Mostrando recursos 41 - 60 de 26,115

  1. SCID/IL2rγnull Mice by Lentiviral Expression of NUP98-HOXA10HD

    Repopulating Hematopoietic; Cells Nod; Allistair Abraham; Yoon-sang Kim; Huifen Zhao; Keith Humphries; A. Persons
    Techniques to expand human hematopoietic stem cells ex-vivo could be beneficial to the fields of clinical hematopoietic stem cell transplantation and gene therapy targeted at hematopoietic stem cells. NUP98-HOXA10HD is a relatively newly discovered fusion gene that in mouse transplant experiments has been shown to increase numbers of hematopoi-etic stem cells. We evaluated whether this fusion gene could be used to expand engrafting human primitive CD34+ cells in an immunodeficient mouse model. Gene transfer was achieved using a lentiviral based vector. The engraftment of mobilized peripheral blood human CD34+ cells grown in culture for one week after gene transfer was...

  2. Address

    Widespread Non-canonical; Sara J. Felts; Virginia P. Van Keulen; Michael J. Hansen; Michael P. Bell; Tanya L. Hoskin; V. Shane Pankratz; Larry R. Pease
    pected changes in the allelic frequency of single nucleotide polymorphisms (SNPs) in cases of C-LOH. However, no evidence of LOH due to mutations, small deletions, or gene conversion at the affected SNPs or surrounding D etic mechanism contributing to V-LOH, we tested wheth tion efficiency using synthetic oligonucleotide templates mat. Methylated templates were systematically over-scor may represent extensive epigenetic DNA modifications ac d do not contain standard (CpG) methylation targets, we p ions occur during Her2/neuT-driven tumorigenesis.

  3. La evolución ética (Die sittliche Evolution) en Wilhelm Dilthey

    Corona Torres, Jesús
    El autor sostiene que la conexión de las vivencias y la configuración de la conexión psíquica se desenvuelven en un proceso que va hacia delante y tiende hacia el perfeccionamiento del conocimiento, del sentir y del querer. En este movimiento el desarrollo de la sociedad juega un papel determinante, pues para nuestro autor la evolución ética se desarrolla exclusivamente mediante el progreso de la sociedad. No obstante, aunque la realización de la bondad es, en gran medida, una decisión y no un sentimiento, lo destacable de este planteamiento es que logra ver la importancia de la vida afectiva en la acción moral, pues ésta depende de los procesos...

  4. Borges como escritor satírico o el difícil equilibrio entre la indignación y la literatura

    Rodríguez Cabello, Pablo
    De todas las formas de lo cómico, la sátira trasluce más las intenciones que la motivan: indignación, rencor, deseos de dominio, en una palabra, el afán de desvalorizar. Lo cómico satírico comporta siempre una crítica de las costumbres, una desvalorización ética, de esta manera, el autor de sátiras se convierte en el portavoz de la moral colectiva. Sin embargo, es necesaria cierta mesura que preserve al autor de caer en la virulencia de lo escatológico. La libertad del humor debe permear toda sátira para que no decaiga estéticamente.

  5. Retórica filosófica y retórica fisiológica

    Álvarez Santos, Remedios
    Se exponen dos tendencias respecto a la concepción de la retórica: la primera se da en torno al planteamiento aristotélico, donde se le considera como una extensión de la filosofía, que se vincula con la dialéctica, la lógica y la ética; la segunda ocurre en torno la la concepción nietzscheana, la cual plantea que la retórica no tiene como fin convencer, sino conmover, además de que se considera que la retórica es la esencia misma del lenguaje, no un ornato.

  6. Bioética en primates no humanos: diagnóstico de los trastornos mentales como argumento para un trato ético completo hacia chimpancés utilizados en espectáculos como mascotas

    Úbeda, Yulán; Llorente, Miquel
    So far, the scarce studies of psychopathology in NHP has been mainly focused on chimpanzees used in biomedical research. Therefore, it is necessary to analyze if it can be equally applied in pet or performer chimpanzees. We believe that these studies will contribute to (1) find the right therapies and (2) to provide ethical fundaments that promote the ending of this procedures. To therefore (3), create a legislative regulation regarding such uses. Taking into account finally, (4) that these studies can provide interesting results from a phylogenetic and comparative perspective on the evolutionary origins of human psychopathology.

  7. La ética del futuro: ¿augura el auge de las neurociencias cambios significativos para la filosofía moral?

    Richart Piqueras, Andrés
    El avance de las neurociencias en la última década ha propiciado una proliferación de disciplinas bajo el rótulo neuro, entre ellas se encuentra la neuroética. Además de las cuestiones éticas relacionadas con la aplicación de las neurociencias y sus métodos, ética de la neurociencia, encontramos que la neurociencia puede aportar algunos datos clave para la investigaciones de las cuestiones clásicas de la filosofía moral, neurociencia de la ética. Algunas de las propuestas en relación a esta última vertiente de la neuroética abogan por la posibilidad de naturalizar la ética en todos sus aspectos. Esta pretensión es problemática y se la...

  8. Mechanistic features of CAG·CTG repeat contractions in cultured cells revealed by a novel genetic assay

    Richard Pelletier; Brian T. Farrell; Juan Jose ́ Miret; Robert S. Lahue
    Trinucleotide repeats (TNRs) undergo high frequency mutagenesis to cause at least 15 neurodegenerative diseases. To understand better the molecular mech-anisms of TNR instability in cultured cells, a new gen-etic assay was created using a shuttle vector. The shuttlevectorcontainsapromoter-TNR-reportergene construct whose expression is dependent on TNR length. The vector harbors the SV40 ori and large T antigen gene, allowing portability between primate cell lines. The shuttle vector is propagated in cultured cells, then recovered and analyzed in yeast using sel-ection for reporter gene expression. We show that (CAGCTG)2533 contracts at frequencies as high as 1 % in 293T and 293 human...

  9. RESEARCH Virulence profile of differe u Background Virulent strains of E. coli mainly belong to phylogen-


    pathogens causing urinary tract infections (UTIs). Phylo-genetic studies have revealed that these bacteria are not of very diverse origin and fall into four main groups (A, B1, B2, and D) [1,2] nowadays. Phylogenetic analysis is usually done by targeting three genetic markers chuA, yjaA genes and DNA fragment TSPE4.C2 [2]. This method has been found satisfactory and endorsed by other workers [3]. etic groups B2 and D, while less virulent and commensal strains belong to phylogenetic groups B1 and A [2-7]. UPEC typically attach to epithelial cells with A-fimbrial adhesins (afa), P-fimbrial adhesins (pap), and S-fimbrial adhesins (sfa) [8-11]. Intimin,...

  10. RESEARCH ARTICLE n

    Harunobu Mori; Kenichi Ohmori; Kazufumi Akiyama
    Cognitive impairments are a core feature of schizophrenia and a major determinant of functional outcome [1]. Cog-nitive domains that are particularly impaired in patients with chronic schizophrenia are verbal memory, working memory, motor speed, verbal fluency, attention, and executive function [2,3]. These cognitive domains, specif-ically working memory and executive function, are heritable traits that have a potential association with gen-etic variants [4,5]. Therefore, these traits are useful as an intermediate phenotype to investigate the functional asso-ciation of susceptibility genes for schizophrenia [6,7]. 22q12 is one of the regions that show high logarithm of the odds ratio scores in schizophrenia [8-10]....

  11. RESEARCH ARTICLE Public Health Genomics e h o


    Full list of author information is available at the end of the articleTraining in the Context of an Enlarging Europe (PHE-TICE) project ” [2], has widely accepted that education and training of public health professionals should include “not only five core components of public health (epidemi-ology, biostatistics, environmental health, health service administration or management, and social and behavioral sciences referring to disease prevention and health promo-tion), but also critical new areas like informatics, genomics, With the advent of personalized healthcare and preven-tion, there is a need to prepare health care providers and public health practitioners to participate in research, evaluation...

  12. Review Cytokines and Growth Hormones

    Activate Stata; Seven Stats
    in mammalian cells.1-3 STAT5A and STAT5B are expressed from two different genes. These are located in tandem on human chro-mosome 17q11.2, and probably arose from gene duplication. The two proteins have approximately 90 kDa and share 94 % amino acid sequence identity, with STAT5A being seven amino acids longer than STAT5B.4,5 STAT5A and STAT5B regulate erythro-poiesis, lymphopoiesis, and the maintenance of the hematopoi-etic stem cell population.2,6-8 The mechanism of STAT activation has been well elabo-rated. Binding of ligands (cytokines or growth factors) to cog-nate receptors activates the associated Janus kinases, JAK1–3 and TYK2 (Fig. 1A). These phosphorylate tyrosine residues of...

  13. $rec.titulo


    Abstract. To identify novel cervical cancer-related genes that are regulated by DNA methylation, integrated analyses of genome-wide DNA methylation and RNA expression profiles were performed using the normal and tumor regions of tissues from four patients; two with cervical cancer and two with pre‑invasive cancer. The present study identified 19 novel cervical cancer-related genes showing differential RNA expression by DNA methylation. A number of the identified genes were novel cervical cancer‑related genes and their differential expression was confirmed in a publicly available database. Among the candidate genes, the epigen-etic regulation and expression of three genes, CAMK2N1, ALDH1A3 and PPP1R3C, was...

  14. The enigmatic monotypic crab plover Dromas ardeola is closely related to pratincoles and coursers (Aves, Charadriiformes, Glareolidae)

    Sergio L. Pereira; Allan J. Baker
    The phylogenetic placement of the monotypic crab plover Dromas ardeola (Aves, Charadriiformes) remains contro-versial. Phylogenetic analysis of anatomical and behavioral traits using phenetic and cladistic methods of tree infer-ence have resulted in conflicting tree topologies, suggesting a close association of Dromas to members of different suborders and lineages within Charadriiformes. Here, we revisited the issue by applying Bayesian and parsimony methods of tree inference to 2,012 anatomical and 5,183 molecular characters to a set of 22 shorebird genera (in-cluding Turnix). Our results suggest that Bayesian analysis of anatomical characters does not resolve the phylogen-etic relationship of shorebirds with strong statistical...

  15. n h

    Cr
    ist of rve up n t eu etic ren ge tes sug vo l RN e T rch nsi rig ka 1. INTR The ea poorly cell stru with fe and ass homolo origins numbe those genes and tr ‘functio eukary three-d nuclear

  16. a


    ryngitis and cervical. Adenitis (PFAPA) was first described as a clinical entity by Marshall et al. in 1987 [1]. PFAPA is an inflammatory disorder mainly of early childhood, with periodic fever accompanied by a predictable set of symp-toms with aphthous stomatitis, pharyngitis and cervical ad-enitis being the most frequent. The most commonly used clinical diagnostic criteria were suggested by Thomas et al. in 1999 [2]. PFAPA has been reported from different ethnic groups [3], and recently we reported a yearly incidence of 2.3 per 10.000 children under the age of five in Norwegian children [4]. The lack of a microbiological...

  17. NKAP is a novel RS-related protein that interacts with RNA and RNA binding proteins

    Bhagyashri D. Burgute; Vivek S. Peche; Anna-lena Steckelberg; Berthold Gaßen; Niels H. Gehring; Angelika A. Noegel
    NKAP is a highly conserved protein with roles in transcriptional repression, T-cell development, mat-uration and acquisition of functional competency and maintenance and survival of adult hematopoi-etic stem cells. Here we report the novel role of NKAP in splicing. With NKAP-specific antibodies we found that NKAP localizes to nuclear speckles. NKAP has an RS motif at the N-terminus followed by a highly basic domain and a DUF 926 domain at the C-terminal region. Deletion analysis showed that the basic domain is important for speckle localization. In pull-down experiments, we identified RNA-binding proteins, RNA helicases and splicing factors as interaction partners of...

  18. Arabidopsis thaliana telomeres exhibit euchromatic features

    Marı́a I. Vaquero-sedas; Francisco M. Gámez-arjona; Miguel A. Vega-palas
    Telomere function is influenced by chromatin struc-ture and organization, which usually involves epigen-etic modifications. We describe here the chromatin structure of Arabidopsis thaliana telomeres. Based on the study of six different epigenetic marks we show that Arabidopsis telomeres exhibit euchro-matic features. In contrast, subtelomeric regions and telomeric sequences present at interstitial chromosomal loci are heterochromatic. Histone methyltransferases and the chromatin remodeling protein DDM1 control subtelomeric heterochromatin formation. Whereas histone methyltransferases are required for histone H3K92Me and non-CpG DNA methylation, DDM1 directs CpG methylation but not H3K92Me or non-CpG methylation. These results argue that both kinds of proteins participate in different...

  19. RESEARCH ARTICLE Granule Associated Serine Proteases of Hematopoietic Cells – An Analysis of Their Appearance and Diversification during Vertebrate Evolution

    Srinivas Akula; Michael Thorpe; Vamsi Boinapally; Lars Hellman
    Serine proteases are among the most abundant granule constituents of several hematopoi-etic cell lineages including mast cells, neutrophils, cytotoxic T cells and NK cells. These pro-teases are stored in their active form in the cytoplasmic granules and in mammals are encoded from four different chromosomal loci: the chymase locus, the met-ase locus, the T cell tryptase and the mast cell tryptase locus. In order to study their appearance during ver-tebrate evolution we have performed a bioinformatic analysis of related genes and gene loci from a large panel of metazoan animals from sea urchins to placental mammals for three of these...

  20. Phylogenetics Advance Access publication January 21, 2014 RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies

    Alexandros Stamatakis
    Motivation: Phylogenies are increasingly used in all fields of medical and biological research. Moreover, because of the next-generation sequencing revolution, datasets used for conducting phylogenetic analyses grow at an unprecedented pace. RAxML (Randomized Axelerated Maximum Likelihood) is a popular program for phylogen-etic analyses of large datasets under maximum likelihood. Since the last RAxML paper in 2006, it has been continuously maintained and extended to accommodate the increasingly growing input datasets and to serve the needs of the user community. Results: I present some of the most notable new features and extensions of RAxML, such as a substantial extension of substitution...

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