica...

  9. Polimorfismos en el gen de la calicreína humana 2 (KLK2) y el desarrollo de cáncer de próstata. Relación con la proteína glandular humana 2(HK2) y las formas libre, total y ligada del PSA

    García Barreras, Silvia
    El cáncer de próstata es uno de los tumores más prevalentes en España y uno de los más frecuentemente diagnosticados en el mundo desarrollado. Se trata de un tumor que progresa de manera indetectable y, en muchas ocasiones, las manifestaciones clínicas sólo aparecen cuando la enfermedad es sistémica. Por lo tanto, se trata de un importante problema de salud pública ante el cual se han desarrollado programas de cribado para el diagnóstico precoz. El antígeno prostático específico (PSA) es el marcador tumoral más utilizado para la detección del cáncer de próstata. Sin embargo, la extendida aplicación del PSA como screening...

  10. Analysis of intermediates of steroid transformations in resting cells by thin-layer chromatography (TLC)

    Guevara, Govinda; Perera González, Jesús; Navarro Llorens, Juana-María
    Thin-layer chromatography (TLC) is a useful and convenient method for the analysis of steroids due to: its simple sample preparation, low time-consuming process, high sensitivity, low equipment investment and capacity to work on many samples simultaneously. Here we describe a TLC easy protocol very useful to analyze steroid molecules derived from a biotransformation carried out in wild-type and mutant resting cells of Rhodococcus ruber strain Chol-4. Following this protocol, we were able to detect the presence or the absence of some well-known intermediates of cholesterol catabolism in Rhodococcus, namely AD, ADD, and 9OHAD.

  11. Analysis of intermediates of steroid transformations in resting cells by thin-layer chromatography (TLC)

    Guevara, Govinda; Perera González, Jesús; Navarro Llorens, Juana-María
    Thin-layer chromatography (TLC) is a useful and convenient method for the analysis of steroids due to: its simple sample preparation, low time-consuming process, high sensitivity, low equipment investment and capacity to work on many samples simultaneously. Here we describe a TLC easy protocol very useful to analyze steroid molecules derived from a biotransformation carried out in wild-type and mutant resting cells of Rhodococcus ruber strain Chol-4. Following this protocol, we were able to detect the presence or the absence of some well-known intermediates of cholesterol catabolism in Rhodococcus, namely AD, ADD, and 9OHAD.

  12. Sueños de inmortalidad en el planeta de los genes.

    Fernández-Alonso, M. Carmen; Platón, María
    Cartel anunciador de la charla, (presentación no grabada) dentro del ciclo de charlas “Jam Science-Divulgación Científica” celebradas en el Moe Club (Alberto Alcocer, 32, Madrid) el penúltimo martes de cada mes de 19.30 a 21.00h, desde enero de 2015. Ponente: Carlos López Otín, Catedrático de Bioquímica y Biología Molecular de la Universidad de Oviedo.

  13. Construcción de levaduras capaces de utilizar glucosamina

    Flores, Carmen-Lisset; Gancedo, Carlos
    Póster presentado al XXXIX Congreso anual de la Sociedad Española de Bioquímica y Biología Molecular, celebrado en Salamanca, del 5 al 8 de septiembre 2016.

  14. Functional characterization of 3-ketosteroid 9α-hydroxylases in Rhodococcus ruber strain chol-4

    Guevara, Govinda; Fernández de las Heras, Laura; Perera González, Julián; Navarro LLorens, Juana María
    The 3-Ketosteroid-9α-Hydroxylase, also known as KshAB [androsta-1,4-diene-3,17-dione, NADH:oxygen oxidoreductase (9α-hydroxylating); EC 1.14.13.142)], is a key enzyme in the general scheme of the bacterial steroid catabolism in combination with a 3-ketosteroid-Δ1 -dehydrogenase activity (KstD), being both responsible of the steroid nucleus (rings A/B) breakage. KshAB initiates the opening of the steroid ring by the 9α-hydroxylation of the C9 carbon of 4-ene-3-oxosteroids (e.g. AD) or 1,4-diene-3-oxosteroids (e.g. ADD), transforming them into 9α- hydroxy-4-androsten-3,17-dione (9OHAD) or 9α-hydroxy-1,4-androstadiene-3,17-dione (9OHADD), respectively. The redundancy of these enzymes in the actinobacterial genomes results in a serious difficulty for metabolic engineering this catabolic pathway to obtain intermediates of...

  15. Functional characterization of 3-ketosteroid 9α-hydroxylases in Rhodococcus ruber strain chol-4

    Guevara, Govinda; Fernández de las Heras, Laura; Perera González, Julián; Navarro LLorens, Juana María
    The 3-Ketosteroid-9α-Hydroxylase, also known as KshAB [androsta-1,4-diene-3,17-dione, NADH:oxygen oxidoreductase (9α-hydroxylating); EC 1.14.13.142)], is a key enzyme in the general scheme of the bacterial steroid catabolism in combination with a 3-ketosteroid-Δ1 -dehydrogenase activity (KstD), being both responsible of the steroid nucleus (rings A/B) breakage. KshAB initiates the opening of the steroid ring by the 9α-hydroxylation of the C9 carbon of 4-ene-3-oxosteroids (e.g. AD) or 1,4-diene-3-oxosteroids (e.g. ADD), transforming them into 9α- hydroxy-4-androsten-3,17-dione (9OHAD) or 9α-hydroxy-1,4-androstadiene-3,17-dione (9OHADD), respectively. The redundancy of these enzymes in the actinobacterial genomes results in a serious difficulty for metabolic engineering this catabolic pathway to obtain intermediates of...

  16. Colorectal cancer: from the genetic model to posttranscriptional regulation by noncoding RNAs

    Lizarbe, María Antonia; Calle Espinosa, Jorge; Fernández Lizarbe, Eva; Fernández Lizarbe, Sara; Robles, Miguel Ángel; Olmo López, Nieves; Turnay, Javier
    Colorectal cancer is the third most common form of cancer in developed countries and, despite the improvements achieved in its treatment options, remains as one of the main causes of cancer-related death. In this review, we first focus on colorectal carcinogenesis and on the genetic and epigenetic alterations involved. In addition, noncoding RNAs have been shown to be important regulators of gene expression. We present a general overview of what is known about these molecules and their role and dysregulation in cancer, with a special focus on the biogenesis, characteristics, and function of microRNAs. These molecules are important regulators of...

  17. Cannabinoid Type-2 Receptor Drives Neurogenesis and Improves Functional Outcome After Stroke

    Bravo Ferrer, Isbel; Cuartero Desviat, María Isabel; Zarruk, Juan G.; Pradillo, Jesús M.; Hurtado Moreno, Olivia; Romera, Victor G.; Díaz Alonso, Javier; García Segura, Juan Manuel; Guzmán, Manuel; Lizasoaín, Ignacio; Galve-Roperh, Ismael; Moro, María A.
    Background and Purpose—Stroke is a leading cause of adult disability characterized by physical, cognitive, and emotional disturbances. Unfortunately, pharmacological options are scarce. The cannabinoid type-2 receptor (CB2R) is neuroprotective in acute experimental stroke by anti-inflammatory mechanisms. However, its role in chronic stroke is still unknown. Methods—Stroke was induced by permanent middle cerebral artery occlusion in mice; CB2R modulation was assessed by administering the CB2R agonist JWH133 ((6aR,10aR)-3-(1,1-dimethylbutyl)-6a,7,10,10a-tetrahydro-6,6,9-trimethyl-6Hdibenzo[b,d]pyran) or the CB2R antagonist SR144528 (N-[(1S)-endo-1,3,3-trimethylbicyclo-[2.2.1]-heptan-2-yl]-5-(4- chloro-3-methylphenyl)-1-(4-methylbenzyl)-pyrazole-3-carboxamide) once daily from day 3 to the end of the experiment or by CB2R genetic deletion. Analysis of immunofluorescence-labeled brain sections, 5-bromo-2´-deoxyuridine (BrdU) staining, fluorescence-activated cell...

  18. La ciencia de la sexualidad humana

    Fernández-Alonso, M. Carmen; Platón, María
    Cartel anunciador de la charla; Presentación del ciclo de charlas “Jam Science-Divulgación Científica” celebradas en el Moe Club (Alberto Alcocer, 32, Madrid) el penúltimo martes de cada mes de 19.30 a 21.00h. Ponente: Pere Estupinya. Estudió en la Universitat Rovira i Virgili, donde se especializó en bioquímica; tiene asimismo un máster en nutrición y metabolismo y un doctorado incompleto en genética que abandonó para dedicarse a la divulgación científica. Grabación y edición de los vídeos: DC SciComm.

  19. Structural and lipid-binding characterization of human annexin A13a reveals strong differences with its long A13b isoform

    Fernández Lizarbe, Sara; Lecona, Emilio; Santiago Gómez, Angélica; Olmo, Nieves; Lizarbe, María Antonia; Turnay, Javier
    Annexin A13 is the founder member of the vertebrate family of annexins, which are comprised of a tetrad of unique conserved domains responsible for calcium-dependent binding to membranes. Its expression is restricted to epithelial intestinal and kidney cells. Alternative splicing in the N-terminal region generates two isoforms, A13a and A13b, differing in a deletion of 41 residues in the former. We have confirmed the expression of both isoforms in human colon adenocarcinoma cells at the mRNA and protein levels. We have cloned, expressed, and purified human annexin A13a for the first time to analyze its structural characteristics. Its secondary structure...

  20. Structural and lipid-binding characterization of human annexin A13a reveals strong differences with its long A13b isoform

    Fernández Lizarbe, Sara; Lecona, Emilio; Santiago Gómez, Angélica; Olmo, Nieves; Lizarbe, María Antonia; Turnay, Javier
    Annexin A13 is the founder member of the vertebrate family of annexins, which are comprised of a tetrad of unique conserved domains responsible for calcium-dependent binding to membranes. Its expression is restricted to epithelial intestinal and kidney cells. Alternative splicing in the N-terminal region generates two isoforms, A13a and A13b, differing in a deletion of 41 residues in the former. We have confirmed the expression of both isoforms in human colon adenocarcinoma cells at the mRNA and protein levels. We have cloned, expressed, and purified human annexin A13a for the first time to analyze its structural characteristics. Its secondary structure...

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