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Nomenclatura Unesco > (24) Ciencias de la Vida > (2409) Genética
(2409.01) Embriología (2409.02) Ingeniería genética
(2409.03) Genética de poblaciones (2409.90) Citogenética animal
(2409.91) Genética del desarrollo (2409.92) Genética molecular de plantas
(2409.93) Genética molecular (2409.94) Genética molecular de levaduras
(2409.99) Otras (especificar)

Categorías relacionadas:
(3201.02) Genética clínica (2410.07) Genética humana
(2407.02) Citogenética

Mostrando recursos 1 - 20 de 239,029

1. Distância genética e geográfica entre acessos de picão-preto suscetíveis e reistentes a herbicidas inibidores da acetolactato sintase - Lamego, Fabiane Pinto; Resende, Luciane Vilela; Silva, Paulo Roberto da; Vidal, Ribas Antonio; Nunes, Anderson Luis
O objetivo deste trabalho foi avaliar o grau de similaridade genética entre acessos de picão-preto, suscetíveis e resistentes aos herbicidas inibidores da enzima acetolactato sintase (ALS) e a relação entre similaridade genética e distância geográfica desses acessos. Sementes dos acessos foram coletadas no Estado do Paraná e cultivadas em casa de vegetação, na Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, em outubro de 2004. Depois da confirmação da resistência ou suscetibilidade dos acessos aos inibidores da enzima ALS, realizou-se a extração de DNA. Por meio da técnica de RAPD, foi possível avaliar a similaridade genética entre os...

2. Multi-objective optimization by genetic algorithms in H∞/LPV control of semi-active suspension - Do, Anh Lam; Sename, Olivier; Dugard, Luc; Boussaad, Soualmi
International audience

3. Diversidade genética estimada através de marcadores ISSR de Colletotrichum graminicola no Brasil. - COSTA, R. V. da; SILVA, D. D. da; COTA, L. V.; PARREIRA, D. F.; ZAMBOLIM, L.; GOMES, E. A.; LANA, U. G. de P.; NEVES, W. dos S.; FIGUEIREDO, J. E. F.
A antracnose do milho causada pelo fungo Colletotrichum graminicola é uma das principais doenças da cultura no Brasil e no mundo, atacando praticamente todas as partes da planta. Neste trabalho foi avaliada a variabilidade genética de 95 isolados monospóricos de C. graminicola, provenientes dos estados brasileiros de Goiás, Minas Gerais, Santa Catarina, São Paulo, Paraná e Rio Grande do Sul. O DNA de cada isolado foi amplificado via PCR, utilizando-se 15 primers ISSR (do inglês, inter-simpled sequence repeat) como marcadores moleculares. Os fragmentos de DNA gerados pelo PCR-ISSR foram avaliados por eletroforese em gel de agarose com a visualização da...

4. Procedimiento numérico para la calibración de un modelo de espectro de fuente para la obtención de funciones de atenuación y su aplicación en Colombia - Bernal Granados, Gabriel Andres; Ordaz Schroeder, Mario Gustavo; Salgado Gálvez, Mario Andrés; Cardona Arboleda, Omar Dario; Barbat Barbat, Horia Alejandro
A numerical procedure for the calibration of a source spectrum model to find the optimal combination of seismological parameters and derive unbiased ground motion prediction equations is presented, using available accelerogram recordings as calibration target. The source spectrum model is used to compute the radiated spectra of SH- waves for different seismic moments and hypocentral distances, combining point (far field) and finite (near field) source models. The expected value of the peak ground acceleration is computed from the radiated spectra using random vibrations theory. A genetic algorithm is used to search for the source spectrum parameters that best fit the available...

5. Molecular testing for fragile X: Analysis of 5062 tests from 1105 fragile X families - Performed in 12 clinical laboratories in Spain - Tejada, M. I.; Martínez, Francisco; Fernández Carvajal, Mª Isabel; Ayuso, Carmen; Milà, Montserrat
Fragile X syndrome is the most common inherited form of intellectual disability. Here we report on a study based on a collaborative registry, involving 12 Spanish centres, of molecular diagnostic tests in 1105 fragile X families comprising 5062 individuals, of whom, 1655 carried a full mutation or were mosaic, three cases had deletions, 1840 had a premutation, and 102 had intermediate alleles. Two patients with the full mutation also had Klinefelter syndrome. We have used this registry to assess the risk of expansion from parents to children. From mothers with premutation, the overall rate of allele expansion to full mutation...

6. Functional and molecular characterization of inherited platelet disorders in the Iberian Peninsula: results from a collaborative study - Sánchez-Guiu, Isabel; González-Manchón, Consuelo; Lozano, María L.
Background The diagnostic evaluation of inherited platelet disorders (IPDs) is complicated and time-consuming, resulting in a relevant number of undiagnosed and incorrectly classified patients. In order to evaluate the spectrum of IPDs in individuals with clinical suspicion of these disorders, and to provide a diagnostic tool to centers not having access to specific platelets studies, we established the project “Functional and Molecular Characterization of Patients with Inherited Platelet Disorders” under the scientific sponsorship of the Spanish Society of Thrombosis and Haemostasis. Patients/methods Subjects were patients from a prospective cohort of individuals referred for clinical suspicion of IPDs as well as healthy controls. Functional...

7. Lost P1 allele in sh2 sweet corn: Quantitative effects of p1 and a1 genes on concentrations of maysin, apimaysin, methoxymaysin, and chlorogenic acid in maize silk - Guo, B.Z.; Zhang, Z.J.; Butrón Gómez, Ana María; Widstrom, N.W.; Snook, M.E.; Lynch, R.E.; Plaisted, D.
In the United States, insecticide is used extensively in the production of sweet corn due to consumer demand for zero damage to ears and to a sweet corn genetic base with little or no resistance to ear-feeding insects. Growers in the southern United States depend on scheduled pesticide applications to control ear-feeding insects. In a study of quantitative genetic control over silk maysin, AM-maysin (apimaysin and methoxymaysin), and chlorogenic acid contents in an F2 population derived from GE37 (dent corn, P1A1) and 565 (sh2 sweet corn, p1a1), we demonstrate that the P1 allele from field corn, which was selected against...

8. Percepción vs. inteligencia - Roldán, Luis Ángel
El objetivo del presente trabajo es retomar la teorización que realiza Piaget de la percepción, enfocándonos fundamentalmente en las críticas que hace el autor ginebrino a la Psicología de la Gestalt en su conceptualización de la percepción, con la cual discute la existencia de estructuras de conocimiento a prior, porque consideramos que dichas criticas tienen una profunda vigencia en ciertos debates actuales de la psicología general y de la psicología del desarrollo. Hablar de la percepción desde la óptica de Piaget, requiere primero analizar en qué marco se inscribe dicha concepción de este proceso psicológico básico. No es posible entender...

9. Caracterização do perfil de crises epiléticas e dos efeitos comportamentais induzidos por pilocarpina em peixe-zebra adulto - Pinto, Charles Budaszewski
Epilepsia é um grupo de desordens neurológicas caracterizada por crises epilépticas recorrentes não provocadas, afligindo 50 milhões de pessoas no mundo. Embora grande parte dos casos possa ser controlada através de medicação um número significativo de pacientes torna-se refratários apesar do tratamento com drogas anticonvulsivantes. Apesar do desenvolvimento bemsucedido de várias novas drogas antiepilépticas, a busca por novas terapias com maior eficácia e tolerabilidade ainda permanece um objetivo importante. O modelo de epilepsia induzido por pilocarpina tem sido amplamente utilizado em roedores para o estudo da patogênese da epilepsia do lobo temporal. Embora estes estudos com modelos animais tenham aumentado...

10. A novel forward genetic screen to identify respiratory complex I mutants in Chlamydomonas - Massoz, Simon; Cardol, Pierre; Remacle, Claire; Larosa, Véronique; bailleul, benjamin

11. Correction of the misclassification of species in the Portuguese collection of Cucurbita pepo L. using DNA markers - Rodrigues, R; Veiga, I; Marreiros, A; Rocha, F; Leitao, J
In this study, the genetic variability among 130 accessions of the Portuguese germplasm collection of Cucurbita pepo L. maintained at the Banco Portugues de Germoplasma Vegetal was assessed using AFLP (amplified fragment length polymorphism) and RAPD (random amplified polymorphic DNA) techniques for the identification of a genetically diverse core group of accessions for field phenotypic analysis. The surprisingly completely different molecular patterns exhibited by multiple accessions was later confirmed in the distribution of the putative C. pepo plants into two clusters drastically separated at a very low level of genetic similarity (DICE coefficient = 0.37). Additional analyses with RAPD and...

12. A genetic map of pineapple (Ananas comosus (L.) Merr.) including SCAR, CAPS, SSR and EST-SSR markers - Carlier, JD; Sousa, NH; Santo, TE; d'Eeckenbrugge, GC; Leitao, JM
Despite the paramount importance of pineapple (Ananas comosus L.) in world production and trade of tropical fruits, the genomics of this crop is still lagging behind that of other tropical fruit crops such as banana or papaya. A genetic map of pineapple was constructed using an F2 segregating population obtained from a single selfed F1 plant of a cross A. comosus var. comosus (cv. Rondon, clone BR 50) x A. comosus var. bracteatus (Branco do mato, clone BR 20). Multiple randomly amplified markers (RAPD, ISSR and AFLP) were brought together with SSR and EST-SSR markers identified among sequences uploaded to...

13. Assessment of the genetic relationships among citrus species and varieties by isozyme and RAPD markers - Cabrita, L; Elisiario, P; Leitao, J; Guerreiro, A
A group of 22 Citrus varieties (sweet oranges, lemons, grapefruits, clementines and several other mandarin biotypes) was analysed by isozyme and RAPD markers in order to assess their genetic relationships. Four isozyme systems (GOT, IDH, PGM and PGI) revealed seven loci, four of them polymorphic. Sweet oranges, lemons, grapefruits and clementines were discriminated at the species level but individual varieties within each species remained undistinguishable by the analysed isozyme systems. Nevertheless, a specific isozyme pattern was found for almost each one of the mandarin varieties with the exception of Carvalhais and Fremont, which shared an identical isozyme pattern. Additionally, RAPD...

14. Genetic diversity assessment of the almond (Prunus dulcis (Mill.) DA Webb) traditional germplasm of Algarve, Portugal, using molecular markers - Cabrita, L; Apostolova, E; Neves, A; Marreiros, A; Leitao, J
In this study, 123 almond (Prunus dulcis (Mill.) D. A. Webb) trees identified among traditional orchards in the Algarve region and 53 trees of the local field collection managed by the regional office of the Portuguese Ministry of Agriculture (DRAALG) were assessed using isozyme, inter- single sequence repeat and simple sequence repeat or microsatellite techniques for the evaluation of genetic diversity and genetic relatedness and identification of new accessions for the field collection. The isozyme analysis allowed the distribution of the 176 plants into 13 different classes of enzyme similarity, while the use of DNA markers increased the distribution of...

15. The downy mildew resistance locus Pp523 is located on chromosome C8 of Brassica oleracea L. - Carlier, JD; Alabaca, CA; Coelho, PS; Monteiro, AA; Leitao, JM
We have previously constructed a genetic map of Brassica oleracea L. containing the Pp523 locus that confers downy mildew resistance to adult plants. In this work, 44 SSR markers of reference for the Brassica C genome chromosomes were added to the map, allowing the nine major linkage groups to be assigned to the nine chromosomes of B. oleracea. Locus Pp523 was located on chromosome C8, and a locus determining flower colour was mapped to chromosome C3. In comparison with the first version of the map, the new map is denser and more compact. The available genomic information on B. oleracea...

16. Comparing RAPD and AFLP (TM) analysis in discrimination and estimation of genetic similarities among apple (Malus domestica Borkh.) cultivars - RAPD and AFLP analysis of apples - Goulao, L; Cabrita, L; Oliveira, C; Leitao, JM
Forty-one apple ( Malus x domestica Borkh.) cultivars were screened for RAPD (Random Amplified Polymorphic DNA) and AFLP (Amplified Fragment Length Polymorphism) markers. RAPD analysis was performed with 35 arbitrary 10-mer primers, selected from 60 primers tested (kits A, C and E, Operon Technologies, Inc.). Of a total of 362 bands observed, 208 (57.5%) were polymorphic. Three-hundred-and-eighty-one AFLP fragments were obtained with 8 primer combinations, of which 218 (57.2%) were polymorphic. Cultivars differentiated through mutation were included in this study and showed identical patterns when analysed with both RAPD and AFLP analysis. The estimated genetic relationships were correlated (r =...

17. Suitability of isozyme, RAPD and AFLP markers to assess genetic differences and relatedness among fig (Ficus carica L.) clones - Cabrita, LF; Aksoy, U; Hepaksoy, S; Leitao, JM
Sarilop is the main and standard cultivar for commercial dried fig (Ficus carica L.) production in Turkey. Eleven of the most promising Sarilop clones and one clone of Sarizeybek, all selected from a former agronomic evaluation, were analysed by three molecular marker techniques, isozymes, RAPDs and AFLPs. The resolution power and the accuracy of these three analytical techniques, in distinguishing among fig clones, were determined. The analysis of five isozyme systems permitted the discrimination between the two cultivars, Sarilop and Sarizeybek. Besides the discrimination between the two fig cultivars, the use of 31 10-mer primers in RAPD analysis allowed splitting...

18. An integrated genetic map of pineapple (Ananas comosus (L.) Merr.) - de Sousa, N; Carlier, JD; Santo, T; Leitao, J
The inclusion of multiple new markers, in particular 41 sequence specific markers, resulted in drastically improved version of a previously published F1-based genetic map of pineapple (Ananas comosus). The integration of the new version of this map with a more recently published F2-based map resulted in a map that assembles 741 loci: 739 DNA markers (25 SSR, 12 EST_SSR, 22 SCAR, 8 CAPS, 20 ISSR, 109 RAPD, and 543 AFLP), one isozyme (PGM) locus and the morphological trait 'piping', in 28 integrated linkage groups, spanning 2113 centimorgans (cM) and covering approximately 86% of the genome. Four small F1-based linkage groups...

19. AFLP, ISSR and RAPD markers reveal high levels of genetic diversity among Lupinus spp. - Talhinhas, P; Neves Martins, J; Leitao, J
The Lupinus genus includes a number of important crop species. The use of defined nucleotide sequences for the analysis of genetic diversity among these species has revealed modest levels of diversity. The aim of this study was to access AFLP, ISSR and RAPD markers to evaluate the genetic diversity among L. albus, L. angustifolius, L. cosentinii, L. hispanicus, L. luteus, L. mutabilis, L. pilosus and L. polyphyllus. Unexpectedly, low levels of genetic similarity were found (ranging from 0.205 to 0.432), regardless of the type of molecular marker used. Nevertheless, these techniques consistently showed a greater genetic similarity between L. pilosus...

20. Evaluation of resistance to Phomopsis amygdali in almond - Cabrita, L; Neves, A; Leitao, J
The disease caused by the fungus Phomopsis amygdali (Del.) Tuset & Portilla is, today, one of the most important in almond growing, since the main commercial cultivars are susceptible to this pathogen. In Algarve (Portugal), the disease has been causing severe damage to introduced foreign commercial cultivars while traditional cultivars are not significantly affected. Field observations have led to the identification of 'Barrinho Grado' as a local cultivar that has shown non-susceptible behaviour in a heavily infected orchard. Inoculation tests with Phomopsis amygdali isolates were performed on young trees obtained from open-pollinated seeds of 'Barrinho Grado' (resistant) and 'Ferragnes' (susceptible)....

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