Mostrando recursos 1 - 20 de 30.169

  1. Dinâmica de infecção de Mycoplasma hyopneumoniae em leitoas de reposição

    Takeuti, Karine Ludwig
    A infecção por Mycoplasma (M.) hyopneumoniae é responsável por perdas econômicas significativas na suinocultura, causando uma pneumonia crônica que normalmente afeta clinicamente suínos de crescimento e terminação. Considerando-se a importância das matrizes de menor ordem de parto na transmissão de M. hyopneumoniae e que uma grande quantidade de leitoas é introduzida nos planteis anualmente, este projeto teve como objetivo compreender aspectos pouco conhecidos da dinâmica de infecção de M. hyopneumoniae em leitoas de reposição. O primeiro estudo avaliou a dinâmica e persistência da infecção por M. hyopneumoniae em leitoas em condições naturais de campo. Quarenta e quatro leitoas foram selecionadas...

  2. Resectable pancreatic head adenocarcinoma: Is R0 resection an illusion? Genetic evaluation of venous resection margin affirmed unrecognized disease

    Turrini , O.; Gilabert , M.; Ewald , J.; Moutardier , V.; Delpero , J.-R.; Iovanna , J.-L.; Turrini , M
    International audience

  3. Genetic characteristics of toxigenic Clostridia and toxin gene evolution

    Popoff , Michel ,; Bouvet , Philippe
    International audience

  4. One-gene-one-enzyme: Remembering biochemical genetics

    Horowitz, Norman H.
    The discovery that proteins are encoded in the genes, which are themselves not proteins, has long seemed to me to be the key to understanding the organization of living matter. To be able to state with confidence that the genetic part of the organism - the part that is transmitted from generation to generation - consists of instructions in the form of DNA for the synthesis of proteins, which later produce and operate the organism, implies a depth of knowledge that would have astonished biologists of an earlier day. In their Enzymes (3rd edition, 1979), Dixon and Webb called this...

  5. Fertility testing and ICSI sperm selection by hyaluronic acid binding: clinical and genetic aspects

    Huszár, Gábor; Jakab, Attila; Sakkas, Denny; Ozenci, Ciler-Celik; Cayli, Sevil; Delpiano, Elena; Ozkavukcu, Sinan

  6. Comparison of transcriptome-derived simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers for genetic fingerprinting, diversity evaluation, and establishment of relationships in eggplants

    Gramazio, Pietro; Prohens Tomás, Jaime; Borras, Dionís; Plazas Ávila, María de la O; Herraiz García, Francisco Javier; Vilanova Navarro, Santiago
    [EN] Simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers are amongst the most common markers of choice for studies of diversity and relationships in horticultural species. We have used 11 SSR and 35 SNP markers derived from transcriptome sequencing projects to fingerprint 48 accessions of a collection of brinjal (Solanum melongena), gboma (S. macrocarpon) and scarlet (S. aethiopicum) eggplant complexes, which also include their respective wild relatives S. incanum, S. dasyphyllum and S. anguivi. All SSR and SNP markers were polymorphic and 34 and 36 different genetic fingerprints were obtained with SSRs and SNPs, respectively. When combining both...

  7. Introgressiomics: a new approach for using crop wild relatives in breeding for adaptation to climate change

    Prohens Tomás, Jaime; Gramazio, Pietro; Plazas Ávila, María de la O; Dempewolf, Hannes; Kilian, Benjamin; Díez-Niclós, María José Teresa de Jesús; Fita, Ana; Herraiz García, Francisco Javier; Rodríguez Burruezo, Adrián; Soler Aleixandre, Salvador; Knapp, Sandra; Vilanova Navarro, Santiago
    [EN] The need to boost agricultural production in the coming decades in a climate change scenario requires new approaches for the development of new crop varieties that are more resilient and more efficient in the use of resources. Crop wild relatives (CWRs) are a source of variation for many traits of interest in breeding, in particular tolerance to abiotic and biotic stresses. However, their potential in plant breeding has largely remained unexploited. CWRs can make an effective contribution to broadening the genetic base of crops and to introgressing traits of interest, but their direct use by breeders in breeding programs...

  8. Loss of UBE2O Mitigates Beta Thalassemia Through Broad Proteomic Changes

    Nguyen, Anthony Tuan
    UBE2O is a ubiquitin-conjugating enzyme that is upregulated during terminal erythroid differentiation. We previously showed that UBE2O selectively ubiquitinates ribosomal proteins (RPs) and drives the elimination of ribosomes in reticulocytes and non-erythroid cells. Here, we showed that Ube2o-/- reticulocytes have a severe defect in the elimination of RPs by immunoblot analysis. We then used quantitative mass spectrometry to analyze the reticulocyte proteome in a global and unbiased manner. Of the 1235 proteins quantified, we observed significant elevations of 183 proteins, many of which were RPs. Similarly, we quantified the proteomic changes in HEK293-derived cells upon expression of UBE2O. Nearly 10%...

  9. Investigating the Molecular Mechanisms of Action of Lenalidomide and Other Immunomodulatory Derivatives

    Haldar, Saurav Daniel
    Background: Thalidomide was first released as a sedative and anti-emetic by a German pharmaceutical company in the 1950s. After its withdrawal from the market due to severe teratogenicity, thalidomide and its novel iMiD analogs were repurposed decades later for a variety of new clinical indications, including the treatment of erythema nodosum leprosum, multiple myeloma, and del(5q)-MDS. Recent work has demonstrated that iMiDs act by modulating the substrate specificity of the CRBN-CRL4 E3 ubiquitin ligase. Selective degradation of IKZF1/IKZF3 and CK1α by this complex in the presence of iMiDs leads to therapeutic activity against multiple myeloma and del(5q)-MDS, respectively. In spite...

  10. The Effect of Human Genetic Variation on CRISPR Targeting Specificity

    Canver, Matthew C.
    The clustered regularly interspaced short palindromic repeats (CRISPR) nuclease system offers the ability for unprecedented functional genetic experiments and the promise for therapy of a variety of genetic disorders. The understanding of factors contributing to CRISPR targeting efficacy and specificity continue to evolve. As CRISPR systems rely on Watson-Crick base pairing to ultimately mediate genomic cleavage, it logically follows that genetic variation would affect CRISPR targeting by increasing or decreasing sequence homology at on- and off-target sites or by altering protospacer adjacent motifs (PAMs). Numerous efforts have been made to document the extent of human genetic variation, which serve as...

  11. Synthetic Lethal Interactions With Oncogenic KRAS

    Wang, Belinda
    KRAS is one of the most frequently mutated genes across human cancers, including 96% of pancreatic cancers, 40% of colorectal cancers, and 35% of lung cancers. The majority of human cancer cell lines and tumors from genetically engineered mouse models harboring an oncogenic mutant KRAS allele demonstrate a strong dependence on KRAS for proliferation and survival. This KRAS dependency is a type of ‘oncogene addiction,’ a state in which cancer cells depend on signaling from a single oncogene for survival. Unfortunately, the development of clinically effective KRAS-directed cancer therapies has been unsuccessful, and KRAS-mutant cancers are refractory to standard and...

  12. The impact of serotype-specific vaccination on phylodynamic parameters of Streptococcus pneumoniae and the pneumococcal pan-genome

    Azarian, Taj; Grant, Lindsay R.; Arnold, Brian John; Hammitt, Laura L.; Reid, Raymond; Santosham, Mathuram; Weatherholtz, Robert; Goklish, Novalene; Thompson, Claudette M.; Bentley, Stephen D.; O’Brien, Katherine L.; Hanage, William P.; Lipsitch, Marc
    In the United States, the introduction of the heptavalent pneumococcal conjugate vaccine (PCV) largely eliminated vaccine serotypes (VT); non-vaccine serotypes (NVT) subsequently increased in carriage and disease. Vaccination also disrupts the composition of the pneumococcal pangenome, which includes mobile genetic elements and polymorphic non-capsular antigens important for virulence, transmission, and pneumococcal ecology. Antigenic proteins are of interest for future vaccines; yet, little is known about how the they are affected by PCV use. To investigate the evolutionary impact of vaccination, we assessed recombination, evolution, and pathogen demographic history of 937 pneumococci collected from 1998-2012 among Navajo and White Mountain Apache...

  13. Crescimento de frangos de corte de diferentes potenciais genéticos consumindo dietas com níveis crescentes de metionina + cisteína A em dois níveis de proteína bruta

    Goldenberg, Daniel Brum
    Neste experimento foi avaliado a resposta de frangos machos da linhagem Cobb x Cobb 500 e Ross x Ross 308, alimentados com dois níveis de proteína bruta e relações graduais de metionina + cisteína, dos 14 aos 35 dias de idade. A dieta foi formulada a base de milho, farelo de soja e farinha de carne, com 3060 kcal/kg e 26 % de proteína bruta, contendo 1,46 % de lisina digestível verdadeira. A concentração de metionina + cisteína digestível (58:42%) foi 0,73 % (50 %em relação à lisina), os outros nutrientes seguiram as recomendações do NRC (1994). Nesta ração foi...

  14. Resistência a antimicrobianos e fatores de virulência em Klebsiella sp. isoladas da laguna de Tramandaí

    Nunes, Athos Aramis Tópor
    A resistência antimicrobiana e fatores de virulência são importantes mecanismos de sobrevivência das bactérias, através deles elas se tornaram capazes de escapar da ação das adversidades do ambiente. O presente trabalho teve como objetivo principal analisar os fatores de virulência e genes de resistência de isolados de Klebsiella sp. e sua participação na manutenção de populações bacterianas resistentes em pontos na Laguna de Tramandaí. As amostragens foram realizadas em agosto de 2014 e janeiro de 2015 em quatro pontos distintos com diferentes graus de impacto ambiental. Foram analisados 272 isolados bacterianos das amostras de água da laguna de Tramandaí. Estes...

  15. Identification of genetic determinants of breast cancer immune phenotypes by integrative genome-scale analysis

    Hendrickx , Wouter; Simeone , Ines; Anjum , Samreen; Mokrab , Younes; Bertucci , François; Finetti , Pascal; Curigliano , Giuseppe; Seliger , Barbara; Cerulo , Luigi; Tomei , Sara; Delogu , Lucia ,; Maccalli , Cristina; Wang , Ena; Miller , Lance ,; Marincola , Francesco; Ceccarelli , Michele; Bedognetti , Davide
    International audience

  16. Pheochromocytoma: A genetic and diagnostic update

    Mercado-Asis , Leilani ,; Wolf , Katherine ,; Jochmanova , Ivana; Taïeb , David
    International audience

  17. Pathological and Genetic Characterization of Bilateral Adrenomedullary Hyperplasia in a Patient with Germline MAX Mutation

    Romanet , Pauline; Guerin , Carole; Pedini , Pascal; Essamet , Wassim; Castinetti , Frederic; Sebag , Frédéric; Roche , Philippe; Cascon , Alberto; Tischler , Arthur; Pacak , Karel; Barlier , Anne ,; Taieb , David
    International audience

  18. CASTLE tumor of the parotid: First documented case, literature review, and genetic analysis of the cancer

    Wong , Eugene Hung Chih; Tetter , Nora; Tzankov , Alexandar; Muller , Laurent
    International audience

  19. Adipogenic genes expression in relation to hepatic steatosis in the liver of two duck species

    Herault , Frédéric; Duby , Cécile; Baeza , Elisabeth; Diot , Christian
    Some studies have shown that expression of peroxisome proliferator-activated receptor gamma (PPARG), a key regulator of adipogenesis, and of some adipocyte-specific genes or adipokines are expressed in hepatic steatosis, leading to the concept of 'adipogenic hepatic steatosis' or 'hepatic adiposis.' Most of these studies were conducted in genetic obese mouse models or after manipulation of gene expression. The relevance of this concept to other species and more physiological models was here addressed in ducks which are able to develop hepatic steatosis after overfeeding. The expression of PPARG and other adipocyte-specific genes was thus analyzed in the liver of ducks fed...

  20. Ocean current connectivity propelling the secondary spread of a marine invasive comb jelly across western Eurasia

    Jaspers, Cornelia; Huwer, Bastian; Antajan, Elvire; Hosia, Aino; Hinrichsen, Hans-harald; Biastoch, Arne; Angel, Dror; Asmus, Ragnhild; Augustin, Christina; Bagheri, Siamak; Beggs, Steven E.; Balsby, Thorsten J. S.; Boersma, Maarten; Bonnet, Delphine; Christensen, Jens T.; Dänhardt, Andreas; Delpy, Floriane; Falkenhaug, Tone; Finenko, Galina; Fleming, Nicholas E. C.; Fuentes, Veronica; Galil, Bella; Gittenberger, Arjan; Griffin, Donal C.; Haslob, Holger; Javidpour, Jamileh; Kamburska, Lyudmila; Kube, Sandra; Langenberg, Victor T.; Lehtiniemi, Maiju; Lombard, Fabien; Malzahn, Arne; Marambio, Macarena; Mihneva, Veselina; Møller, Lene Friis; Niermann, Ulrich; Okyar, Melek Isinibilir; Özdemir, Zekiye Birinci; Pitois, Sophie; Reusch, Thorsten B. H.; Robbens, Johan; Stefanova, Kremena; Thibault, Delphine; Van Der Veer, Henk W.; Vansteenbrugge, Lies; Van Walraven, Lodewijk; Woźniczka, Adam
    Aim Invasive species are of increasing global concern. Nevertheless, the mechanisms driving further distribution after the initial establishment of non‐native species remain largely unresolved, especially in marine systems. Ocean currents can be a major driver governing range occupancy, but this has not been accounted for in most invasion ecology studies so far. We investigate how well initial establishment areas are interconnected to later occupancy regions to test for the potential role of ocean currents driving secondary spread dynamics in order to infer invasion corridors and the source–sink dynamics of a non‐native holoplanktonic biological probe species on a continental scale. Location Western Eurasia. Time period 1980s–2016. Major...

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