Limited genetic structure of Gulf Menhaden (Brevoortia patronus), as revealed by microsatellite markers developed for the genus Brevoortia (Clupeidae) - Anderson, Joel D.; Karel, William J.
Long-term sustainable management of wild populations should be based on management actions that account for the genetic structure among populations. Knowledge of genetic structure and of the degree of demographic exchange between discreet [sic] populations allows managers to better define management units. However, adequate gene loci for population assessments are not always available. In this study, variable co-dominant DNA loci in the heavily exploited marine genus Brevoortia were developed with a microsatellite-enriched DNA library for the Gulf Menhaden (Brevoortia patronus). Microsatellite marker discovery was followed by genetic characterization of 4 endemic North American Brevoortia species, by using 14 novel loci...
Population genetics of Cobia (Rachycentron canadum): implications for fishery management along the coast of the southeastern United States - Darden, Tanya L.; Walker, Matthew J.; Brenkert, Karl; Yost, Justin R.; Denson, Michael R.
Cobia (Rachycentron canadum) is a pelagic, migratory species with a transoceanic distribution in tropical and subtropical waters. Recreational fishing pressure on Cobia in the United States has increased substantially during the last decade, especially in areas of its annual inshore aggregations, making this species potentially susceptible to overfishing. Although Cobia along the Atlantic and Gulf coasts of the southeastern United States are currently managed as a single fishery, the genetic composition of Cobias in these areas is unclear. On the basis of a robust microsatellite data set from collections along the U.S. Atlantic coast (2008–09), offshore groups were genetically homogenous....
Triem els amics - Bueno i Torrens, David, 1965-
Les persones tenim tendència a establir amistats amb persones que tenen unes mateixes variants gènetiques.
Resistance of cool season food legumes to Ascochyta blight - Rubiales, Diego; Fondevilla, S.
Legume cultivation is strongly hampered by the occurrence of Ascochyta blights. Strategies of control have been developed but only marginal successes have been achieved. In the present paper we present and re-evaluate some recent developments in control in the light of recent developments in crop breeding and molecular genetics. The current focus in applied breeding is leveraging biotechnological tools to develop more and better markers to speed up the delivery of improved cultivars to the farmer. To date, however, progress in marker development and delivery of useful markers has been slow. The application of knowledge gained from basic genomic research...
High-Throughput Sequencing of RNA Silencing-Associated Small RNAs in Olive (Olea europaea L.) - Donaire, L.; Pedrola, L.; Rosa, R. de la; Llave, C.
Small RNAs (sRNAs) of 20 to 25 nucleotides (nt) in length maintain genome integrity and control gene expression in a multitude of developmental and physiological processes. Despite RNA silencing has been primarily studied in model plants, the advent of high-throughput sequencing technologies has enabled profiling of the sRNA component of more than 40 plant species. Here, we used deep sequencing and molecular methods to report the first inventory of sRNAs in olive (Olea europaea L.). sRNA libraries prepared from juvenile and adult shoots revealed that the 24-nt class dominates the sRNA transcriptome and atypically accumulates to levels never seen in...
Laforin, the most common protein mutated in Lafora disease, regulates autophagy - Aguado, Carmen; Criado-García, Olga; Vernia, Santiago; Boya Tremoleda, Patricia; Sanz, Pascual; Rodríguez de Córdoba, Santiago; Knecht, Erwin; Rubinsztein, David C.
Lafora disease (LD) is an autosomal recessive, progressive myoclonus epilepsy, which is characterized by the accumulation of polyglucosan inclusion bodies, called Lafora bodies, in the cytoplasm of cells in the central nervous system and in many other organs. However, it is unclear at the moment whether Lafora bodies are the cause of the disease, or whether they are secondary consequences of a primary metabolic alteration. Here we describe that the major genetic lesion that causes LD, loss-of-function of the protein laforin, impairs autophagy. This phenomenon is confirmed in cell lines from human patients, mouse embryonic fibroblasts from laforin knockout mice...
In Vitro Reconstitution of Human B-Cell Ontogeny: From CD34+ Multipotent Progenitors to Ig-Secreting Cells - Fluckiger, Anne-Catherine; Sanz, Eva; Garcia-Lloret, Maria; Su, Thomas; Hao, Qian-Lin; Kato, Roberta; Quan, Shirley; Hera, Antonio de la; Crooks, Gay M; Witte, Owen N; Rawlings, David J
We describe a long-term, in vitro culture system initiated with CD34+ or CD34+CD38−umbilical cord blood hematopoietic progenitors that supports normal human B-lineage development, including the production of mature Ig-secreting B cells. In the first stage (human B-progenitor long-term culture [HB-LTC]), CD34+ hematopoietic progenitors are cultured on the murine stromal cell line, S17, leading to the sustained production of large numbers of CD10+, CD19+early B progenitors. Reverse transcriptase-polymerase chain reaction (RT-PCR) and three-parameter flow cytometry for VpreB (surrogate light chain), cytoplasmic μ chain, and surface IgM expression were used to characterize the CD19+ B progenitors present within these cultures. This analysis...
Geoquímica de las aguas termales con CO2 del SE de las Cordilleras Béticas - Cerón García, Juan Carlos; Martín Vallejo, M.; García Rosell, L.
El hidrotermalismo de las zonas alpinas peninsulares ha sido estudiado bajo diferentes
puntos de vista, como son el condicionamiento geológico, la definición y sistematización
de sus facies químicas y las temperaturas de base, entre otros, mostrando siempre su
notable desarrollo y conexión con anomalías geotérmicas de carácter regional. Una de
las cuestiones que parece revestir implicaciones genéticas importantes, así como para la
utilización de estas aguas, es su contenido en dióxido de carbono. Se han estudiado veintisiete
parámetros fisicoquímicos en veintidós puntos de agua sobre un inventario de cincuenta.
La mayoría de los puntos acuíferos estudiados tienen una elevada temperatura,
comprendida entre 20 y 41°C. Las aguas tienen...
Mineralogía, geoquímica y evolución diagenética de los materiales carbonatados del área de Mérida (Badajoz) - Fernández Caliani, Juan Carlos; Galán Huertos, Emilio; Liso, M. J.
En el área de Mérida aflora una serie carbonatada, atribuida al Cámbrico inferior,
compuesta por calizas carbonosas con intercalaciones de niveles y nódulos de chert, en
su tramo inferior, y una secuencia predominantemente dolomítica en el superior. Las
calizas presentan una composición química y mineralógica relativamente homogénea. En
cambio, las dolomías se han diferenciado en tres grupos (dolomías negras, de grano fino;
dolomías blanco-grisáceas, bandeadas; y dolomías oquerosas, de colores pardos y rojizos),
con unos rasgos petrográficos y geoquímicos distintivos. Las relaciones Sr/Fe y
Sr/Mn han permitido estimar el grado de dolomitización de estas rocas y reconocer una
evolución geoquímica compatible con un modelo genético a partir de...
Glucose-starved cells do not engage in prosurvival autophagy - Ramirez-Peinado, Silvia; Leon-Annicchiarico, Clara Lucia; Galindo-Moreno, Javier; Iurlaro, Rafaella; Caro-Maldonado, Alfredo; Prehn, Jochen H.M.; Ryan, Kevin M.; Munoz-Pinedo, Cristina
In response to nutrient shortage or organelle damage, cells undergo macroautophagy. Starvation of glucose, an essential nutrient, is thought to promote autophagy in mammalian cells. We thus aimed to determine the role of autophagy in cell death induced by glucose deprivation. Glucose withdrawal induces cell death that can occur by apoptosis (in Bax, Bak-deficient mouse embryonic fibroblasts or HeLa cells) or by necrosis (in Rh4 rhabdomyosarcoma cells). Inhibition of autophagy by chemical or genetic means by using 3-methyladenine, chloroquine, a dominant negative form of ATG4B or silencing Beclin-1, Atg7, or p62 indicated that macroautophagy does not protect cells undergoing necrosis...
Local adaptation with high gene flow: temperature parameters drive adaptation to altitude in the common frog (Rana temporaria) - Muir, A.P.; Biek, R.; Thomas, R.; Mable, B.K.
Both environmental and genetic influences can result in phenotypic variation. Quantifying the relative contributions of local adaptation and phenotypic plasticity to phenotypes is key to understanding the effect of environmental variation on populations. Identifying the selective pressures that drive divergence is an important, but often lacking, next step. High gene flow between high- and low-altitude common frog (Rana temporaria) breeding sites has previously been demonstrated in Scotland. The aim of this study was to assess whether local adaptation occurs in the face of high gene flow and to identify potential environmental selection pressures that drive adaptation. Phenotypic variation in larval...
The COMPASS subunit Spp1 links histone methylation to initiation of meiotic recombination - Acquaviva,L; Szekvolgyi,L; Dichtl,B; Dichtl,B.S; de La Roche Saint Andre; Nicolas,A; Geli,V
During meiosis, combinatorial associations of genetic traits arise from homologous recombination between parental chromosomes. Histone H3 lysine 4 trimethylation marks meiotic recombination hotspots in yeast and mammals, but how this ubiquitous chromatin modification relates to the initiation of double-strand breaks (DSBs) dependent on Spo11 remains unknown. Here, we show that the tethering of a PHD-containing protein, Spp1 (a component of the COMPASS complex), to recombinationally cold regions is sufficient to induce DSB formation. Furthermore, we found that Spp1 physically interacts with Mer2, a key protein of the differentiated chromosomal axis required for DSB formation. Thus, by interacting with H3K4me3 and...
Genotoxicity of multi-walled carbon nanotubes at occupationally relevant doses - Siegrist, Katelyn J; Reynolds, Steven H; Kashon, Michael L; Lowry, David T; Dong, Chenbo; Hubbs, Ann F; Young, Shih-Houng; Salisbury, Jeffrey L; Porter, Dale W; Benkovic, Stanley A; McCawley, Michael; Keane, Michael J; Mastovich, John T; Bunker, Kristin L; Cena, Lorenzo G; Sparrow, Mark C; Sturgeon, Jacqueline L; Dinu, Cerasela Zoica; Sargent, Linda M
Carbon nanotubes are commercially-important products of nanotechnology; however, their low density and small size makes carbon nanotube respiratory exposures likely during their production or processing. We have previously shown mitotic spindle aberrations in cultured primary and immortalized human airway epithelial cells exposed to single-walled carbon nanotubes (SWCNT). In this study, we examined whether multi-walled carbon nanotubes (MWCNT) cause mitotic spindle damage in cultured cells at doses equivalent to 34 years of exposure at the NIOSH Recommended Exposure Limit (REL). MWCNT induced a dose responsive increase in disrupted centrosomes, abnormal mitotic spindles and aneuploid chromosome number 24 hours after exposure to...
The miR-223/Nuclear Factor I-A Axis Regulates Glial Precursor Proliferation and Tumorigenesis in the CNS - Glasgow, Stacey M.; Laug, Dylan; Brawley, Vita S.; Zhang, Zhiyuan; Corder, Amanda; Yin, Zheng; Wong, Stephen T. C.; Li, Xiao-Nan; Foster, Aaron E.; Ahmed, Nabil; Deneen, Benjamin
Contemporary views of tumorigenesis regard its inception as a convergence of genetic mutation and developmental context. Glioma is the most common and deadly malignancy in the CNS; therefore, understanding how regulators of glial development contribute to its formation remains a key question. Previously we identified nuclear factor I-A (NFIA) as a key regulator of developmental gliogenesis, while miR-223 has been shown to repress NFIA expression in other systems. Using this relationship as a starting point, we found that miR-223 can suppress glial precursor proliferation via repression of NFIA during chick spinal cord development. This relationship is conserved in glioma, as...