Mostrando recursos 1 - 20 de 3.816


    Carolina Padilha Tavares; Orientadora Profa; Dra Ana; Carvalho Assef; T Tavares; Carolina Padilha; Epidemiologia Molecular I. Título
    Caracterização molecular de Enterobacteriaceae não-Klebsiella pneumoniae produtoras de KPC isoladas em diferentes estados brasileiros Dissertação apresentada ao Instituto Oswaldo Cruz como parte dos requisitos para obtenção do título de Mestre em Biologia Celular e Molecular

  2. Departments of Biología Molecular y Celular1 and Estructura de Macromoléculas,2 Centro Nacional de Biotecnología,

    Capsid Assembly; Jose A. Ruiz-díaz; Jose R. Castón; Florencio Pazos; Jose F. Rodriguez; Centro Empresarial Euronova
    Infectious bursal disease virus (IBDV) capsids are formed by a single protein layer containing three polypeptides, pVP2, VP2, and VP3. Here, we show that the VP3 protein synthesized in insect cells, either after expression of the complete polyprotein or from a VP3 gene construct, is proteolytically degraded, leading to the accumulation of product lacking the 13 C-terminal residues. This finding led to identification of the VP3 oligomerization domain within a 24-amino-acid stretch near the C-terminal end of the polypeptide, partially overlapping the VP1 binding domain. Inactivation of the VP3 oligomerization domain, by either proteolysis or deletion of the polyprotein gene,...

  3. In vitro replication of bacteriophage PRD1 DNA. Characterization of the protein-primed initiation site

    Luis Blanco; Centro De; Biología Molecular; Severo Ochoa; Javier Caldentey; Luis Blanco; Dennis H. Bamford; Margarita Salas
    Bacteriophage PRD1 replicates its DNA by means of a protein-primed replication mechanism. Using single-stranded oligonucleotide templates carrying the sequence corresponding to the 25 first bases of the 3' end of PRD1 DNA, and Mg2+ as the activating metal ion of the phage DNA polymerase, we show that the fourth base from the 3 ' end of the template directs, by base complementarity, the dNMP to be linked to the phage terminal protein (TP) in the initiation reaction. This result suggests that phage PRD1 maintains its 3' end DNA sequences via a sliding-back mechanism. The single-stranded DNA templates could not be...

  4. 2Instituto de Biología Molecular y Celular del Cáncer (IBMCC), CSIC–University of Salamanca,

    Vincent Sauzeau; Inmaculada M. Berenjeno; Carmen Citterio; Xosé R
    The GTPase RhoA participates in a number of cellular processes, including cytoskeletal organization, mitogenesis and tumorigenesis. We have previously shown that the transforming activity of an oncogenic version of RhoA (Q63L mutant) was highly dependent on the transcriptional factor c–Myc. In contrast to these positive effects in the RhoA route, we show here that c–Myc affects negatively the F–actin cytoskeleton induced by RhoAQ63L and its downstream effector, the serine/threonine kinase Rock. This effect entails the activation of a transcriptional program that requires synergistic interactions with RhoA–derived signals and that includes the upregulation of the GTPase Cdc42 and its downstream element...

  5. M.P. de Mello1,2, A.T. Maciel-Guerra2,3,

    Campinas Sp Brasil; G. Guerra Júnior; C. Hackel; C. Hackel
    required for gonadal development are not responsible for XY partial gonadal dysgenesis 1Centro de Biologia Molecular e Engenharia Genética (CBMEG), 2Grupo Interdisciplinar de Estudos da Determinação e Diferenciação do Sexo (GIEDDS), and Departamentos de 3Genética Médica, and 4Pediatria,

  6. Biologia Celular e Molecular

    Cristiane Lima; Orientador Prof; Dra Leila; Mendonça Lima; Prof Dra; Teca Calcagno Galvão
    Análise funcional do regulador transcricional Rv3405c em Mycobacterium bovis BCG


    Faculdade De Biociências; Programa De; Pós-graduação Em; Biologia Celular; E Molecular; Elen Velho Fagherazzi; Drª Nadja Schröder; Porto Alegre; Elen Velho Fagherazzi; Cognitivas Associadas; Ao Acúmulo; De Ferro Cerebral; Em Ratos Wistar
    Uso do Canabidiol como protetor contra disfunções cognitivas associadas ao acúmulo de ferro cerebral em ratos Wistar

  8. Área: Biologia Molecular

    Fátima Leal Seabra; Fátima Leal Seabra; Mestrado Integrado Em Medicina; Prof Doutor; Henrique Manuel; Nunes Almeida; Revista Aging Cell; Fátima Leal-seabra; Liliana Raquel; Casais Matos; Delminda Rosa Gamelas; Henrique Manuel; Nunes Almeida; Henrique Almeida; Md Phd
    Effect of resveratrol in stress-induced premature senescent human fibroblasts

  9. * Corresponding author: Dr. Eduardo Ruiz-Pesini Departamento de Bioquímica, Biología Molecular y Celular

    Aurora Gómez-durán; David Pacheu-grau; Ester López-gallardo; Carmen Díez-sánchez; Julio Montoya; Manuel J. López-pérez; Eduardo Ruiz-pesini
    Unmasking the causes of multifactorial disorders: OXPHOS differences between mitochondrial haplogroups

  10. Laboratorio de Biología Molecular

    Católica Valparaíso; Liliana Cavagnaro; Pablo Masuelli; Ricardo Rodríguez; Facultad De Ciencias Agrarias; Pablo Cavagnaro; Facultad De Ciencias Agrarias; Ricardo Masuelli; José Rodríguez
    Evaluation of diversity among Argentine grapevine (Vitis vinifera L.) varieties using morphological data and AFLP markers


    Presta M; Andres G; Leali D Et Al. Inflammatory; Angiogenesis Eur Cytokine Netw; Marco Presta; Germán Andrés; Centro De; Biología Molecular; Severo Ochoa; See Profile; Roberto Ronca; Marco Presta; Germán Andrés; Daria Leali; Roberto Ronca
    cells and chemokines sustain FGF2-induced


    Inmaculada Tasset; Isaac Túnez; See Profile; Available From Inmaculada Tasset; Inmaculada Tasset; José Peña; Ignacio Jimena; Montserrat Feijóo; María Del Carmen Muñoz; Pedro Montilla; Isaac Túnez; Ciencias Morfológicas; Sección De
    Effect of 17β-estradiol on olfactory bulbectomy-induced oxidative stress and behavioral changes in rats

  13. Samuel Canizales-Quinteros1 and The Metabolic Study Group†

    M. Teresa Villarreal-molina; M. Teresa Flores-dorantes; Olimpia Arellano; Marisela Villalobos-comparan; Maricela Rodríguez-cruz; Angel Miliar; Adriana Huertas-vazquez; Marta Menjivar; Ra Romero-hidalgo; Niels H. Wacher; M. Teresa Tusie-luna; Miguel Cruz; Carlos A. Aguilar-salinas
    *M.T.V.-M. and M.T.F.-D. contributed equally to this work. †A complete list of individuals and institutions participating in the Metabolic Study Group appears in the APPENDIX. Running title: ABCA1 gene variant is associated with early-onset type 2 diabetes Corresponding author: Samuel Canizales-Quinteros Ph.D. Unidad de Biología Molecular y Medicina Genómica

  14. bPrograma de Pós-Graduação em Genética e Biologia Molecular,

    Fa. A; Gp. A; Cm. A; Tro. A
    The goal of conservation biology should be related to the preservation of species and also to the evolutionary and ecological processes that were responsible to form them and that are still acting. We review the conservation status of the species of tuco-tuco (Ctenomys torquatus, C. lami, C. minutus, and C. flamarioni) from southern Brazil, and relate these data to the geological history of a particular area in that region, the Coastal Plain of the States of Rio Grande do Sul and Santa Catarina. The implications of the data on these species from the Southeastern Brazil are also discussed in relation...

  15. Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás,

    Mônica De Oliveira Santos; Nadya Da Silva Castro; Maristela Pereira; Célia Maria; Almeida Soares; Goiânia Goiás Brazil
    This is a report on the analysis of genes involved in translation of the complete genomes of Mycoplasma hyopneumoniae strain J and 7448 and Mycoplasma synoviae. In both genomes 31 ORFs encoding large ribosomal subunit proteins and 19 ORFs encoding small ribosomal subunit proteins were found. Ten ribosomal protein gene clusters encoding 42 ribosomal proteins were found in M. synoviae, while 8 clusters encoding 39 ribosomal proteins were found in both M. hyopneumoniae strains. The L33 gene of the M. hyopneumoniae strain 7448 presented two copies in different locations. The genes encoding initiation factors (IF-1, IF-2 and IF-3), elongation factors...

  16. 1Departamento de Bioquímica, Biología Molecular y Celular; Instituto de Investigación Sanitaria de

    Ester López-gallardo; Sonia Emperador; Abelardo Solano; Laura Llobet; Elena Barraquer; Ivonne Jericó; Eduardo Ruiz-pesini; Julio Montoya D ow nloaded from 2 Mitochondrial DNA mutations at MT-ATP6 gene are relatively common in individuals suffering from striatal necrosis syndromes. These patients usually do not show apparent histochemical and/or biochemical signs of oxidative phosphorylation dysfunction. Because of this, MT-ATP6 is not typically analyzed in many other mitochondrial disorders that have not been previously associated to mutations in this gene. To correct this bias, we have performed a screening of MT-ATP6 gene in a large collection of patients suspected of suffering different mitochondrial DNA disorders. In three cases, biochemical, molecular-genetics and other analyses in patient tissues and cybrids were...


    Faculdade De Biociências; Programa De; Pós-graduação Em; Biologia Celular; E Molecular; Juliane Bentes Picanço; Faculdade De Biociências; Programa De; Pós-graduação Em; Biologia Celular; E Molecular
    Dissertação apresentada como requisito para a obtenção do grau de Mestre pelo Programa de Pós-Graduação em Biologia Celular e Molecular da Faculdade de Biociências da Pontifícia Universidade Católica do Rio Grande

  18. Isolation of Candida famata from a Patient with Acute Zonal Occult

    Centro De; Biología Molecular; Severo Ochoa; See Profile; Maria Eugenia Gonzalez; Luis Carrasco; Marta Ramos; Rebeca Galisteo; Diana Pisa; Manuel Fresno
    The etiology of a number of retinopathies, such as acute zonal occult outer retinopathy (AZOOR), remains undetermined. Candida famata was isolated from conjunctival exudates of a patient diagnosed with AZOOR. This yeast was very abundant, particularly in the more affected eye, while no other pathogens or fungal species were in evidence. Immunological tests revealed the presence of antigen-specific T lymphocytes by using C. famata as a challenge. Moreover, enzyme-linked immunosorbent assay analysis showed the presence of specific antibodies against this yeast in the patient’s blood. Delayed hypersensitivity by use of a skin test was also positive. Finally, antifungal treatments led...

  19. $rec.titulo

    Católica Rio; Grande Sul; Faculdade De Biociências; Associação De; Resistência A Glicocorticóides; E Proli; Feração Espontânea; Em Linfócitos; De Pacientes Infec; Tados Com Htlv-i/ii; Rodrigo Pestana Lopes; Prof Dr; Moisés E. Bauer
    Dissertação apresentada ao Programa de Pós-Graduação em Biologia Celular e Molecular (PPGBCM) como requisito parcial para a obtenção do grau de Mestre


    Presta M; Andres G; Leali D Et Al. Inflammatory; Angiogenesis Eur Cytokine Netw; Marco Presta; Germán Andrés; Centro De; Biología Molecular; Severo Ochoa; See Profile; Roberto Ronca; Marco Presta; Germán Andrés; Daria Leali; Roberto Ronca
    cells and chemokines sustain FGF2-induced

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