Mostrando recursos 1 - 20 de 1.192

  1. Analysis of intermediates of steroid transformations in resting cells by thin-layer chromatography (TLC)

    Guevara, Govinda; Perera González, Jesús; Navarro Llorens, Juana-María
    Thin-layer chromatography (TLC) is a useful and convenient method for the analysis of steroids due to: its simple sample preparation, low time-consuming process, high sensitivity, low equipment investment and capacity to work on many samples simultaneously. Here we describe a TLC easy protocol very useful to analyze steroid molecules derived from a biotransformation carried out in wild-type and mutant resting cells of Rhodococcus ruber strain Chol-4. Following this protocol, we were able to detect the presence or the absence of some well-known intermediates of cholesterol catabolism in Rhodococcus, namely AD, ADD, and 9OHAD.

  2. Sueños de inmortalidad en el planeta de los genes.

    Fernández-Alonso, M. Carmen; Platón, María
    Cartel anunciador de la charla, (presentación no grabada) dentro del ciclo de charlas “Jam Science-Divulgación Científica” celebradas en el Moe Club (Alberto Alcocer, 32, Madrid) el penúltimo martes de cada mes de 19.30 a 21.00h, desde enero de 2015. Ponente: Carlos López Otín, Catedrático de Bioquímica y Biología Molecular de la Universidad de Oviedo.

  3. Construcción de levaduras capaces de utilizar glucosamina

    Flores, Carmen-Lisset; Gancedo, Carlos
    Póster presentado al XXXIX Congreso anual de la Sociedad Española de Bioquímica y Biología Molecular, celebrado en Salamanca, del 5 al 8 de septiembre 2016.

  4. INVESTIGAÇÃO BIOQUÍMICA DAS MUCOPOLISSACARIDOSES: RELATO DA EXPERIÊNCIA DA REDE MPS BRASIL


  5. INVESTIGAÇÃO BIOQUÍMICA DAS MUCOPOLISSACARIDOSES: RELATO DA EXPERIÊNCIA DA REDE MPS BRASIL


  6. REDE MPS BRASIL – ESTIMATIVA DE CUSTOS ASSOCIADOS À INVESTIGAÇÃO BIOQUÍMICA DA MPS IV-A


  7. REDE MPS BRASIL – ESTIMATIVA DE CUSTOS ASSOCIADOS À INVESTIGAÇÃO BIOQUÍMICA DA MPS IV-A


  8. Functional characterization of 3-ketosteroid 9α-hydroxylases in Rhodococcus ruber strain chol-4

    Guevara, Govinda; Fernández de las Heras, Laura; Perera González, Julián; Navarro LLorens, Juana María
    The 3-Ketosteroid-9α-Hydroxylase, also known as KshAB [androsta-1,4-diene-3,17-dione, NADH:oxygen oxidoreductase (9α-hydroxylating); EC 1.14.13.142)], is a key enzyme in the general scheme of the bacterial steroid catabolism in combination with a 3-ketosteroid-Δ1 -dehydrogenase activity (KstD), being both responsible of the steroid nucleus (rings A/B) breakage. KshAB initiates the opening of the steroid ring by the 9α-hydroxylation of the C9 carbon of 4-ene-3-oxosteroids (e.g. AD) or 1,4-diene-3-oxosteroids (e.g. ADD), transforming them into 9α- hydroxy-4-androsten-3,17-dione (9OHAD) or 9α-hydroxy-1,4-androstadiene-3,17-dione (9OHADD), respectively. The redundancy of these enzymes in the actinobacterial genomes results in a serious difficulty for metabolic engineering this catabolic pathway to obtain intermediates of...

  9. Functional characterization of 3-ketosteroid 9α-hydroxylases in Rhodococcus ruber strain chol-4

    Guevara, Govinda; Fernández de las Heras, Laura; Perera González, Julián; Navarro LLorens, Juana María
    The 3-Ketosteroid-9α-Hydroxylase, also known as KshAB [androsta-1,4-diene-3,17-dione, NADH:oxygen oxidoreductase (9α-hydroxylating); EC 1.14.13.142)], is a key enzyme in the general scheme of the bacterial steroid catabolism in combination with a 3-ketosteroid-Δ1 -dehydrogenase activity (KstD), being both responsible of the steroid nucleus (rings A/B) breakage. KshAB initiates the opening of the steroid ring by the 9α-hydroxylation of the C9 carbon of 4-ene-3-oxosteroids (e.g. AD) or 1,4-diene-3-oxosteroids (e.g. ADD), transforming them into 9α- hydroxy-4-androsten-3,17-dione (9OHAD) or 9α-hydroxy-1,4-androstadiene-3,17-dione (9OHADD), respectively. The redundancy of these enzymes in the actinobacterial genomes results in a serious difficulty for metabolic engineering this catabolic pathway to obtain intermediates of...

  10. Colorectal cancer: from the genetic model to posttranscriptional regulation by noncoding RNAs

    Lizarbe, María Antonia; Calle Espinosa, Jorge; Fernández Lizarbe, Eva; Fernández Lizarbe, Sara; Robles, Miguel Ángel; Olmo López, Nieves; Turnay, Javier
    Colorectal cancer is the third most common form of cancer in developed countries and, despite the improvements achieved in its treatment options, remains as one of the main causes of cancer-related death. In this review, we first focus on colorectal carcinogenesis and on the genetic and epigenetic alterations involved. In addition, noncoding RNAs have been shown to be important regulators of gene expression. We present a general overview of what is known about these molecules and their role and dysregulation in cancer, with a special focus on the biogenesis, characteristics, and function of microRNAs. These molecules are important regulators of...

  11. Cannabinoid Type-2 Receptor Drives Neurogenesis and Improves Functional Outcome After Stroke

    Bravo Ferrer, Isbel; Cuartero Desviat, María Isabel; Zarruk, Juan G.; Pradillo, Jesús M.; Hurtado Moreno, Olivia; Romera, Victor G.; Díaz Alonso, Javier; García Segura, Juan Manuel; Guzmán, Manuel; Lizasoaín, Ignacio; Galve-Roperh, Ismael; Moro, María A.
    Background and Purpose—Stroke is a leading cause of adult disability characterized by physical, cognitive, and emotional disturbances. Unfortunately, pharmacological options are scarce. The cannabinoid type-2 receptor (CB2R) is neuroprotective in acute experimental stroke by anti-inflammatory mechanisms. However, its role in chronic stroke is still unknown. Methods—Stroke was induced by permanent middle cerebral artery occlusion in mice; CB2R modulation was assessed by administering the CB2R agonist JWH133 ((6aR,10aR)-3-(1,1-dimethylbutyl)-6a,7,10,10a-tetrahydro-6,6,9-trimethyl-6Hdibenzo[b,d]pyran) or the CB2R antagonist SR144528 (N-[(1S)-endo-1,3,3-trimethylbicyclo-[2.2.1]-heptan-2-yl]-5-(4- chloro-3-methylphenyl)-1-(4-methylbenzyl)-pyrazole-3-carboxamide) once daily from day 3 to the end of the experiment or by CB2R genetic deletion. Analysis of immunofluorescence-labeled brain sections, 5-bromo-2´-deoxyuridine (BrdU) staining, fluorescence-activated cell...

  12. La ciencia de la sexualidad humana

    Fernández-Alonso, M. Carmen; Platón, María
    Cartel anunciador de la charla; Presentación del ciclo de charlas “Jam Science-Divulgación Científica” celebradas en el Moe Club (Alberto Alcocer, 32, Madrid) el penúltimo martes de cada mes de 19.30 a 21.00h. Ponente: Pere Estupinya. Estudió en la Universitat Rovira i Virgili, donde se especializó en bioquímica; tiene asimismo un máster en nutrición y metabolismo y un doctorado incompleto en genética que abandonó para dedicarse a la divulgación científica. Grabación y edición de los vídeos: DC SciComm.

  13. Structural and lipid-binding characterization of human annexin A13a reveals strong differences with its long A13b isoform

    Fernández Lizarbe, Sara; Lecona, Emilio; Santiago Gómez, Angélica; Olmo, Nieves; Lizarbe, María Antonia; Turnay, Javier
    Annexin A13 is the founder member of the vertebrate family of annexins, which are comprised of a tetrad of unique conserved domains responsible for calcium-dependent binding to membranes. Its expression is restricted to epithelial intestinal and kidney cells. Alternative splicing in the N-terminal region generates two isoforms, A13a and A13b, differing in a deletion of 41 residues in the former. We have confirmed the expression of both isoforms in human colon adenocarcinoma cells at the mRNA and protein levels. We have cloned, expressed, and purified human annexin A13a for the first time to analyze its structural characteristics. Its secondary structure...

  14. Structural and lipid-binding characterization of human annexin A13a reveals strong differences with its long A13b isoform

    Fernández Lizarbe, Sara; Lecona, Emilio; Santiago Gómez, Angélica; Olmo, Nieves; Lizarbe, María Antonia; Turnay, Javier
    Annexin A13 is the founder member of the vertebrate family of annexins, which are comprised of a tetrad of unique conserved domains responsible for calcium-dependent binding to membranes. Its expression is restricted to epithelial intestinal and kidney cells. Alternative splicing in the N-terminal region generates two isoforms, A13a and A13b, differing in a deletion of 41 residues in the former. We have confirmed the expression of both isoforms in human colon adenocarcinoma cells at the mRNA and protein levels. We have cloned, expressed, and purified human annexin A13a for the first time to analyze its structural characteristics. Its secondary structure...

  15. Capacidade funcional e adesão ao regime terapêutico: a realidade de uma população idosa

    Marcos, Alípio Augusto; Magalhães, Carlos Pires; Fernandes, Adília; Mata, Maria Augusta
    Em Portugal, nas últimas décadas, o aumento da esperança média de vida, o aumento do índice de envelhecimento, bem como do índice de longevidade, são uma realidade. Tendo por base os últimos censos realizados pelo Instituto Nacional de Estatística (lNE), entre 2001 e 2011, o índice de envelhecimento aumentou de 102,23 pessoas idosas por cada 100 jovens, para 127,8 (1). O concelho de Macedo de Cavaleiros constitui um dos concelhos com um envelhecimento demográfico exacerbado, pois em 2011 o índice de envelhecimento alcançou os 240 (2), acarretando preocupações, mas também desafios. o envelhecimento é um processo dinâmico e progressivo, responsável por alterações morfológicas, bioquímicas e psicológicas. Estas...

  16. Capacidade funcional e adesão ao regime terapêutico: a realidade de uma população idosa

    Marcos, Alípio Augusto; Magalhães, Carlos Pires; Fernandes, Adília; Mata, Maria Augusta
    Em Portugal, nas últimas décadas, o aumento da esperança média de vida, o aumento do índice de envelhecimento, bem como do índice de longevidade, são uma realidade. Tendo por base os últimos censos realizados pelo Instituto Nacional de Estatística (lNE), entre 2001 e 2011, o índice de envelhecimento aumentou de 102,23 pessoas idosas por cada 100 jovens, para 127,8 (1). O concelho de Macedo de Cavaleiros constitui um dos concelhos com um envelhecimento demográfico exacerbado, pois em 2011 o índice de envelhecimento alcançou os 240 (2), acarretando preocupações, mas também desafios. o envelhecimento é um processo dinâmico e progressivo, responsável por alterações morfológicas, bioquímicas e psicológicas. Estas...

  17. Pulmonary surfactant and nanocarriers: toxicity versus combined nanomedical applications

    Hidalgo Román, Alberto; Cruz, Antonio; Pérez Gil, Jesús
    Pulmonary surfactant is a membrane-based lipid-protein system essential for the process of breathing, which coats and stabilizes the whole respiratory surface and possesses exceptional biophysical properties. It constitutes the first barrier against the entry of pathogens and harmful particles in the alveolar region, extended through the lungs, but on the other hand, it can offer novel possibilities as a shuttle for the delivery of drugs and nanocarriers. The advances in nanotechnology are opening the doors to new diagnostic and therapeutic avenues, which are not accessible by means of the current approaches. In this context, the pulmonary route is called to...

  18. Análise toxicológica de suplementos alimentares e compostos emagrecedores contendo efedrina, p-sinefrina e cafeína

    Fagundes, Ana Cláudia
    A busca por um padrão estético globalizado e o aumento da obesidade fazem crescer o uso de suplementos alimentares e compostos emagrecedores à base de extratos vegetais. Produtos contendo a associação de p-sinefrina, efedrina, salicina e cafeína são amplamente consumidos e não apresentam efetividade e segurança bem esclarecidas. Portanto, o objetivo deste trabalho foi avaliar a toxicidade subcrônica de p-sinefrina, efedrina, cafeína e salicina, isoladas e em associação, em ratos Wistar machos. Doses de salicina 6 mg/kg, efedrina 4 mg/kg, p-sinefrina 10 mg/kg, cafeína 80 mg/kg e a associação de salicina, efedrina, p-sinefrina e cafeína 100 mg/kg (6:4:10:80, respectivamente) foram...

  19. Pulmonary surfactant metabolism in the alveolar airspace: Biogenesis,extracellular conversions, recycling

    Olmeda Lozano, Bárbara; Martínez Calle, Marta; Pérez Gil, Jesús
    Pulmonary surfactant is a lipid–protein complex that lines and stabilizes the respiratory interface in the alveoli, allowing for gas exchange during the breathing cycle. At the same time, surfactant constitutes the first line of lung defense against pathogens. This review presents an updated view on the processes involved in biogenesis and intracellular processing of newly synthesized and recycled surfactant components, as well as on the extracellular surfactant transformations before and after the formation ofthe surface active film atthe air–water interface. Special attention is paid to the crucial regulation of surfactant homeostasis, because its disruption is associated with several lung pathologies.

  20. La enzima amidohidrolasa de ácidos grasos como diana terapéutica en modelos de neuroinflamación aguda y crónica

    Vázquez Fernández, Carmen
    La enfermedad de Alzheimer (EA) es un trastorno neurodegenerativo asociado a la edad, en el cual hay una alteración de múltiples funciones incluyendo la memoria, el pensamiento, el juicio y el comportamiento. A excepción de algunos casos de Alzheimer causados por mutaciones genéticas, los expertos creen que la enfermedad se desarrolla debido al resultado de múltiples factores y no a una única causa, aunque la edad es el principal factor de riesgo. El curso de la enfermedad está muy relacionado con los cambios neuropatológicos que ocurren en el cerebro de estos pacientes. Estos cambios se caracterizan fundamentalmente por el deterioro...

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