http://biblioteca.universia.net/verColeccion.do?id=4005 Mostrando recursos 1 - 20 de 420 es http://biblioteca.universia.net/img/logotipo.jpg http://biblioteca.universia.net/ http://biblioteca.universia.net/ficha.do?id=17584475 The biologically active metabolite of vitamin D3, 1?,25-dihydroxyvitamin D3 [1?,25(OH)2D3], acts through vitamin D receptors, which were found in rheumatoid tissues in the present study. IL-1?-activated rheumatoid synovial fibroblasts and human articular chondrocytes were shown to respond differently to exposure to 1?,25(OH)2D3, which has different effects on the regulatory pathways of specific matrix metalloproteinases and prostaglandin E2. Tetlow, Lynne C; Woolley, David E http://biblioteca.universia.net/ficha.do?id=17584477 Our aim was to identify specifically expressed genes using RNA arbitrarily primed (RAP)-polymerase chain reaction (PCR) for differential display in patients with rheumatoid arthritis (RA). In RA, amplification of a distinct PCR product suitable for sequencing could be observed. Sequence analysis identified the PCR product as highly homologous to a 434 base pair segment of the human centromere kinesin-like protein CENP-E. Differential expression of CENP-E was confirmed by quantitative reverse ... Kullmann , Frank; Judex , Martin; Ballhorn , Wibke; Jüsten , Hans-Peter; Wessinghage , Dieter; Welsh, John; Yen, Tim J; Lang , Bernhard; Hittle, Jim C; McClelland, Michael; Gay, Steffen; Schölmerich, Jürgen; Müller-Ladner, Ulf http://biblioteca.universia.net/ficha.do?id=17584480 Destruction of cartilage and bone are hallmarks of human rheumatoid arthritis (RA), and controlling these erosive processes is the most challenging objective in the treatment of RA. Systemic interleukin-4 treatment of established murine collagen-induced arthritis suppressed disease activity and protected against cartilage and bone destruction. Reduced cartilage pathology was confirmed by both decreased serum cartilage oligomeric matrix protein (COMP) and histological e... Joosten, Leo AB; Lubberts, Erik; Helsen, Monique MA; Saxne, Tore; Coenen-de Roo, Christina JJ; Heinegård, Dick; van den Berg, Wim B http://biblioteca.universia.net/ficha.do?id=17584504 The present study was designed to analyze the level of B-cell clonal diversity in patients with rheumatoid arthritis by using HCDR3 (third complementarity determining region of the rearranged heavy chain variable region gene) length as a marker. A modified immunoglobulin VH gene fingerprinting method using either genomic DNA or complementary (c)DNA derived from B cells of the peripheral blood, synovial fluid, and tissues of several rheumatoid arthritis patients was emp... Itoh, Kenji; Patki, Varsha; Furie, Richard A; Chartash, Elliot K; Jain, Rita I; Lane, Lewis; Asnis, Stanley E; Chiorazzi, Nicholas http://biblioteca.universia.net/ficha.do?id=17584505 In the present study, we searched for mutant PTEN transcripts in aggressive rheumatoid arthritis synovial fibroblasts (RA-SF) and studied the expression of PTEN in RA. By automated sequencing, no evidence for the presence of mutant PTEN transcripts was found. However, in situ hybridization on RA synovium revealed a distinct expression pattern of PTEN, with negligible staining in the lining layer but abundant expression in the sublining. Normal synovial tissue exhibited... Pap, Thomas; Franz, Juliane K; Hummel, Klaus M; Jeisy, Elvira; Gay, Renate; Gay, Steffen http://biblioteca.universia.net/ficha.do?id=17584506 Mast cell (MC) activation in the rheumatoid lesion provides numerous mediators that contribute to inflammatory and degradative processes, especially at sites of cartilage erosion. MC activation in rheumatoid synovial tissue has often been associated with tumour necrosis factor (TNF)-? and interleukin (IL)-1? production by adjacent cell types. By contrast, our in situ and in vitro studies have shown that the production of IL-15 was independent of MC activation, and was ... Woolley, David E; Tetlow, Lynne C http://biblioteca.universia.net/ficha.do?id=17584507 IFN-? was measured in supernatants after in vitro stimulation of peripheral blood mononuclear cells with collagen type II (CII), purified protein derivative or influenza virus. IFN-? production in response to CII was similar in rheumatoid arthritis (RA) patients and healthy control individuals. The IFN-? response to purified protein derivative and influenza virus was lower in RA patients, reflecting a general T-cell hyporesponsiveness in RA. After recalculating the response to CII taking this... Berg, Louise; Rönnelid, Johan; Sanjeevi, Carani B; Lampa, Jon; Klareskog, Lars http://biblioteca.universia.net/ficha.do?id=17584508 Acute-phase serum amyloid A (A-SAA) is a major component of the acute-phase response. A sustained acute-phase response in rheumatoid arthritis (RA) is associated with increased joint damage. A-SAA mRNA expression was confirmed in all samples obtained from patients with RA, but not in normal synovium. A-SAA mRNA expression was also demonstrated in cultured RA synoviocytes. A-SAA protein was identified in the supernatants of primary synoviocyte cultures, and its expressi... O'Hara, Rosemary; Murphy, Evelyn P; Whitehead, Alexander S; FitzGerald, Oliver; Bresnihan, Barry http://biblioteca.universia.net/ficha.do?id=17584509 Serum and synovial tissue expression of the matrix metalloproteinase (MMP)-2 and -9 and their molecular regulators, MMP-14 and TIMP-2 was examined in 28 patients with inflammatory early synovitis and 4 healthy volunteers and correlated with the presence of erosions in the patients. Immunohistological staining of MMP-2, MMP-14 and TIMP-2 localized to corresponding areas in the synovial lining layer and was almost absent in normal synovium. Patients with radiographic erosions had significantly ... Goldbach-Mansky, Raphaela; Lee, Jennifer M; Hoxworth, Joseph M; Smith II, David; Duray, Paul; Schumacher, Ralph H; Yarboro, Cheryl H; Klippel, John; Kleiner, David; El-Gabalawy, Hani S http://biblioteca.universia.net/ficha.do?id=17584510 CD8+ T cells dominate the lymphocyte population in synovial fluid in chronic inflammatory arthritis. It is known that these CD8+ T cells are often clonally or oligoclonally expanded, but their specificity and their relevance to the pathogenesis of joint disease has remained unclear. We found that as many as 15.5% of synovial CD8+ T cells may be specific for a single epitope from an Epstein-Barr virus lytic cycle protein. The virus-specific T cells within the joint showed increased expression ... Tan, Linda C; Mowat, Alastair G; Fazou , Chrysoula; Rostron , Tim; Roskell, Helen; Dunbar , P Rod; Tournay, Claire; Romagné, François; Peyrat, Marie-Alix; Houssaint, Elisabeth; Bonneville, Marc; Rickinson, Alan B; McMichael , Andrew J; Callan, Margaret FC http://biblioteca.universia.net/ficha.do?id=17584511 The kinetics of apoptosis and the apoptosis-regulating gene p53 in adjuvant arthritis (AA) were investigated to assess the value of the AA rat model for testing apoptosis-inducing therapies. Very few terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick end-labeling (TUNEL)-positive cells were detected during the early phases of AA, but on day 23 (chronic arthritis) the percentage of TUNEL-positive cells was significantly increased. Expre... Tak, Paul P; Klapwijk, Maartje S; Broersen, Sophie FM; van de Geest, Deliana A; Overbeek, Marieke; Firestein, Gary S http://biblioteca.universia.net/ficha.do?id=17584512 An inception cohort of 238 patients having peripheral joint synovitis of less than 12 months duration was evaluated clinically and followed prospectively for 1 year to determine the clinical significance of a number of rheumatoid arthritis (RA) associated autoantibodies. Serum samples collected at the time of the initial evaluation were tested for rheumatoid factor (RF) and antibodies to Sa (anti-Sa), RA-33, (pro)filaggrin [antifilaggrin antibody (AFA)], cyclic citrullinated peptide (anti-CCP... Goldbach-Mansky, Raphaela; Lee, Jennifer; McCoy, Angela; Hoxworth, Joseph; Yarboro, Cheryl; Smolen, Josef S; Steiner, Günter; Rosen, Antony; Zhang, Cindy; Ménard, Henri A; Zhou, Zhi Jie; Palosuo, Timo; Van Venrooij, Walther J; Wilder, Ronald L; Klippel, John H; Schumacher, H Ralph; EI-Gabalawy, Hani S http://biblioteca.universia.net/ficha.do?id=17584553 To determine whether IL-4 is therapeutic in treating established experimental arthritis, a recombinant adenovirus carrying the gene that encodes murine IL-4 (Ad-mIL-4) was used for periarticular injection into the ankle joints into mice with established collagen-induced arthritis (CIA). Periarticular injection of Ad-mIL-4 resulted in a reduction in the severity of arthritis and joint swelling compared with saline- and adenoviral control groups. Local expression of IL-4... Kim, Seon Hee; Evans, Christopher H; Kim, Sunyoung; Oligino, Thomas; Ghivizzani, Steven C; Robbins, Paul D http://biblioteca.universia.net/ficha.do?id=17584554 In the present study 55 IgVH genes amplified from three different anatomical regions of a rheumatoid arthritis (RA) patient were analyzed, adding further information on synovial B-cell maturation and recirculation in RA. This analysis demonstrated somatically mutated IgVh genes in all regions studied, with amino acid deletions and mixed IgVh molecules, suggesting the existence of a novel pathway to generate (auto) antibody specificities. Comparison of amino acid sequen... Souto-Carneiro, Maria M; Krenn, Veit; Hermann, Ralph; König, Achim; Müller-Hermelink, Hans-Konrad http://biblioteca.universia.net/ficha.do?id=17584555 The immunodominant T-cell epitope that is involved in collagen-induced arthritis (CIA) is the glycosylated type II collagen (CII) peptide 256-270. In CII transgenic mice, which express the immunodominant CII 256-270 epitope in cartilage, the CII-specific T cells are characterized by a partially tolerant state with low proliferative activity in vitro, but with maintained effector functions, such as IFN-? secretion and ability to provide B cell help. These mice were still susceptible to CIA. Th... Malmström, Vivianne; Bäcklund, Johan; Jansson, Liselotte; Kihlberg, Jan; Holmdahl, Rikard http://biblioteca.universia.net/ficha.do?id=17584556 Autoimmune diseases that are resistant to conventional treatment cause severe morbidity and even mortality. In the present study we demonstrate that complete remissions can be achieved in refractory polychondritis and systemic lupus erythematosus (SLE), even at advanced stage, with the use of autologous stem-cell transplantation (SCT). Remissions persisted after reconstitution of the immune system. In the treatment of advanced systemic sclerosis (SSc), stable disease may be achieved with auto... Rosen, Oliver; Thiel, Andreas; Massenkeil, Gero; Hiepe, Falk; Häupl, Thomas; Radtke, Hartmut; Burmester, Gerd R; Gromnica-Ihle, Erika; Radbruch, Andreas; Arnold, Renate http://biblioteca.universia.net/ficha.do?id=17584557 We propose that the phenomenon of X-chromosome inactivation in females may constitute a risk factor for loss of T-cell tolerance; specifically that skewed X-chromosome inactivation in the thymus may lead to inadequate thymic deletion. Using a DNA methylation assay, we have examined the X-chromosome inactivation patterns in peripheral blood from normal females (n = 30), female patients with a variety of autoimmune diseases (n = 167). No differences between patients and controls were observed. ... Chitnis, Smita; Monteiro, Joanita; Glass, David; Apatoff, Brian; Salmon, Jane; Concannon, Patrick; Gregersen, Peter K http://biblioteca.universia.net/ficha.do?id=17584558 The antigenic specificity of an unusual antinuclear antibody pattern in three patient sera was identified after separating HeLa-cell nuclear extracts by two-dimensional (2D) gel electrophoresis and localizing the antigens by immunoblotting with patient serum. Protein spots were excised from the 2D gel and their contents were analyzed by matrix-assisted laser desorption-ionization (MALDI) or nanoelectrospray ionization time-of-flight (TOF) tandem mass spectrometry (MS) after in-gel digestion w... HH Heegaard, Niels; R Larsen, Martin; Muncrief, Terri; Wiik , Allan; Roepstorff, Peter http://biblioteca.universia.net/ficha.do?id=17584559 IL-10, IL-13, IFN-?, tumor necrosis factor (TNF)-?, LT-?, CD154, and TNF-related activation-induced cytokine (TRANCE) were expressed by 2-20% of rheumatoid arthritis (RA) synovial tissue CD4+ memory T cells, whereas CD4+ cells that produced IL-2, IL-4, or IL-6 were not detected. Expression of none of these molecules by individual CD4+ cells correlated with the exception of TRANCE and IL-10, and TRANCE and TNF-? . A correlation between expression of IL-10 and CCR7, LT-? and CCR6, IFN-? and CCR... Nanki, Toshihiro; E Lipsky, Peter http://biblioteca.universia.net/ficha.do?id=17584560 This study describes the upregulation of neurokinin 1 and bradykinin 2 receptors in dorsal root ganglion (DRG) neurons in the course of antigen-induced arthritis (AIA) in the rat knee. In the acute phase of AIA, which was characterized by pronounced hyperalgesia, there was a substantial bilateral increase in the proportion of lumbar DRG neurons that express neurokinin 1 receptors (activated by substance P) and bradykinin 2 receptors. In the chronic phase the upregulation of bradykinin 2 recep... Segond von Banchet, Gisela; Petrow, Peter K; Bräuer, Rolf; Schaible, Hans-Georg