FREEDOM D3.2 Interference Coordination Protocols in Femto-based Networks - Agustín de Dios, Adrián; Muñoz Medina, Olga; Vidal Manzano, José; Goldhammer, Mariana; Rodríguez Fonollosa, Javier; Pastore, Adriano; Sardellitti, Stefania; Pescosolido, Loreto; di Lorenzo, Paolo; Barbarossa, Sergio; Baiocchi, Andrea; de Marinis, Enrico; Imponente, Giovanni; Pucci, Fabrizio; Rosi, Guido
This document looks into coordinated radio resource allocation algorithms in deployments of huge
number of femto-access points (FAP) by exploiting the message exchange at control-plane level through
the wired ISP backhaul link. The algorithms present in this document are based on decentralized
cooperative strategies supported by Game Theory. Decentralized resource allocation algorithms based on
price exchange have been derived under different criteria: guaranteeing a minimum rate with the
minimum power, maximizing the weighted sum-rate of the system, maximizing the opportunistic
throughput when there are different source of randomness like random link failures and noise
quantization or when the activity of macro-users is not known by FAPs (coordinated...
Hyperhomocysteinemia causes premature hearing loss in C57BL/6J mice - Martínez-Vega, Raquel; Varela, Gregorio; Partearroyo, Teresa; Pajares, María A.; Varela-Nieto, Isabel
[Introduction]: Alterations in plasma homocysteine (pHcy) levels and betaine homocysteine methyltransferase (BHMT) expression have been reported in genetic mouse models of human deafness. BHMT is one of the enzymes responsible for Hcy remethylation leading to methionine synthesis, its changes being one of the causes of increased pHcy levels and indirectly altering production of glutathione (2). Nutritional factors modulate Hcy metabolism, and hence we have studied the impact of a dietary-induced folic acid deficiency on the cochlear methionine metabolism and in hearing. [Material and Methods]: Hearing capacity from the control and folate deficient diet groups was assessed by ABR threshold analyses...
Control óptimo en canales de riego: estudio de casos test ASCE - Ambroj Pérez, Samuel; García-Navarro, Pilar; Burguete Tolosa, Javier
En este trabajo se han estudiado varios casos propuestos por ASCE sobre algoritmos de automatización de canales. Estos se basan en dos canales operativos en la actualidad, siendo el comportamiento de ellos muy diferente. El canal 1 presenta tiempos de respuesta rápidos y tiene poca capacidad de almacenamiento; mientras que, el canal 2 es tres veces más extenso, menos empinado y tiene una capacidad de almacenamiento superior. Ambos canales tienen 8 tramos separados por compuertas transversales verticales. El objetivo de estos tests es controlar el nivel de agua en 8 puntos de control situados a Sm del final de cada...
Insights to controls on dolomitization by means of reactive transport models applied to the Benicàssim case study (Maestrat Basin, eastern Spain) - Corbella, M.; Gomez-Rivas, E.; Martín-Martín, Juan Diego; Stafford, S.L.; Teixell, A.; Griera, A.; Travé, A.; Cardellach, E.; Salas, R.
Partially dolomitized carbonate rocks of the Middle East and North America host large hydrocarbon reserves. The origin of some of these dolomites has been attributed to a hydrothermal mechanism. The Benicàssim area (Maestrat Basin, eastern Spain) constitutes an excellent field analogue for fault-controlled stratabound hydrothermal dolomitization: dolostone geobodies are well exposed and extend over several kilometres away from seismic-scale faults. This work investigates the main controls on the formation of stratabound versus massive dolomitization in carbonate sequences by means of two-dimensional (2D) reactive transport models applied to the Benicàssim case study. Simulation results suggest that the dolomitization capacity of Mg-rich...
Los bloques de hielo que caen del cielo : Antecedentes y fenomenología reciente - Martínez-Frías, J.; López Vera, F.
[EN]: Recently, blocks of ice have been falling from Spain’s clear skies. These lumps of ice were sizeable,
weighing up to 3 kg. Although the falls are very unusual, many other previous cases have been described,
and some references have been published in the scientific journals. This paper summarises the “state-ofthe-
art”, and describes other outstanding similar falls, which are coincidental with those occurred in
Spain. Finally, the main genetic probable scenarios are indicated.
A hirtelen szívhalál molekuláris és genetikai háttere - Simkó, József; Szabó, Zoltán; Barta, Kitti; Újvárosi, Dóra; Nánási, Péter; Lőrincz, István; Szabó Zoltán (1973-) (belgyógyász, kardiológus); Lörincz István (1950-) (belgyógyász, kardiológus); Balla József (1959-) (belgyógyász, nephrológus); Lörincz István (1950-) (belgyógyász, kardiológus); Kun Csaba (1969-) (kardiológus); Padra János Tamás (1984-) (biológus)
Despite recent findings on the functional, structural and genetic background of sudden cardiac death, the incidence is still relatively high in the entire population. A thorough knowledge on susceptibility, as well as pathophysiology behind the development of malignant arrhythmias will help us to identify individuals at risk and prevent sudden cardiac death. This article presents a review of the current literature on the role of altered intracellular Ca2+ handling, acute myocardial ischaemia, cardiac autonomic innervation, renin-angiotensin-aldosterone system, monogenic and complex heritability in the pathogenesis of sudden cardiac death
Ajuba Family Proteins Link JNK to Hippo Signaling - Sun, Gongping; Irvine, Kenneth D.
Wounding, apoptosis, or infection can trigger a proliferative response in neighboring cells to replace damaged tissue. Studies in Drosophila have implicated Jun N-terminal kinase (JNK)-dependent activation of Yorkie (Yki) as essential to regeneration-associated growth, as well as growth associated with neoplastic tumors. Yki is a transcriptional co-activator that is inhibited by Hippo signaling, a conserved pathway that regulates growth. We found identified a conserved mechanism by which JNK regulated Hippo signaling. Genetic studies in Drosophila identified Jub (also known as Ajuba LIM protein) as required for JNK-mediated activation of Yki, and showed that Jub contributed to wing regeneration after wounding...
Comprehensive experimental fitness landscape and evolutionary network for small RNA - Jiménez, José I.; Xulvi-Brunet, Ramon; Campbell, Gregory W.; Turk-MacLeod, Rebecca; Chen, Irene A.
Evolution by natural selection is driven by fitness differences, which define a “fitness landscape” in the space of all possible genetic sequences. Understanding the landscape is critical for understanding and predicting natural selection, yet very little is known about the structure of a real fitness landscape. Here we experimentally determine the complete fitness landscape of small RNA selected to interact with GTP, a building block for early life. We find that the landscape is composed of largely disconnected islands of active sequences. This scenario suggests that natural selection under these conditions would be constrained to local exploration of sequence space...
Discovery of the cellular and molecular basis of cholesterol control - Schekman, Randy
The cellular control of cholesterol metabolism mediated by lipoproteins was first appreciated in pioneering work published in a 1974 PNAS Classic by Michael Brown and Joseph Goldstein. We know from this paper that the LDL binds to a cell surface receptor and dampens the activity of a key enzyme in cholesterol biosynthesis and that a receptor deficiency is responsible for a major genetic cause of hypercholesterolemia and premature atherosclerosis.
Cyclin D3 coordinates the cell cycle during differentiation to regulate erythrocyte size and number - Eng, Jennifer Christina; Lander, Eric S.; Lodish, Harvey F.; Sankaran, Vijay G.; Ludwig, Leif S.; Sicinska, Ewa; Xu, Jian; Bauer, Daniel E.; Patterson, Heide Christine; Metcalf, Ryan A.; Natkunam, Yasodha; Orkin, Stuart H.; Sicinski, Piotr
Genome-wide association studies (GWASs) have identified a genetic variant of moderate effect size at 6p21.1 associated with erythrocyte traits in humans. We show that this variant affects an erythroid-specific enhancer of CCND3. A Ccnd3 knockout mouse phenocopies these erythroid phenotypes, with a dramatic increase in erythrocyte size and a concomitant decrease in erythrocyte number. By examining human and mouse primary erythroid cells, we demonstrate that the CCND3 gene product cyclin D3 regulates the number of cell divisions that erythroid precursors undergo during terminal differentiation, thereby controlling erythrocyte size and number. We illustrate how cell type-specific specialization can occur for general...
Bacteria and the Aging and Longevity of Caenorhabditis elegans - Kim, Dennis H.
The molecular genetic analysis of longevity of Caenorhabditis elegans has yielded fundamental insights into evolutionarily conserved pathways and processes governing the physiology of aging. Recent studies suggest that interactions between C. elegans and its microbial environment may influence the aging and longevity of this simple host organism. Experimental evidence supports a role for bacteria in affecting longevity through distinct mechanisms—as a nutrient source, as a potential pathogen that induces double-edged innate immune and stress responses, and as a coevolved sensory stimulus that modulates neuronal signaling pathways regulating longevity. Motivating this review is the anticipation that the molecular genetic dissection of...
Production of unnaturally linked chimeric proteins using a combination of sortase-catalyzed transpeptidation and click chemistry - Witte, Martin D; Theile, Christopher S; Wu, Tongfei; Guimaraes, Carla P; Blom, Annet E M; Ploegh, Hidde
Chimeric proteins, including bispecific antibodies, are biological tools with therapeutic applications. Genetic fusion and ligation methods allow the creation of N-to-C and C-to-N fused recombinant proteins, but not unnaturally linked N-to-N and C-to-C fusion proteins. This protocol describes a simple procedure for the production of such chimeric proteins, starting from correctly folded proteins and readily available peptides. By equipping the N terminus or C terminus of the proteins of interest with a set of click handles using sortase A, followed by a strain-promoted click reaction, unnatural N-to-N and C-to-C linked (hetero) fusion proteins are established. Examples of proteins that have...
Gentamicin, genetic variation and deafness in preterm children. - Bitner-Glindzicz, M; Rahman, S; Chant, K; Marlow, N
Hearing loss in children born before 32 weeks of gestation is more prevalent than in full term infants. Aminoglycoside antibiotics are routinely used to treat bacterial infections in babies on neonatal intensive care units. However, this type of medication can have harmful effects on the auditory system. In order to avoid this blood levels should be maintained in the therapeutic range. However in individuals with a mitochondrial genetic variant (m.1555A > G), permanent hearing loss can occur even when drug levels are within normal limits. The aim of the study is to investigate the burden that the m.1555A > G...
Deciphering molecular circuits from genetic variation underlying transcriptional responsiveness to stimuli - Gat-Viks, Irit; Chevrier, Nicolas; Wilentzik, Roni; Eisenhaure, Thomas; Raychowdhury, Raktima; Steuerman, Yael; Shalek, Alex; Hacohen, Nir; Amit, Ido; Regev, Aviv
Individual genetic variation affects gene expression in response to stimuli, often by influencing complex molecular circuits. Here we combine genomic and intermediate-scale transcriptional profiling with computational methods to identify variants that affect the responsiveness of genes to stimuli (responsiveness QTLs; reQTLs) and to position these variants in molecular circuit diagrams. We apply this approach to study variation in transcriptional responsiveness to pathogen components in dendritic cells from recombinant inbred mouse strains. We identify reQTLs that correlate with particular stimuli and position them in known pathways. For example, in response to a virus-like stimulus, a trans-acting variant acts as an activator...
Targeted Deletion and Inversion of Tandemly Arrayed Genes in Arabidopsis thaliana Using Zinc Finger Nucleases - Qi, Yiping; Li, Xiaohong; Zhang, Yong; Starker, Colby G.; Baltes, Nicholas J.; Zhang, Feng; Sander, Jeffry D.; Reyon, Deepak; Joung, J. Keith; Voytas, Daniel F.
Tandemly arrayed genes (TAGs) or gene clusters are prevalent in higher eukaryotic genomes. For example, approximately 17% of genes are organized in tandem in the model plant Arabidopsis thaliana. The genetic redundancy created by TAGs presents a challenge for reverse genetics. As molecular scissors, engineered zinc finger nucleases (ZFNs) make DNA double-strand breaks in a sequence-specific manner. ZFNs thus provide a means to delete TAGs by creating two double-strand breaks in the gene cluster. Using engineered ZFNs, we successfully targeted seven genes from three TAGs on two Arabidopsis chromosomes, including the well-known RPP4 gene cluster, which contains eight resistance (R)...
HLA Class I and Class II Conserved Extended Haplotypes and Their Fragments or Blocks in Mexicans: Implications for the Study of Genetic Diversity in Admixed Populations - Zúñiga, Joaquín; Yu, Neng; Barquera, Rodrigo; Alosco, Sharon; Ohashi, Marina; Lebedeva, Tatiana; Acuña-Alonzo, Víctor; Yunis, María; Granados-Montiel, Julio; Cruz-Lagunas, Alfredo; Vargas-Alarcón, Gilberto; Rodríguez-Reyna, Tatiana S.; Fernandez-Viña, Marcelo; Granados, Julio; Yunis, Edmond J.
Major histocompatibility complex (MHC) genes are highly polymorphic and informative in disease association, transplantation, and population genetics studies with particular importance in the understanding of human population diversity and evolution. The aim of this study was to describe the HLA diversity in Mexican admixed individuals. We studied the polymorphism of MHC class I (HLA-A, -B, -C), and class II (HLA-DRB1, -DQB1) genes using high-resolution sequence based typing (SBT) method and we structured the blocks and conserved extended haplotypes (CEHs) in 234 non-related admixed Mexican individuals (468 haplotypes) by a maximum likelihood method. We found that HLA blocks and CEHs are...